Human adrenocorticotropic hormone (ACTH) ELISA kit

Code CSB-E06873h
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
adrenocorticotropic hormone (ACTH)
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates
Detection Range
12.5 pg/mL-200 pg/mL
12.5 pg/mL
Assay Time
Sample Volume
Detection Wavelength
450 nm
Research Area
Assay Principle
Intra-assay Precision (Precision within an assay): CV%<15%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<15%      
Three samples of known concentration were tested in twenty assays to assess.    
To assess the linearity of the assay, samples were spiked with high concentrations of human ACTH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 87  
Range % 82-93  
1:2 Average % 94  
Range % 89-99  
1:4 Average % 95  
Range % 89-99  
1:8 Average % 92  
Range % 87-96  
The recovery of human ACTH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 92 88-96  
EDTA plasma (n=4) 97 95-100  
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average    
200 0.192 0.187 0.190    
100 0.337 0.328 0.333    
50 0.601 0.613 0.607    
25 1.127 1.140 1.134    
12.5 2.316 2.212 2.264    
and FAQs
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

The product CSB-E06873h is a sandwich ELISA kit developed to measure concentrations of human ACTH in serum, plasma, or tissue homogenates. This assay uses the sandwich enzyme immunoassay technique in combination with the enzyme-substrate chromogenic reaction to quantify the analyte in the sample. The color develops positively to the amount of ACTH in samples. The color intensity is measured at 450 nm via a microplate reader.

ACTH is an amino acid peptide hormone produced by cells of the anterior pituitary gland and carried by the peripheral circulation to the adrenal cortex, where it stimulates the synthesis and secretion of glucocorticoids and, to a more modest extent, mineralocorticoids and adrenal androgens. As the anterior pituitary mediator of the hypothalamic-pituitary-adrenal axis, ACTH regulates responses to various stressors, including hypoglycemia, psychological stressors such as fear, and physical stressors such as hypovolemia. Upon binding to its receptor MC2R, ACTH acutely activates the intracellular cAMP-PKA pathway, thus increasing the expression of StAR12, which modulates the production of steroids. ACTH deficiency (ACTHD) is caused by disorders of corticotrophs or by disorders of the pituitary stalk or hypothalamus in the case of CRH deficiency and leads to nausea/vomiting, decreased appetite, hypoglycemia, and fatigue.

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