Product Details

Uniprot No.

Target Names

Alternative Names

Alanyl (membrane) aminopeptidase antibody; Alanyl aminopeptidase antibody; Aminopeptidase M antibody; Aminopeptidase N antibody; AMPN_HUMAN antibody; ANPEP antibody; AP M antibody; AP N antibody; AP-M antibody; AP-N antibody; APN antibody; CD 13 antibody; CD13 antibody; CD13 antigen antibody; gp150 antibody; hAPN antibody; LAP 1 antibody; LAP1 antibody; Microsomal aminopeptidase antibody; Myeloid plasma membrane glycoprotein CD13 antibody; p150 antibody; PEPN antibody

Species Reactivity

Human

Immunogen

Recombinant Human ANPEP protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Mouse IgG

Clone No.

9C4

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, IF, FC

Recommended Dilution

Application	Recommended Dilution
IF	1:20-1:200
FC	1:20-1:200

Protocols

ELISA Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

ANPEP, also known as CD13 or aminopeptidase N, is a zinc-dependent metalloprotease expressed on the surface of myeloid cells, epithelial cells, and various stem cell populations. This ectoenzyme plays multifaceted roles in peptide processing, cell migration, and signal transduction, making it a valuable marker in immunology research and stem cell biology. As a myeloid plasma membrane glycoprotein, CD13 serves as an important phenotypic marker for characterizing hematopoietic progenitors and investigating myeloid differentiation pathways.

This recombinant monoclonal antibody, clone 9C4, offers the reproducibility and consistency that demanding experimental workflows require. Because the antibody sequence is defined and produced through recombinant expression, researchers benefit from lot-to-lot uniformity that supports longitudinal studies and ensures comparable results across experiments. The mouse IgG2a isotype and affinity-chromatography purification contribute to reliable performance with minimal background interference.

Validation studies demonstrate robust detection of human ANPEP across multiple experimental platforms. Immunofluorescence staining successfully visualized the target in both HEPG2 hepatocellular carcinoma cells and A549 lung adenocarcinoma cells at 1:100 dilution, revealing clear membrane-associated signal when counter-stained with DAPI. Flow cytometry analysis on 293 cells confirmed specific surface detection, with overlay histograms showing distinct positive population shifts compared to isotype control, supporting its utility for cell surface phenotyping applications.

The versatility across immunofluorescence and flow cytometry platforms provides researchers flexibility in experimental design, whether characterizing cell populations, investigating ANPEP expression patterns, or examining its role in cellular processes. This antibody serves as a dependable tool for investigators exploring myeloid biology, stem cell characterization, and related immunological questions.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Broad specificity aminopeptidase which plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. Also involved in the processing of various peptides including peptide hormones, such as angiotensin III and IV, neuropeptides, and chemokines. May also be involved the cleavage of peptides bound to major histocompatibility complex class II molecules of antigen presenting cells. May have a role in angiogenesis and promote cholesterol crystallization. May have a role in amino acid transport by acting as binding partner of amino acid transporter SLC6A19 and regulating its activity.; (Microbial infection) Acts as a receptor for human coronavirus 229E/HCoV-229E. In case of human coronavirus 229E (HCoV-229E) infection, serves as receptor for HCoV-229E spike glycoprotein.; (Microbial infection) Mediates as well Human cytomegalovirus (HCMV) infection.

Gene References into Functions

Crosslinking of CD13 by mAb C or E is required to inhibit adhesion, as monovalent Fab fragments are not sufficient. Thus, C and E antibodies recognize a distinct epitope on CD13, and binding to this epitope interferes with both CD13-mediated cell adhesion and enzymatic activity. PMID: 29789790
Data indicate the expression of aminopeptidase N (CD13) in small cell lung cancer (SCLC) tumor and suggest pre-therapeutic CD13 analysis for testing as investigational predictive biomarker for patient selection. PMID: 29110838
CD13 may be a marker for onychofibroblasts within nail matrix onychodermis PMID: 28708295
This is the first report presenting CD13 and FLI1 as important mediators of resistance to BRAF inhibition with potential as drug targets in BRAF inhibitors refractory melanoma. PMID: 29048432
CD13 enrichment was correlated with early recurrences, and poor prognosis in patients with hepatocellular carcinoma. PMID: 29145632
Patients with acute myeloid leukemia developing leukemia-specific acute hypoxic respiratory failure within 15 days had higher CD13 expression by leukemic cells. PMID: 28323433
Data suggest that CNGRC-GG-D(KLAKLAK)2 peptide actively participates in the downregulation of aminopeptidase N/CD13. PMID: 26655501
CGRP and IL-4 positively regulated APN/CD13 expression and activity in psoriatic fibroblasts. PMID: 28387421
this study shows that CD13 significantly contributes to tissue infiltration by monocytic myeloid-derived suppressor cells and monocytes, thereby contributing to the pathogenesis of hepatic inflammation PMID: 28739875
data reveal a novel role for CD13 in inducing homotypic aggregation in neutrophils, which results in a transmigration deficiency; this mechanism may be relevant to neutrophil micro-aggregation in vivo PMID: 27467268
CD13 expression is associated with hepatoblastoma invasiveness and could be a novel prognostic marker for hepatoblastoma. PMID: 25682862
Data indicate that fluorogenic substrates can be successfully used to identify aminopeptidase N and to measure their activity in cell lysates. PMID: 26449746
Data show that CD13 anntigen and receptor tyrosine kinase-like orphan receptor 2 (ROR2) identify a cardiac lineage precursor pool that is capable of successful engraftment into the porcine heart. PMID: 26771355
The substrate Angiotensin II, the enzymes aminopeptidases-A, B, M as well as IRAP were detected in the jejunal mucosa. PMID: 26311161
14-3-3epsilon might directly bind to CD13, which transmits its signal in chondrocytes to induce a catabolic phenotype similar to that observed in osteoarthritis. PMID: 26208633
Alanyl aminopeptidase expression is confined to mature zymogenic chief cells and its expression is lost en route to metaplasia. PMID: 26514774
Aminopeptidase N activity in tissue and plasma from colorectal cancer patients is an independent prognostic factor of 5-year survival. PMID: 25929234
Expression of APN/CD13 is a potential unfavorable factor to predict the efficacy and prognosis of post-operative chemotherapy in NSCLC patients, especially in lung adenocarcinoma patients. PMID: 25879366
We identified a unique HCC line, Li-7, which not only shows heterogeneity for a CD13(+) CSC hierarchy, but also undergoes a "population change" upon CSC differentiation PMID: 25885470
This study permitted the identification of the novel human LAP1C isoform and partially unraveled the molecular basis of LAP1 regulation. PMID: 25461922
Together these experiments reveal potential enzymatic and signalling roles for APN in osteosarcoma and establish a starting point for an in-depth analysis of the role of APN in regulating invasiveness. PMID: 25340499
Postulate that the interaction of ADAM17 with CD13 and its downregulation following CD13 engagement has important implications in acute myeloid leukemia cells. PMID: 25246708
CD13 could play an important role as a T cell chemoattractant, in a positive feedback loop that contributes to rheumatoid arthritis synovitis. PMID: 25219368
This study showed that CD13 and CD33 expression associated with poor prognosis in patients with MM implicating the need of analysis of these markers in MM diagnosis. PMID: 24991573
Aberrant expression of CD13 in >/= 5% of blasts of patients with SR-aALL is an adverse prognostic factor, delineating a subgroup of patients with SR-aALL that should be considered for more aggressive treatment. PMID: 24411984
Molecular mechanisms regulating CD13-mediated adhesion. PMID: 24627994
CD13 participates in phagocytic processes in dendritic cells and macrophages. PMID: 24063007
Antigen CD13, by influencing the adipogenic potential of human amniotic mesenchymal stem cells, could be an in utero risk factor for obesity. PMID: 23488598
NGR-LDP could bind to CD13-expressing HT-1080 cells. PMID: 23206754
results indicate that APN/CD13 could be an important diagnostic biomarker and therapeutic target for Malignant fibrous histiocytoma . PMID: 23677132
Activities of aminopeptidases N and B and insulin-regulated aminopeptidase could be useful non-invasive biomarkers of Alzheimer's disease from the earliest stages. PMID: 23500679
High CD13 expression is associated with Philadelphia chromosome negative B cell acute lymphoblastic leukemia. PMID: 23643324
results showed that ANPEP downregulation in prostate cancer (PC) was frequently associated with aberrant promoter hypermethylation, suggesting that epigenetic mechanisms are involved in silencing of ANPEP in PC cells PMID: 23322201
CD13 expression plays a role in supporting the survival of cancer stem cells and that there is an epithelial-mesenchymal transition-associated reduction in reactive oxygen species elevation. PMID: 21879266
The hAPN crystal structure provides the first example of a dimeric M1 family member and the observed structural features provide novel insights into the mechanism of peptide processing and signal transduction. PMID: 22932899
Inhibiting CD13 with bestatin may enhance the differentiation-inducing activity of all-trans-retinoic acid in NB4 cells. PMID: 22040956
Coexpression of APN/CD13 correlated significantly with tumor type and neoangiogenesis in breast cancer. PMID: 21611838
Myeloid cell surface peptidase CD13 is highly and specifically expressed on a subset of dendritic cells (DCs) responsible for cross-presentation: transgenic CD8+ murine splenic DCs. PMID: 22544935
The overall effects of APN on the expression of fibronectin, tenascin-C, and MMPs in fibroblasts propose an important role for APN in the regulation of keratinocyte-mediated ECM remodeling and fibroblast contractile activity. PMID: 22065384
Staining of tissue microarrays generated from a large series of melanoma samples and control tissues demonstrated a higher APN expression in primary melanoma lesions when compared with nevi and metastases PMID: 22307972
A peptide-targeted gene vector for highly efficient receptor-mediated intracellular delivery is used to target gene loaded poly(lactic acid)-poly(ethylene glycol) nanoparticles to vascular endothelial cells overexpressing CD13. PMID: 21376765
Murine gamma-herpesvirus-68 glycoprotein (gp)150-specific T-cell receptor epitope transgene activates antiviral CD4+ T cells that are maintained throughout long-term infection and even during quiescent latency. PMID: 22079983
We identified a variant in each of 3 genes (RFC1, SCN1A, ANPEP) potentially implicated in susceptibility to severe neurological complications in West Nile Virus disease. PMID: 21881118
APN expression and enzymatic function is reduced in aggressive meningiomas, and that alterations in the balance between APN and SPARC might favor meningioma invasion PMID: 19236378
analysis of the critical role of flanking residues in NGR-to-isoDGR transition and CD13/integrin receptor switching PMID: 20064928
Stromal aminopeptidase N expression correlates with angiogenesis in non-small-cell lung cancer. PMID: 19908113
data demonstrate that the glycosylation of aminopeptidase N at N291 blocks human coronavirus-229E infection PMID: 11774469
tested the hypothesis that CD13 influences proliferation of monocytoid cells by retarding the velocity of the cell cycle PMID: 11999577
CD13/APN is an important target of Ras signaling in angiogenesis and is a limiting factor in angiogenic progression. PMID: 12406907
inhibiting CD13/aminopeptidase N on the cell-surface of acute promyelocytic leukemia cells increases ATRA-induced differentiation PMID: 12443882

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Subcellular Location

Cell membrane; Single-pass type II membrane protein.

Protein Families

Peptidase M1 family

Tissue Specificity

Expressed in epithelial cells of the kidney, intestine, and respiratory tract; granulocytes, monocytes, fibroblasts, endothelial cells, cerebral pericytes at the blood-brain barrier, synaptic membranes of cells in the CNS. Also expressed in endometrial st

Database Links

HGNC: 500

OMIM: 151530

KEGG: hsa:290

STRING: 9606.ENSP00000300060

UniGene: Hs.1239

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Code	CSB-RA001827MA1HU
Size	US$210
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Image	Immunofluorescence staining of HEPG2 cell with CSB-RA001827MA1HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). Immunofluorescence staining of A549 cell with CSB-RA001827MA1HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). Overlay Peak curve showing 293 cells surface stained with CSB-RA001827MA1HU (red line) at 1:100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1µg/110⁶cells) for 45 min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1µg/110⁶cells) used under the same conditions. Acquisition of >10,026 events was performed.
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ANPEP Recombinant Monoclonal Antibody

Product Details

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Target Background