Product Details

Target Name

left-right determination factor 2

Alternative Names

EBAF ELISA Kit; Endometrial bleeding associated factor (left right determination factor A transforming growth factor beta superfamily) ELISA Kit; Endometrial bleeding associated factor ELISA Kit; Endometrial bleeding-associated factor ELISA Kit; Left right determination factor 2 ELISA Kit; Left right determination factor A ELISA Kit; Left-right determination factor 2 ELISA Kit; Left-right determination factor A ELISA Kit; LEFTA ELISA Kit; LEFTY 2 ELISA Kit; LEFTY2 ELISA Kit; LEFTYA ELISA Kit; LFTY2 transforming growth factor; beta -4 ELISA Kit; LFTY2; mouse; homolog of ELISA Kit; LFTY2_HUMAN ELISA Kit; MGC46222 ELISA Kit; Protein lefty 2 ELISA Kit; Protein lefty A ELISA Kit; Protein lefty-2 ELISA Kit; Protein lefty-A ELISA Kit; Protein lefty2 ELISA Kit; PSEC0024 ELISA Kit; TGF beta 4 ELISA Kit; TGF-beta-4 ELISA Kit; TGFB4 ELISA Kit; Transforming growth factor beta 4 ELISA Kit; Transforming growth factor beta-4 ELISA Kit

Abbreviation

LEFTY2

Uniprot No.

O00292

Species

Homo sapiens (Human)

Sample Types

serum, plasma

Detection Range

7.8 pg/mL-500 pg/mL

Sensitivity

1.95 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Cancer

Assay Principle

quantitative

Measurement

Sandwich

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human LEFTY2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	91
	Range %	86-95
1:200	Average %	102
	Range %	97-107
1:400	Average %	91
	Range %	85-97
1:800	Average %	97
	Range %	91-103

Recovery

The recovery of human LEFTY2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-98
EDTA plasma (n=4)	97	90-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
500	2.811	2.836	2.824	2.744
250	2.038	2.000	2.019	1.939
125	1.331	1.368	1.350	1.270
62.5	0.804	0.752	0.778	0.698
31.2	0.473	0.498	0.486	0.406
15.6	0.278	0.281	0.280	0.200
7.8	0.173	0.169	0.171	0.091
0	0.079	0.081	0.080

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

Description

This Human LEFTY2 ELISA Kit was designed for the quantitative measurement of Human LEFTY2 protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 7.8 pg/mL-500 pg/mL and the sensitivity is 1.95 pg/mL.

Code	CSB-E13590h
Size	96T,5×96T,10×96T
Price	Request a Quote or Start an on-line Chat
Trial Size	24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Human left-right determination factor 2(LEFTY2) ELISA Kit

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