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| Code | CSB-EL001827HU |
| Size | 96T,5×96T,10×96T |
| Price | Request a Quote or Start an on-line Chat |
| Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
| To assess the linearity of the assay, samples were spiked with high concentrations of human ANPEP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:20 | Average % | 91 | ||||
| Range % | 84-99 | |||||
| 1:40 | Average % | 91 | ||||
| Range % | 85-96 | |||||
| 1:80 | Average % | 96 | ||||
| Range % | 92-103 | |||||
| 1:160 | Average % | 103 | ||||
| Range % | 96-109 | |||||
| The recovery of human ANPEP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 88-97 | ||||
| EDTA plasma (n=4) | 96 | 90-102 | ||||
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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The Human alanyl (membrane) aminopeptidase (ANPEP) ELISA kit is a reliable and efficient tool for the quantitative detection of Aminopeptidase N (AP-N), also known as hAPN or CD13, in serum, plasma, and tissue homogenates from Homo sapiens (Human) samples.
The ANPEP ELISA kit offers a broad detection range of 31.25 pg/mL to 2000 pg/mL and an impressive sensitivity of 7.81 pg/mL, enabling accurate and precise quantification of AP-N levels in your samples. The assay time is short, ranging from 1 to 5 hours, and requires only a small sample volume of 50-100ul.
The assay principle is a sandwich method, where the AP-N in the sample is captured by a coated antibody and detected by a biotinylated detection antibody, followed by a streptavidin-HRP conjugate, and finally, substrate solution. The signal is detected at a wavelength of 450 nm.
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