Human lactate dehydrogenase A (LDHA) ELISA kit

Instructions
Code CSB-E17850h
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name lactate dehydrogenase A
Alternative Names Cell proliferation-inducing gene 19 protein ELISA Kit; GSD11 ELISA Kit; L lactate dehydrogenase B chain ELISA Kit; L-lactate dehydrogenase A chain ELISA Kit; Lactate dehydrogenase A ELISA Kit; Lactate dehydrogenase B ELISA Kit; Lactate dehydrogenase c variant 1 ELISA Kit; Lactate dehydrogenase c variant 3 ELISA Kit; Lactate dehydrogenase c variant 4 ELISA Kit; Lactate dehydrogenase C4 ELISA Kit; Lactate dehydrogenase H chain ELISA Kit; Lactate dehydrogenase M ELISA Kit; LDH A ELISA Kit; LDH B ELISA Kit; LDH H ELISA Kit; LDH heart subunit ELISA Kit; LDH M ELISA Kit; LDH muscle subunit ELISA Kit; LDH-A ELISA Kit; LDH-M ELISA Kit; LDH1 ELISA Kit; ldha ELISA Kit; LDHA_HUMAN ELISA Kit; LDHBD ELISA Kit; LDHM ELISA Kit; MS1111 ELISA Kit; PIG19 ELISA Kit; Proliferation inducing gene 19 ELISA Kit; Proliferation-inducing gene 19 ELISA Kit; Renal carcinoma antigen NY REN 46 ELISA Kit; Renal carcinoma antigen NY-REN-59 ELISA Kit; TRG 5 ELISA Kit; TRG5 ELISA Kit
Abbreviation LDHA
Uniprot No. P00338
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 31.25 mU/mL-2000 mU/mL
Sensitivity 7.81 mU/Ml
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%        
Three samples of known concentration were tested twenty times on one plate to assess.    
Inter-assay Precision (Precision between assays): CV%<10%        
Three samples of known concentration were tested in twenty assays to assess.      
               
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human LDHA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
  Sample Serum(n=4)    
1:1 Average % 88    
Range % 81-92    
1:2 Average % 99    
Range % 94-102    
1:4 Average % 101    
Range % 97-105    
1:8 Average % 94    
Range % 88-98    
Recovery
The recovery of human LDHA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
 
Sample Type Average % Recovery Range    
Serum (n=5) 93 89-96    
EDTA plasma (n=4) 96 94-98    
               
               
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
 
mU/ml OD1 OD2 Average Corrected    
2000 2.101 1.952 2.027 1.914    
1000 1.676 1.734 1.705 1.592    
500 1.252 1.238 1.245 1.132    
250 0.768 0.758 0.763 0.650    
125 0.566 0.568 0.567 0.454    
62.5 0.328 0.321 0.325 0.212    
31.25 0.179 0.185 0.182 0.069    
0 0.114 0.112 0.113      
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Target Data

Gene References into Functions
  1. The study reveals that miR-33b plays a suppressive role in the regulation of osteosarcoma cell proliferation through direct targeting LDHA. PMID: 30213286
  2. hCINAP determines self-renewal of colorectal cancer stem cells by facilitating LDHA phosphorylation. PMID: 28516914
  3. the results of the present study demonstrated that miR199a3p can inhibit LDHA expression by downregulating Sp1, and provided mechanistic evidence supporting the existence of a novel miR199a3p/Sp1/LDHA axis and its critical contribution to aerobic glycolysis in testicular cancer cells. PMID: 30015851
  4. High LDHA expression is associated with Non-Small Cell Lung Cancer. PMID: 29321091
  5. This is the first study from this highly prevalent region of Head neck cancer showing that serum LDH could be regarded as a biomarker for malignant and premalignant conditions of the head and neck. PMID: 30197328
  6. High LDH expression is associated with small cell lung cancer. PMID: 29970686
  7. The PFK2 expression along with LDH-4 were observed to be increased ~2-fold (P < 0.001) in 0.5 mM ammonia treated brain slices. PMID: 23703029
  8. miR-199a-3p is a major metabolic regulator in tumor suppression and miR-199a-3p may downregulate metabolic genes (LDHA, PGK1, MCT1, TIGAR) through the transcription factor Sp1. PMID: 27432288
  9. The expression of LDH-5 and hypoxia-inducible factor (HIF) 1alpha in Non-Hodgkin's lymphoma, was examined. PMID: 24086384
  10. Results show that LDHA is highly expressed in triple negative breast cancer (TNBC) and correlated with a poor outcome. Its 3'UTR is targeted by miR-34a. Also, LDHA along with PDL1 seem to act as ceRNAs to promote the expression and function of each other through regulation of miR-34a in TNBC. PMID: 28915924
  11. LDHA siRNA can inhibit cell proliferation, induce apoptosis, reduce invasion and migration in renal cell carcinoma cells. PMID: 27027898
  12. JMJD2A regulated aerobic glycolysis by regulating LDHA expression. Therefore, the novel JMJD2A-LDHA signaling pathway could contribute to the Warburg effects in NPC progression. PMID: 28693517
  13. The present study proved that HIF1/2alpha could activate LDHA expression in human pancreatic cancer cells, and high expression of LDHA promoted the growth and migration of pancreatic cancer cells. PMID: 28193910
  14. Pretreatment LDH levels had noticeable prognostic value in advanced pancreatic ductal adenocarcinoma (PDAC) patients who received subsequent chemotherapy. Tackling elevated LDH levels before the initiation of chemotherapy might be a promising measure for improving overall survival of patients after treatment for their advanced PDAC. PMID: 28056913
  15. Authors demonstrated that knock down of LDHA with siRNA or inhibition of LDHA activity with a LDHA specific inhibitor (FX-11), could sensitize PC-3RR cells to radiotherapy with reduced epithelial-mesenchymal transition, hypoxia, DNA repair ability and autophagy, as well as increased DNA double strand breaks and apoptosis. PMID: 27708237
  16. Taken together, these results indicate that miR-142-3p could act as a tumor suppressor in HCC by targeting LDHA, suggesting new therapeutic targets for HCC treatment. PMID: 29360449
  17. Findings suggest that miR-34b-3 and miR-449a suppress the development of nasopharyngeal carcinoma (NPC) through regulation of glycolysis via targeting lactate dehydrogenase A.(LDHA) and may be potential therapeutic targets for the treatment of NPC. PMID: 27458165
  18. Results provide evidence that miR-200b acts as a tumor suppressor in glioma through the inhibition of LDHA both in vitro and in vivo. PMID: 27374173
  19. our data demonstrate that overexpression of 14-3-3zeta in early stage pre-cancerous breast epithelial cells may trigger an elevated glycolysis and transcriptionally up-regulating LDHA, thereby contributes to human breast cancer initiation. PMID: 27150057
  20. serum lactate dehydrogenase has a role in predicting the outcome of resected gastric cancer and construct prognostic nomograms for risk prediction PMID: 27223065
  21. LDHA phosphorylation and activation provide pro-invasive, anti-anoikis and pro-metastatic advantages to cancer cells, suggesting that Y10 phosphorylation of LDHA may represent a promising therapeutic target and a prognostic marker for metastatic human cancers. PMID: 28218905
  22. miR-30a-5p may function as a tumor suppressor in breast cancer through the inhibition of LDHA expression and glycolysis. PMID: 28461244
  23. Overexpression of LDHA can be a marker of poor prognosis in cholangiocarcinoma patients PMID: 27615379
  24. LDHA-associated lactic acid accumulation in melanomas inhibits tumor surveillance by T and natural killer cells. PMID: 27641098
  25. Data indicate that lactate dehydrogenase A (LDHA) is involved in the transcription of histone 2B gene. PMID: 28257841
  26. LDHA downregulation is involved in the apoptotic effect of diallyl trisulfide in triple-negative breast cancer. PMID: 27566995
  27. this study shows that low levels of LDH correlate to a better anti-CTLA-4 immunotherapy response and survival in advanced melanoma patients PMID: 27728898
  28. alternative isoforms of LDH in cancer cells produce lactic acid, when LDHA is silenced or inhibited PMID: 28314283
  29. Overexpression of PKM2 and LDH5 associates with key clinicopathological features and unfavourable prognosis in tongue squamous cell carcinoma. PMID: 24762230
  30. These results suggest LDH-A and/or lactate as common elements at the cross-road between cancer cell metabolism, tumor progression and inflammation PMID: 27622920
  31. High serum LDH is associated with OS and PFS in patients with urinary system cancer, and it is an effective biomarker of prognosis in patients with urologic cancer. PMID: 27710971
  32. High serum LDH level is associated with cancer. PMID: 27511116
  33. The number of in advanced-stage diffuse large B cell lymphoma patients with elevated serum lactate dehydrogenase who achieved complete response (CR) after R-CHOP in the non autologous hematopoietic stem cell transplantation (ASCT) group was higher than that in the ASCT group. PMID: 27324387
  34. this study shows that elevated serum LDH levels are associated with unfavorable prognosis in nasopharyngeal carcinoma patients treated with intensity-modulated radiotherapy PMID: 26928265
  35. LDHA is a direct target of miR-34a in regulating glucose metabolism and tumor growth in breast cancer. PMID: 26902416
  36. IDO, through GCN2 kinase activation, downregulates the levels of TCRcomplex tchain and cMyc, resulting in the suppression of Tcell proliferation and a reduction in the levels of LDHA and GLS2 PMID: 26647830
  37. Findings provide evidence of the cellular functions of lactate dehydrogenase A in the progression of glioblastoma and suggest that lactate dehydrogenase A might act as a potential therapeutic target for glioblastoma treatment. PMID: 26694942
  38. Reanalysis of genome-wide association study and in vitro validation show that lactate dehydrogenase interacts with branched-chain amino acid metabolism. PMID: 26014429
  39. LDH and complex I play distinct roles in regulating glycolysis and cell proliferation. Inhibition of these two augments synthetic lethality in melanoma. PMID: 26484566
  40. Multivariable analysis showed that combined expression of pyruvate kinase type M2 and lactate dehydrogenase A was an independent poor prognostic marker for survival. PMID: 26989901
  41. Although lactate dehydrogenase A (LDHA) expression varies biphasically during melanoma brain metastasis formation, tumor progression and survival seem to be functionally independent of LDHA. PMID: 25791837
  42. dehydrogenase A negatively regulated by miRNAs promotes aerobic glycolysis and is increased in colorectal cancer. PMID: 26062441
  43. positive relationship between LDH-A expression and CSC/EMT markers was confirmed both in invasive bladder cell line and in 136 MIBC specimens. PMID: 26721441
  44. FOXM1-LDHA signaling functioned as a stimulator of glycolysis and promoted gastric cancer progression. PMID: 26261559
  45. Mammalian photoreceptors contain dimeric and tetrameric PKM2 and LDH-A. This is consistent with the ability to switch between energy production and biosynthesis like a proliferating tissue, possibly due to demands of opsin synthesis. PMID: 26780311
  46. We propose a profibrotic feed forward loop, in which radiation induces LDHA expression and lactate production, which can lead to further activation of TGF-beta to drive the fibrotic process. PMID: 26254422
  47. These results indicate that disrupting SLC2A1, LDHA, or other regulators in cancer cell energetics is a very promising approach for new targeted therapies. PMID: 25838393
  48. High expression of LDHA is associated with gastric cancer. PMID: 25913622
  49. High LDHA expression is associated with tumor progression in prostate cancer. PMID: 25983002
  50. LDHA is downregulated by JQ1, which suppresses tumor growth in ovarian cancer PMID: 25762632
  51. Our data shows that overexpression and increased phosphorylation of PKM2 and LHDA is a common finding in thyroid malignancies. PMID: 25880801
  52. The structures presented here provide insights into LDHA enzyme mechanism and inhibition and a framework for structure-assisted drug design that may contribute to new cancer therapies. PMID: 25664730
  53. LDHA overexpression is correlated with disease stage and pancreatic cancer progression. PMID: 24947925
  54. LDHA expression is elevated in gastric cancer cells and antagonism with oxamic acid inhibited cell proliferation and migration. PMID: 25394466
  55. Inhibition of lactate dehydrogenase A by microRNA-34a resensitizes colon cancer cells to 5-fluorouracil. PMID: 25333573
  56. LDHA crystal structure shows that NADH binding induces small conformational changes in the active-site loop and an adjacent helix. PMID: 24816116
  57. LDHA was silenced in IDHmt derived brain tumor stem cells. Silencing of LDHA was associated with increased methylation of the LDHA promoter. first demonstration of downregulation of LDHA in cancer. PMID: 24366912
  58. In this study, we inhibited LDH using oxamate resulting in the inhibition of proliferation and induction of apoptosis in gastric cancer PMID: 25524555
  59. lactate dehydrogenase A is expressed in lung adenocarcinomas and may predict response to anti-LDHA therapy in lung adenocarcinomas using 18F-FDG PET/CT PMID: 25342384
  60. LDH-A expression is an independent prognostic risk factor in gastric cancer patients. PMID: 24608789
  61. Data indicate that that transcription factor Forkhead box protein M1 (FOXM1) bound directly to the lactate dehydrogenase A (LDHA) promoter region and regulated the expression of the LDHA gene at the transcriptional level. PMID: 24634381
  62. LDHA up-regulation can be a predictor of poor prognosis in clear cell renal cell carcinoma. PMID: 24885701
  63. Serum LDH levels may be a predictive marker of hypersensitivity reactions in patients treated with L-OHP. Further extensive examinations with a larger number of patients are needed to establish a patient management strategy. PMID: 24782655
  64. the activity and expression of LDHA are upregulated in doxorubicin-resistant cells. Moreover, our data showed a strong correlation between glucose metabolism and doxorubicin resistance in chondrosarcoma cells PMID: 24789077
  65. We demonstrate that lactate dehydrogenase (LDH) isoform 5 secreted by glioblastoma cells induces NKG2D ligands on monocytes isolated from healthy individuals. PMID: 25136121
  66. The results of this meta-analysis suggest that high LDH5 expression is associated with HIF-1alpha and poor overall survival in cancer patients. PMID: 24744144
  67. LDH5 expression may play a role in the regulation of tumoral and stromal vascular endothelial growth factor expression in gastric cancer PMID: 24401755
  68. Overexpression of lactate dehydrogenase-A is associated with intrahepatic cholangiocarcinoma. PMID: 24679073
  69. this study investigated the role of LDHA gene in prostate cancer radioresistance. PMID: 24714743
  70. It is shown that shRNA mediated knockdown of lactate dehydrogenase A (LDHA), a key mediator of aerobic glycolysis, results in elevated mitochondrial ROS production and a concomitant decrease in cell proliferation and motility. PMID: 23583676
  71. Pharmacologic inhibition of lactate dehydrogenase-A suppressed the conversion of hyperpolarized (13)C-pyruvate to lactate. PMID: 23722553
  72. DC-SIGNR has a role in expression of lactic acid dehydrogenase and beta2-microglobulin in non-Hodgkin lymphoma PMID: 23859015
  73. Human LDHA was introduced as a transgene to Ldhc-null mice and rescued sperm function. PMID: 23467744
  74. LDH inhibition in Burkitt lymphoma cells led to decreased NAD cellular levels, which resulted in sirtuin-1 inhibition and MYC down-regulation. PMID: 23797802
  75. the role played by ERRalpha in the regulation of lactate dehydrogenases A and B PMID: 23516535
  76. Lactate dehydrogenase A is overexpressed in pancreatic cancer. PMID: 23404405
  77. Data indicate that serum lactic dehydrogenase (S-LDH) appears to be a significant independent prognostic index in patients with metastatic nasopharyngeal carcinoma (NPC). PMID: 23266049
  78. Increased LDH5 expression is associated with lymph node metastasis in oral squamous cell carcinoma. PMID: 23184277
  79. LDHA plays an important role in the progression of esophageal squamous cell carcinoma by modulating cell growth PMID: 22961700
  80. Elevated lactate dehydrogenase level is associated with recurrent or refractory aggressive lymphoma. PMID: 23166385
  81. Studies indicate the mechanisms by which lactate dehydrogenase A (LDHA) promotes tumor growth and metastasis. PMID: 22897481
  82. Cells expressing either PDK1 or LDHA maintained a lower mitochondrial membrane potential and decreased reactive oxygen species production with or without exposure to toxins. PMID: 22948140
  83. Lactic acid induces myofibroblast differentiation via pH-dependent activation of transforming growth factor beta. PMID: 22923663
  84. Data suggest that serum lactate dehydrogenase (LDH) kinetics might reflect disease behaviour in extracranial metastatic and primary sites without need for comprehensive imaging studies and is a quite inexpensive diagnostic test. PMID: 22593701
  85. We demonstrate that LDH-A reduction can suppress the tumorigenicity of intestinal-type gastric cancer (ITGC) cells by downregulating Oct4 both in vitro and in vivo. PMID: 22429998
  86. LDH-A reduction resulted in an inhibited cancer cell proliferation, elevated intracellular oxidative stress, and induction of mitochondrial pathway apoptosis. PMID: 21452021
  87. A protein encoded by this locus was found to be differentially expressed in postmortem brains from patients with atypical frontotemporal lobar degeneration. PMID: 22360420
  88. Results show that S-100B, MIA and LDH levels were significantly higher in patients with advanced melanoma than in disease-free patients or healthy controls. PMID: 21858537
  89. LDH-A is tyrosine phosphorylated and activated by FGFR1 in cancer cells. PMID: 21969607
  90. Case Report: describe an elderly patient with physical therapy-induced rhabdomyolysis complicated by acute kidney injury associated with reduced skeletal muscle LDH-A activity. PMID: 22127970
  91. Serum LDH and tissue LDH5 levels are complementary features that help to characterize the activity of LDH in colorectal cancer and have a potent value in predicting response to chemotherapy. PMID: 21632858
  92. High LDH is associated with M1b prostate cancer. PMID: 21249322
  93. LDH5 is overexpressed in non-small cell lung cancer and could serve as a marker for malignancy. LDH5 correlates positively with the prognostic marker transketolase like 1 protein. PMID: 20385008
  94. High lactate dehydrogenase is associated with acute adult T-cell leukemia/lymphoma. PMID: 20828817
  95. Presented are QM/MM calculations that show differences in geometries of active sites of M(4) and H(4) isoforms of human LDH ligated with oxamate, pyruvate or L-lactate. PMID: 20951115
  96. Correlation of LDH-5 expression with clinicopathological factors and with the expression of Bcl-2, Bcl-XL, Mcl-1 and GRP78 was examined in pigmented lesions, including nevi and melanoma at different stages of progression PMID: 19838163
  97. an LDHA exon5 haplotype confers increased risk for paradoxically decreased minute volume respiratory response to CO2 challenge but not to panic disorder PMID: 12555229
  98. The study of this protein in a sportsman is significant for assessment of training efficiency. PMID: 12629811
  99. The activity of this enzyme was studied in tissues, erythrocytes, and blood plasma of patients with peptic ulcer both in its uncomplicated course and in the development of complications. PMID: 12712614
  100. These data indicate that LDH-A is induced through a non-genomic pathway of estrogen action. PMID: 15240094

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Involvement in disease Glycogen storage disease 11 (GSD11)
Subcellular Location Cytoplasm
Protein Families LDH/MDH superfamily, LDH family
Database Links

HGNC: 6535

OMIM: 150000

KEGG: hsa:3939

STRING: 9606.ENSP00000445175

UniGene: Hs.2795

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