Human soluble cluster of differentiation 14,sCD14 ELISA Kit

Code CSB-E13199h
See More Details
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P08571
Lead Time 3-5 working days
Abbreviation CD14
Protein Biological Process 1 others
Target Name CD14 molecule
Alias , CD14 antigen|monocyte differentiation antigen CD14
Species Homo sapiens (Human)
Protein Biological Process 3 Immunity
Sample Types serum, plasma, cell culture supernates, tissue homogenates, urine
Detection Range 0.234 ng/mL-15 ng/mL
Sensitivity 0.229 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details CD14 is a surface protein preferentially expressed on monocytes/macrophages. It binds lipopolysaccharide binding protein and recently has been shown to bind apoptotic cells. Alternative splicing results in multiple transcript variants encoding the same isoform.
HGNC 1628
RGD 620588
MGI 88318
Precision

 

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays):CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

 

Linearity

 

To assess the linearity of the assay, samples were spiked with high concentrations of human sCD14 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

 

Typical Data

 

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

 

References
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Function Coreceptor for bacterial lipopolysaccharide
Subcellular Location Cell membrane, Lipid-anchor, GPI-anchor, Secreted, Membrane raft, Golgi apparatus
Tissue Specificity Detected on macrophages (at protein level) (PubMed:1698311). Expressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages.
Database Links

HGNC: 1628

OMIM: 158120

KEGG: hsa:929

STRING: 9606.ENSP00000304236

UniGene: Hs.163867

Pathway MAPK signaling pathway
NF-kappa B signaling pathway
Regulation of actin cytoskeleton
Hematopoietic cell lineage
Toll-like receptor signaling pathway

Q&A and Customer Reviews

Q&A
Q:

For item CSB-E13199h,if the kit detects both soluble and membrane bound CD14. Would you have any testing data that addresses this question that you could share?

A:
Thanks for your inquiry.
CD14 exists in two forms, one anchored to the membrane by a glycosylphosphatidylinositol tail (mCD14), the other a soluble form (sCD14).
Soluble CD14 either appears after shedding of mCD14 or is directly secreted from intracellular vesicles.
CSB-E13199h This kit is designed according to: https://www.uniprot.org/uniprot/P08571. It detects soluble form.
For serum and plasma samples, CD14 usually exists in soluble form and the kit detects soluble CD14.
For cell samples, after cellwall breaking, the hydrophobic region of the membrane protein will be closed, and the soluble component can be detected at this time.
We tested serum and plasma samples before and the test value is 3.75--7.5ng/ml under 1:200 dilution.
The final concentration is 750--1500ng/ml.
Pls let me know if you have any further questions. Thank you.
Customer Reviews

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