Purity
Greater than 95% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/ug as determined by LAL method.
Activity
①Measured by its binding ability in a functional ELISA. Immobilized Human TNFSF12 at 2 μg/mL can bind Human TNFRSF12A (CSB-MP873605HU). The EC50 is 0.7706-0.9123 ng/mL.
②Measured by its binding ability in a functional ELISA. Immobilized Human TNFSF12 at 2 μg/mL can bind Anti-TNFSF12 recombinant antibody (CSB-RA023987MA1HU). The EC50 is 2.862-3.195 ng/mL.
③Human TNFRSF12A (CSB-MP873605HU) captured on Protein A Chip can bind Recombinant Human TNFSF12 with an affinity constant of 0.154 nM as detected by MetaSPR Assay (WeSPRTM 200).
④TNFSF12 Recombinant Monoclonal Antibody (CSB-RA023987MA1HU) captured on Protein A Chip can bind Recombinant Human TNFSF12 with an affinity constant of 0.205 nM as detected by MetaSPR Assay (WeSPRTM 200).
Alternative Names
Tumor necrosis factor ligand superfamily member 12; APO3 ligand; TNF-related weak inducer of apoptosis; TWEAK [Cleaved into: Tumor necrosis factor ligand superfamily member 12, membrane form; Tumor necrosis factor ligand superfamily member 12, secreted form]
Species
Homo sapiens (Human)
Expression Region
94-249aa
Target Protein
Sequence
SAPKGRKTRARRAIAAHYEVHPRPGQDGAQAGVDGTVSGWEEARINSSSPLRYNRQIGEFIVTRAGLYYLYCQVHFDEGKAVYLKLDLLVDGVLALRCLEEFSATAASSLGPQLRLCQVSGLLALRPGSSLRIRTLPWAHLKAAPFLTYFGLFQVH
Tag Info
N-terminal 6xHis-Myc-tagged
Form
Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid
repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage
temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Datasheet & COA
Please contact us to get it.
