Human myelin-associated glycoprotein(MAG) ELISA Kit

Code CSB-E17901h
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P20916
Lead Time 3-5 working days
Abbreviation MAG
Protein Biological Process 1 Cell Adhesion
Target Name myelin associated glycoprotein
Alias GMA, S-MAG, SIGLEC-4A, SIGLEC4A, myelin-associated glycoprotein|sialic acid-binding immunoglobulin-like lectin 4A
Species Homo sapiens (Human)
Protein Biological Process 3 Cell adhesion
Sample Types serum, plasma, cerebrospinal fluid (CSF)
Detection Range 0.625 ng/mL-40 ng/mL
Sensitivity 0.156 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Neuroscience
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This protein is a type I membrane protein and member of the immunoglobulin superfamily. It is thought to be involved in the process of myelination. It is a lectin that binds to sialylated glycoconjugates and mediates certain myelin-neuron cell-cell interactions. Two alternatively spliced transcripts encoding different isoforms have been described for this gene.
HGNC 28157
RGD 1565703
MGI 3603816
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human MAG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %98
Range %89-101
1:2Average %92
Range %84-96
1:4Average %90
Range %85-95
1:8Average %98
Range %90-102
Recovery
The recovery of human MAG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9789-101
EDTA plasma (n=4)9890-102
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
401.754 1.721 1.738 1.615
201.395 1.422 1.409 1.286
100.872 0.911 0.892 0.769
50.551 0.555 0.553 0.430
2.50.365 0.348 0.357 0.234
1.250.231 0.242 0.237 0.114
0.6250.187 0.184 0.186 0.063
00.124 0.122 0.123
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Function Adhesion molecule that mediates interactions between myelinating cells and neurons by binding to neuronal sialic acid-containing gangliosides and to the glycoproteins RTN4R and RTN4RL2 (By similarity). Not required for initial myelination, but seems to play a role in the maintenance of normal axon myelination. Protects motoneurons against apoptosis, also after injury; protection against apoptosis is probably mediated via interaction with neuronal RTN4R and RTN4RL2. Required to prevent degeneration of myelinated axons in adults; this probably depends on binding to gangliosides on the axon cell membrane (By similarity). Negative regulator of neurite outgrowth; in dorsal root ganglion neurons the inhibition is mediated primarily via binding to neuronal RTN4R or RTN4RL2 and to a lesser degree via binding to neuronal gangliosides. In cerebellar granule cells the inhibition is mediated primarily via binding to neuronal gangliosides. In sensory neurons, inhibition of neurite extension depends only partially on RTN4R, RTN4RL2 and gangliosides. Inhibits axon longitudinal growth (By similarity). Inhibits axon outgrowth by binding to RTN4R (By similarity). Preferentially binds to alpha-2,3-linked sialic acid. Binds ganglioside Gt1b (By similarity).
Involvement in disease Spastic paraplegia 75, autosomal recessive (SPG75)
Subcellular Location Cell membrane, Single-pass type I membrane protein, Membrane raft
Protein Families Immunoglobulin superfamily, SIGLEC (sialic acid binding Ig-like lectin) family
Tissue Specificity Both isoform 1 and isoform 2 are detected in myelinated structures in the central and peripheral nervous system, in periaxonal myelin and at Schmidt-Lanterman incisures (PubMed:9495552, PubMed:6200494). Detected in optic nerve, in oligodendroglia and in p
Database Links

HGNC: 6783

OMIM: 159460

KEGG: hsa:4099

STRING: 9606.ENSP00000376048

UniGene: Hs.643440

Pathway Inhibitors of axonal regeneration

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