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24T ELISA Kit Trial Size (Only USD$150/ kit)
|Intra-assay Precision (Precision within an assay): CV%<15%|
|Three samples of known concentration were tested twenty times on one plate to assess.|
|Inter-assay Precision (Precision between assays): CV%<15%|
|Three samples of known concentration were tested in twenty assays to assess.|
|To assess the linearity of the assay, samples were spiked with high concentrations of human ACTH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.|
|The recovery of human ACTH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.|
|Sample Type||Average % Recovery||Range|
|EDTA plasma (n=4)||97||95-100|
|These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.|
The product CSB-E06873h is a sandwich ELISA kit developed to measure concentrations of human ACTH in serum, plasma, or tissue homogenates. This assay uses the sandwich enzyme immunoassay technique in combination with the enzyme-substrate chromogenic reaction to quantify the analyte in the sample. The color develops positively to the amount of ACTH in samples. The color intensity is measured at 450 nm via a microplate reader.
ACTH is an amino acid peptide hormone produced by cells of the anterior pituitary gland and carried by the peripheral circulation to the adrenal cortex, where it stimulates the synthesis and secretion of glucocorticoids and, to a more modest extent, mineralocorticoids and adrenal androgens. As the anterior pituitary mediator of the hypothalamic-pituitary-adrenal axis, ACTH regulates responses to various stressors, including hypoglycemia, psychological stressors such as fear, and physical stressors such as hypovolemia. Upon binding to its receptor MC2R, ACTH acutely activates the intracellular cAMP-PKA pathway, thus increasing the expression of StAR12, which modulates the production of steroids. ACTH deficiency (ACTHD) is caused by disorders of corticotrophs or by disorders of the pituitary stalk or hypothalamus in the case of CRH deficiency and leads to nausea/vomiting, decreased appetite, hypoglycemia, and fatigue.
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