Human soluble tumor necrosis factor-like weak inducer of apoptosis (sTWEAK)ELISA Kit

Code CSB-E16492h
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. O43508
Lead Time 3-5 working days
Abbreviation TNFSF12
Protein Biological Process 1 Angiogenesis
Target Name tumor necrosis factor (ligand) superfamily, member 12
Alias APO3L, DR3LG, MGC129581, MGC20669, TWEAK, APO3/DR3 ligand|TNF-related WEAK inducer of apoptosis
Species Homo sapiens (Human)
Protein Biological Process 3 Angiogenesis
Sample Types serum, plasma, tissue homogenates
Detection Range 0.312 ng/ml-20 ng/ml
Sensitivity 0.078 ng/ml
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This protein is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This protein is a ligand for the FN14/TWEAKR receptor. This cytokine has overlapping signaling functions with TNF, but displays a much wider tissue distribution. This cytokine can induce apoptosis via multiple pathways of cell death in a cell type-specific manner. This cytokine is also found to promote proliferation and migration of endothelial cells, and thus acts as a regulator of angiogenesis. Some transcripts that skip the last exon of this gene and continue with the second exon of the neighboring TNFSF13 gene have been identified; such read-through transcripts are contained in GeneID 407977, TNFSF12-TNFSF13.
HGNC 11927
RGD 1359620
MGI 1196259
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human sTWEAK in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 87  
Range % 80-98  
1:2 Average % 95  
Range % 92-104  
1:4 Average % 94  
Range % 89-100  
1:8 Average % 92  
Range % 85-100  
Recovery
The recovery of human sTWEAK spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 87 82-100  
EDTA plasma (n=4) 92 90-96  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
2000 2.501 2.495 2.498 2.336  
1000 1.862 1.884 1.873 1.711  
500 1.174 1.172 1.173 1.011  
250 0.702 0.687 0.695 0.533  
125 0.422 0.402 0.412 0.250  
62.5 0.310 0.291 0.301 0.139  
31.25 0.207 0.221 0.214 0.052  
0 0.159 0.165 0.162    
References
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Function Binds to FN14 and possibly also to TNRFSF12/APO3. Weak inducer of apoptosis in some cell types. Mediates NF-kappa-B activation. Promotes angiogenesis and the proliferation of endothelial cells. Also involved in induction of inflammatory cytokines. Promotes IL8 secretion.
Subcellular Location Cell membrane, Single-pass type II membrane protein, SUBCELLULAR LOCATION: Tumor necrosis factor ligand superfamily member 12, secreted form: Secreted, SUBCELLULAR LOCATION: Isoform TWE-PRIL: Cell membrane, Single-pass membrane protein
Protein Families Tumor necrosis factor family
Tissue Specificity Highly expressed in adult heart, pancreas, skeletal muscle, brain, colon, small intestine, lung, ovary, prostate, spleen, lymph node, appendix and peripheral blood lymphocytes. Low expression in kidney, testis, liver, placenta, thymus and bone marrow. Als
Database Links

HGNC: 11927

OMIM: 602695

KEGG: hsa:407977

UniGene: Hs.54673

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