CYC1 Recombinant Monoclonal Antibody

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Datasheet

Code	CSB-RA006327MA1HU
Size	$49.9
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Image	Western Blot Positive WB detected in: A549 whole cell lysate(20µg), PC-3 whole cell lysate(20µg), HeLa whole cell lysate(20µg), K562 whole cell lysate(20µg), HepG2 whole cell lysate(20µg), Jurkat whole cell lysate(20µg), SH-SY5Y whole cell lysate(20µg) All lanes: CYC1 antibody at 1:1000 Secondary Goat polyclonal to human IgG at 1/40000 dilution Predicted band size: 35 kDa Observed band size: 35 kDa Exposure time：1min Overlay Peak curve showing PC-3 cells stained with CSB-RA006327MA1HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was Fluorescein (FITC) AffiniPure Goat Anti-Human IgG, Fcγ fragment specific at 1:200 dilution for 35min at 4℃.Control antibody (green line) was human IgG1 (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Uniprot No.

P08574

Target Names

CYC1

Alternative Names

Complex III subunit 4 antibody; Complex III subunit IV antibody; CY1_HUMAN antibody; CYC1 antibody; Cytochrome b c1 complex subunit 4 antibody; Cytochrome b-c1 complex subunit 4 antibody; Cytochrome bc1 complex subunit 4 antibody; Cytochrome c 1 antibody; Cytochrome c-1 antibody; Cytochrome c1; heme protein; mitochondrial antibody; Ubiquinol cytochrome c reductase complex cytochrome c1 subunit antibody; Ubiquinol-cytochrome-c reductase complex cytochrome c1 subunit antibody; UQCR4 antibody

Species Reactivity

Human

Immunogen

Recombinant Human CYC1 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

hIgG1

Clone No.

27E12D11

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, WB, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

Cytochrome c1 (CYC1) serves as an essential component of the mitochondrial respiratory chain, functioning within Complex III (ubiquinol-cytochrome c reductase) to facilitate electron transfer during oxidative phosphorylation. This protein plays a fundamental role in cellular energy metabolism, making it a valuable marker for studies investigating mitochondrial function, bioenergetics, and metabolic disorders.

This recombinant monoclonal antibody targeting human CYC1 offers researchers the reliability that comes with sequence-defined production. Unlike traditional hybridoma-derived antibodies, recombinant technology ensures consistent performance across lots, eliminating the variability that can compromise longitudinal studies or require repeated optimization. The human IgG1 isotype format and affinity-chromatography purification contribute to a reagent designed for reproducible results.

Validation studies demonstrate robust performance across multiple experimental platforms. Western blot analysis confirms specific detection of CYC1 at the predicted molecular weight of 35 kDa across a diverse panel of human cell lines, including A549, PC-3, HeLa, K562, HepG2, Jurkat, and SH-SY5Y cells at recommended dilutions of 1:500–1:2000. This broad cell line compatibility reflects the ubiquitous expression of mitochondrial respiratory components and provides flexibility for researchers working with various cellular models. Flow cytometry validation in PC-3 cells further demonstrates the antibody's utility for single-cell analysis of intracellular CYC1 expression, with clear separation from isotype control at dilutions of 1:50–1:200.

This antibody supports research into mitochondrial dysfunction, cancer metabolism, neurodegenerative diseases, and cellular bioenergetics where understanding Complex III activity and expression is essential for advancing mechanistic insights.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Component of the ubiquinol-cytochrome c oxidoreductase, a multisubunit transmembrane complex that is part of the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. The cytochrome b-c1 complex catalyzes electron transfer from ubiquinol to cytochrome c, linking this redox reaction to translocation of protons across the mitochondrial inner membrane, with protons being carried across the membrane as hydrogens on the quinol. In the process called Q cycle, 2 protons are consumed from the matrix, 4 protons are released into the intermembrane space and 2 electrons are passed to cytochrome c. Cytochrome c1 is a catalytic core subunit containing a c-type heme. It transfers electrons from the [2Fe-2S] iron-sulfur cluster of the Rieske protein to cytochrome c.

Gene References into Functions

these results indicate that CYC1 plays important roles in cell proliferation and comedo necrosis through the elevated oxidative phosphorylation activity in human ductal carcinoma in situ PMID: 28394473
results indicate that miR-661 plays a tumor suppressor role in OS mediated by the downregulation of CYC1, suggesting a potential mechanism underlying cell death resistance in Osteosarcoma. PMID: 28391262
CYC1 promoted tumor metastasis via suppressing activation of AMPK and contributed to tumor growth via facilitating production of ATP. PMID: 27239088
FASL, granzyme B, and cytochrome c blood expression reflects breast cancer progression and response to therapy. (Review) PMID: 27117663
our results provide evidence that CYC1 plays an important role in OS tumorigenesis, and modulation of CYC1 may be an effective strategy to potentiate OS to apoptotic induction by TRAIL. PMID: 25562155
Mutations in CYC1 cause insulin-responsive hyperglycemia. PMID: 23910460
HeLa cells expressing non-cleavable cytochrome c1 maintain mitochondrial morphology and functions. PMID: 21577235
Decreased electron Transport Complex IV activity is associated with ulcerative colitis. PMID: 20440543
Addition of H202 to tendon fibroblasts in vitro resulted in oxidative stress-induced apoptosis and the release of CYC1. PMID: 12788227
the Ca(2+) spike, mitochondrial Bcl-2 presensitization, and subsequent Delta psi(m) transition, Bax translocation, and ROS generation are important upstream signals for cytochrome c release upon stimulation by a Bcl-2 inhibitor. PMID: 14966123
Sensitivity of Cyt c detection in -linked immunosorbent assay is reduced in the presence of serum leucine-rich alpha-2-glycoprotein-1. PMID: 16699948

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Involvement in disease

Mitochondrial complex III deficiency, nuclear 6 (MC3DN6)

Subcellular Location

Mitochondrion inner membrane; Single-pass membrane protein.

Protein Families

Cytochrome c family

Database Links

HGNC: 2579

OMIM: 123980

KEGG: hsa:1537

STRING: 9606.ENSP00000317159

UniGene: Hs.289271

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

Cardiac muscle contraction Oxidative Phosphorylation

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

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