Human fatty acid binding protein 4,FABP-4 Elisa Kit

Instructions
Code CSB-E12995h
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name fatty acid binding protein 4, adipocyte
Alternative Names 3T3-L1 lipid-binding protein ELISA Kit; 422/aP2 ELISA Kit; A-FABP ELISA Kit; adipocyte ELISA Kit; Adipocyte lipid binding protein ELISA Kit; Adipocyte lipid-binding protein ELISA Kit; Adipocyte protein AP2 ELISA Kit; Adipocyte-type fatty acid-binding protein ELISA Kit; AFABP ELISA Kit; ALBP ELISA Kit; ALBP/Ap2 ELISA Kit; aP2 ELISA Kit; Epididymis secretory protein Li 104 ELISA Kit; FABP ELISA Kit; FABP4 ELISA Kit; FABP4_HUMAN ELISA Kit; Fatty acid binding protein 4 adipocyte ELISA Kit; Fatty acid binding protein 4 ELISA Kit; Fatty acid binding protein adipocyte ELISA Kit; Fatty acid-binding protein 4 ELISA Kit; Fatty acid-binding protein ELISA Kit; HEL S 104 ELISA Kit; Lbpl ELISA Kit; Myelin P2 protein homolog ELISA Kit; P15 ELISA Kit; P2 adipocyte protein ELISA Kit; Protein 422 ELISA Kit
Abbreviation FABP4
Uniprot No. P15090
Species Homo sapiens (Human)
Sample Types serum, plasma
Detection Range 62.5 pg/mL-4000 pg/mL
Sensitivity 15.6 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human FABP-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:20Average %91
Range %86-98
1:40Average %97
Range %92-104
1:80Average %85
Range %81-88
1:160Average %89
Range %83-96
Recovery
The recovery of human FABP-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9287-97
EDTA plasma (n=4)9490-98
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2Average
40000.082 0.086 0.084
20000.145 0.134 0.140
10000.223 0.221 0.222
5000.342 0.324 0.333
2500.582 0.624 0.603
1250.824 0.859 0.842
62.51.208 1.256 1.232
02.051 2.216 2.134
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Data

Function Lipid transport protein in adipocytes. Binds both long chain fatty acids and retinoic acid. Delivers long-chain fatty acids and retinoic acid to their cognate receptors in the nucleus (By similarity).
Gene References into Functions
  1. FABP4 shows potential as a novel biomarker for diabetic retinopathy prediction in Chinese patients with type 2 diabetes (T2DM), and strict glycemic control and more frequent retinal examination should be highlighted for T2DM patients with the highest quartile range of FABP4. PMID: 29305311
  2. association between serum FABP4 concentrations and sarcopenia in chronic hemodialysis patients PMID: 29935964
  3. intermittent high glucose potentiates A-FABP activation and inflammatory responses via TLR4/p-JNK signaling in THP-1 cells PMID: 29850615
  4. High serum A-FABP concentration is associated with peripheral arterial disease in women, but not men, with type 2 diabetes mellitus. PMID: 28303680
  5. FABP4 from adipocytes mediate adipocyte-cholangiocarcinoma interactions that are crucial for the invasion, migration and epithelial-mesenchymal transition of cholangiocarcinoma cells PMID: 29237483
  6. Compared with the low arterial stiffness group, the high arterial stiffness group had higher values for age, systolic blood pressure, pulse pressure, duration of kidney transplantation and serum A-FABP PMID: 28660445
  7. Blood FABP4 is a biomarker for metabolic syndrome and cardiovascular disease independently of HIV status and antiretroviral therapy. PMID: 29304747
  8. Increased FABP4 blood level is a biomarker of lead exposure in women. PMID: 29264639
  9. ubiquitylation destabilizes the fold of two proteins, FKBP12 and FABP4 PMID: 27991582
  10. Compared to control group, breast cancer patients show higher FABP4 and FABP5 blood levels. Our data suggest that, particularly, circulating FABP4 levels could be considered a new independent breast cancer biomarker. Our work translates basic science data to clinic linking the relationship between adipose tissue and lipid metabolism to breast cancer. PMID: 28701570
  11. findings reveal a pro-angiogenic role of FABP4 in first trimester placental trophoblast cells and its regulation may have impact in placental physiology. PMID: 28620819
  12. A-FABP concentration was an independent predictor of cardiovascular events in patients with stable angina undergoing percutaneous coronary intervention. PMID: 29017449
  13. Ectopic expression and secretion of FABP4 in vascular endothelial cells contribute to neointima formation after vascular injury. PMID: 28903937
  14. Elevated A-FABP concentration could be a predictor for MetS and arterial stiffness in hypertensive patients PMID: 28860092
  15. The present study demonstrated that the androgen was correlated with the serum A-FABP levels negatively in men, but positively in women. PMID: 27270834
  16. Increased second trimester plasma FABP4 independently predicted GH/PE in GDM patients PMID: 29394285
  17. our research demonstrated that high FABP3 or FABP4 expression had strong prognostic value for overall survival in non-small cell lung cancer PMID: 27323829
  18. The distribution of genotype frequencies in the assessed women (PON1 Q192R polymorphism) was QQ = 20%, QR = 48% and RR = 32%. Significantly higher serum FABP4 levels were found in women with genotype QR/RR (20.6 +/- 2.20 ng/mL), when compared with the levels found in the QQ group (12.8 +/- 1.70 ng/mL) (p = .004). PMID: 27712128
  19. serum A-FABP levels were increased significantly in normoglycemic individuals with a first-degree family history of diabetes; the use of serum A-FABP as a biomarker in the first-degree relatives of patients with diabetes may result in overestimation of the risk of obesity-induced metabolic disease and cardiovascular disease PMID: 27534843
  20. Findings in the dorsolateral prefrontal cortex in schizophrenia provide evidence of altered proteins involved in synaptic function (FABP4), cytoarchitecture organization (NEFH), and circadian molecular clock signaling (CSNK1E), which may be contributing to the cognitive and/or negative symptoms in this disorder. FABP4, CSNK1E and NEFH could become potentially useful biomarkers for schizophrenia. PMID: 27236410
  21. Circulating levels of AFABP and EFABP are not decreased in Lipodystrophy despite adipose tissue loss in contrast to other adipokines including leptin and adiponectin. PMID: 28088613
  22. Taken together, PPAR gamma and FABP4 gene expression levels in PBMCs [peripheral blood mononuclear cells] may be indicators of metabolic factors and body composition components. PMID: 27563749
  23. eFABP4 induces ER stress and potentiates the effect of linoleic acid in HepG2 cells, suggesting that FABP4 could be a link between obesity-associated metabolic abnormalities and hepatic insulin resistance mechanisms PMID: 27155388
  24. Increased second-trimester FABP4 independently predicted pre-eclampsia in women with type 1 diabete and significantly improved reclassification and discrimination. PMID: 27630211
  25. High levels of FABP4 are significantly related to stroke risk and severity, independent from other traditional and emerging risk factors, suggesting that they may play a role in stroke pathogenesis. PMID: 28781090
  26. We found that serum FABP4 concentration were associated with insulin resistance and secretion in type 2 diabetes mellitus. This suggests that FABP4 may play an important role in glucose homeostasis. PMID: 28654680
  27. High FABP4 expression is associated with ovarian cancer. PMID: 27568980
  28. AFABP levels were higher in neonates compared with adults. Preterm infants had higher AFABP levels compared with full-term infants. Among full-term infants, AFABP levels in SGA infants were lower, compared with appropriate for gestational age and large for gestational age infants. PMID: 28324040
  29. Exogenous FABP4 plays a key role in tumor proliferation and activates the expression of fatty acid transport proteins in MCF-7 breast cancer cells. PMID: 27061264
  30. High FABP4 expression is associated with Acute Monocytic Leukemia. PMID: 28108519
  31. FABP4 over-expression in cardiomyocytes can aggravate the development of cardiac hypertrophy through the activation of ERK signal pathway. PMID: 27294862
  32. Results show that serum FABP4 levels were significantly increased in patients with psoriasis. PMID: 27864793
  33. secreted FABP4 and FABP5 from adipocytes as adipokines differentially affect transcriptional and metabolic regulation in ADSC near adipocytes PMID: 27936164
  34. FABP4 locally produced by epicardial/perivascular fat and macrophages in vascular plaques contributes to the development of coronary atherosclerosis. PMID: 27013610
  35. Elevated levels of FABP4 are associated with higher cardiovascular mortality among men with type 2 diabetes. PMID: 27609367
  36. These genes should be at the downstream of signal pathways and be regulated by the 23 genes identified before. Our findings may provide a unique new model for studying the molecular control of cattle cross-talk between adipose and skeletal muscle. PMID: 27856250
  37. higher serum A-FABP level is positively associated with MetS in type 2 DM patients PMID: 28042581
  38. FABP4 level was independently associated with gender, adiposity, renal dysfunction, and levels of cholesterol. PMID: 27241838
  39. FABP4 DNA hypomethylation induced by Hcy may be involved in the overexpression of FABP4, thereby inducing cholesterol accumulation in macrophages. PMID: 27221153
  40. Study found that the fasting insulin and age-adjusted AFABP concentrations were significantly higher in the gestational diabetes mellitus group of women compared with the normal glucose tolerance participants in the mid- and late stages of pregnancy. PMID: 27181269
  41. mTOR/FABP4 signaling pathway directly regulates the proliferation of endothelial cells in hemangioma. Rapamycin and inhibitors of FABP4 have therapeutic potential for treating infantile hemangiomas. PMID: 27914823
  42. Results suggested that the expression of a lipid metabolism-related gene and an important SNP in the 3'-UTR of FABP4 are associated with TNBC prognosis. PMID: 26959740
  43. In multiple linear regression stepwise analysis, BMI, HbA1C, and HOMA-IR were significantly independent determinants for A-FABP. BMI, HbA1C, and HOMA-IR are independently associated with A-FABP in obese subjects with newly diagnosed type 2 diabetes. A-FABP may be related to insulin resistance and inflammation in type 2 diabetes and concomitant obesity. PMID: 27819006
  44. The increase in placental FABP4 expression in preeclampsia may affect the function of trophoblast, and this increase may have a role in the pathogenesis of preeclampsia. PMID: 26992681
  45. our findings set up a novel mechanism for FABP4, adipsin and adiponectin through gut microbiota mediating expression in gut Paneth cells. PMID: 26687459
  46. results underline a gender difference in FABP4 secretion in stable COPD patients. PMID: 26823558
  47. Increased FABP4 level showed increased risk for reduced lung function in subjects with normal ventilatory function. PMID: 26887419
  48. High FABP expression is associated with Sepsis. PMID: 27089280
  49. Serum irisin might exert antagonistic effects on FABP4 in the middle-aged Chinese population. PMID: 26752184
  50. Urinary FABP4 level is independently correlated with level of albuminuria and possibly predicts yearly decline of estimated glomerular filtration rate. Urinary FABP4 would be a novel biomarker of glomerular damage. PMID: 25506691

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Subcellular Location Cytoplasm, Nucleus
Protein Families Calycin superfamily, Fatty-acid binding protein (FABP) family
Database Links

HGNC: 3559

OMIM: 600434

KEGG: hsa:2167

STRING: 9606.ENSP00000256104

UniGene: Hs.391561

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