Human soluble tumor necrosis factor-like weak inducer of apoptosis (sTWEAK)ELISA Kit

Instructions
Code CSB-E16492h
Size 96T,5×96T,10×96T

Product Details

Target Name tumor necrosis factor (ligand) superfamily, member 12
Alternative Names APO3L, DR3LG, MGC129581, MGC20669, TWEAK, APO3/DR3 ligand|TNF-related WEAK inducer of apoptosis
Abbreviation TNFSF12
Uniprot No. O43508
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.312 ng/ml-20 ng/ml
Sensitivity 0.078 ng/ml
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human sTWEAK in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 87  
Range % 80-98  
1:2 Average % 95  
Range % 92-104  
1:4 Average % 94  
Range % 89-100  
1:8 Average % 92  
Range % 85-100  
Recovery
The recovery of human sTWEAK spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 87 82-100  
EDTA plasma (n=4) 92 90-96  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
2000 2.501 2.495 2.498 2.336  
1000 1.862 1.884 1.873 1.711  
500 1.174 1.172 1.173 1.011  
250 0.702 0.687 0.695 0.533  
125 0.422 0.402 0.412 0.250  
62.5 0.310 0.291 0.301 0.139  
31.25 0.207 0.221 0.214 0.052  
0 0.159 0.165 0.162    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days
Still Have Questions? Leave a Message or Start an on-line Chat

Target Data

Function Binds to FN14 and possibly also to TNRFSF12/APO3. Weak inducer of apoptosis in some cell types. Mediates NF-kappa-B activation. Promotes angiogenesis and the proliferation of endothelial cells. Also involved in induction of inflammatory cytokines. Promotes IL8 secretion.
Subcellular Location Cell membrane, Single-pass type II membrane protein, SUBCELLULAR LOCATION: Tumor necrosis factor ligand superfamily member 12, secreted form: Secreted, SUBCELLULAR LOCATION: Isoform TWE-PRIL: Cell membrane, Single-pass membrane protein
Protein Families Tumor necrosis factor family
Tissue Specificity Highly expressed in adult heart, pancreas, skeletal muscle, brain, colon, small intestine, lung, ovary, prostate, spleen, lymph node, appendix and peripheral blood lymphocytes. Low expression in kidney, testis, liver, placenta, thymus and bone marrow. Als
Database Links

HGNC: 11927

OMIM: 602695

KEGG: hsa:407977

UniGene: Hs.54673

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