The generation of this recombinant ANPEP antibody occurs in a series of steps: immunization, splenocytes & PBMC, single B cell sorting, mRNA extraction, RT-PCR & insert vector, expression, ELISA validation. And ELISA, IHC was carried out Every step was performed under strict standards to ensure the researchers can have a recombinant ANPEP antibody with premium quality.
ANPEP is a zinc-dependent metallopeptidase that hydrolyzes the N terminus of biological peptides and extracellular matrix proteins. In mammalians, ANPEP is involved in various cellular processes, such as pain perception, blood pressure modulation, tumor angiogenesis, and metastasis, immune cell chemotaxis, sperm motility, cell-cell adhesion, and coronavirus entry. ANPEP is a target for cancer therapies due to its involvement in tumor cell expansion and motility. Small molecule drugs that bind to the active site of ANPEP impede catalysis and slow tumor growth. ANPEP is also a significant coronavirus cell entrance receptor that binds to a region distant from the active site.