Human transferrin(TF) ELISA Kit

Code CSB-E13093h
Size 96T,5×96T,10×96T
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Product Details

Alternative Names
Apotransferrin ELISA Kit; Beta 1 metal binding globulin ELISA Kit; Beta-1 metal-binding globulin ELISA Kit; DKFZp781D0156 ELISA Kit; PRO1400 ELISA Kit; PRO1557 ELISA Kit; PRO2086 ELISA Kit; Serotransferrin ELISA Kit; Serotransferrin precursor ELISA Kit; Siderophilin ELISA Kit; TF ELISA Kit; TFQTL1 ELISA Kit; Transferin ELISA Kit; Transferrin ELISA Kit; TRFE_HUMAN ELISA Kit
Abbreviation
TF
Uniprot No.
Species
Homo sapiens (Human)
Sample Types
serum, plasma, urine, cell culture supernates, cerebrospinal fluid (CSF)
Detection Range
0.023 nmol/L-1.5 nmol/L
Sensitivity
0.006 nmol/L
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cardiovascular
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human TF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:50000 Average % 89  
Range % 84-95  
1:100000 Average % 92  
Range % 87-97  
1:200000 Average % 96  
Range % 90-101  
1:400000 Average % 90  
Range % 86-96  
Recovery
The recovery of human TF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 96 89-101  
EDTA plasma (n=4) 98 93-105  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
nmol/L OD1 OD2 Average Corrected  
1.5 2.367 2.396 2.382 2.278  
0.75 1.898 1.753 1.826 1.722  
0.375 1.262 1.288 1.275 1.171  
0.187 0.948 0.982 0.965 0.861  
0.094 0.583 0.597 0.590 0.486  
0.047 0.353 0.348 0.351 0.247  
0.023 0.284 0.295 0.290 0.186  
0 0.102 0.106 0.104    
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human transferrin (TF) ELISA Kit was designed for the quantitative measurement of Human transferrin (TF) protein in serum, plasma, urine, cell culture supernates, cerebrospinal fluid (CSF). It is a Sandwich ELISA kit, its detection range is 0.023 nmol/L-1.5 nmol/L and the sensitivity is 0.006 nmol/L.

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Target Background

Function
(From Uniprot)
Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation.; (Microbial infection) Serves as an iron source for Neisseria species, which capture the protein and extract its iron for their own use.
Gene References into Functions
  1. High transferrin expression is associated with Spontaneous intracranial hypotension. PMID: 29621631
  2. rs4525863, rs1880669, and rs2692695 SNPs of the TF gene might be a protective factor for ischemic stroke in Southern Chinese Han population. PMID: 29936663
  3. our data establish that blood TSAT exerts a critical role in experimental stroke-induced brain damage. In addition, our findings suggest that the protective effect of iron-free transferrin (apotransferrin, ATf) at the neuronal level resides in preventing NMDA-induced HTf uptake and ROS production, which in turn reduces neuronal damage PMID: 29248829
  4. Histochemical staining with anti-Tf antibody and an N-acetylglucosamine-binding lectin suggests that brain-type Tf is secreted from choroid plexus, CSF-producing tissue PMID: 29701803
  5. Data suggest that serum levels of biomarkers of iron status (ferritin, transferrin, and hemoglobin) are positively associated with risk of hyperuricemia in Chinese adults. PMID: 29425155
  6. Transfeerrin saturation was associated with less severe anemia in early CKD patients. PMID: 29227972
  7. Three single nucleotide polymorphisms associated with iron regulation were genotyped in multiple sclerosis : two in the human hereditary hemochromatosis protein gene HFE: rs1800562 (C282Y mutation) and rs1799945 (H63D mutation), as well as the rs1049296 SNP in the transferrin gene (C2 mutation). We only observed a higher prevalence of TF-C2 in multiple sclerosis patients PMID: 29201641
  8. Increased serum transferrin and ceruloplasmin turnover in diet-controlled patients with type 2 diabetes has been reported. PMID: 29079528
  9. model predicts that small vesicles promote activation of FX by the extrinsic tenase (VIIa/TF) significantly better than large vesicles PMID: 28935233
  10. This work presents an HPLC analysis of carbohydrate-deficient transferrin. PMID: 28971232
  11. Transferrin associated with Adenoid Cystic Carcinoma of Parotid Gland in immunohistochemical study, was found up-regulated in Pleomorphic Adenoma of the Parotid Gland. PMID: 28497265
  12. The RNAi knock-down of Trypanosoma brucei GSK3beta reduced Tf endocytosis. PMID: 27626104
  13. holotransferrin treatment to recombinant-TfR1 HepG2 cells did not elevate HAMP responses compared to untreated or wild-type cells. In PMID: 27667164
  14. Blood lead levels were positively associated with plasma homocysteine levels measured 4-6 years later, and three SNPs in the TF gene modified the association. PMID: 28775131
  15. In this technical note, the aberrant glycosylation profiles of transferrin glycoforms in congenital disorder of glycosylation patients are presented to shed light on the Mass Spectrometry of native transferrin and glycopeptides from the viewpoint of clinical glycoproteomics. PMID: 27095603
  16. Over the first 500 s, approximately 92,000 molecules of thrombin were generated per surface TF molecule for the 250-mum-long coating. PMID: 27605669
  17. High carbohydrate deficient transferrin is associated with relapse in alcohol-dependent patients. PMID: 26851349
  18. High Plasma Carbohydrate-deficient transferrin is associated with monoclonal light chain gammopathy. PMID: 27003373
  19. the value of transferrin receptors (TfRs)/cell reveals a 100-fold increase in the number of TfRs per bEnd3 cells compared to human umbilical vein cells. PMID: 27100851
  20. During pregnancy, the levels of 2-, 5- and 6-sialo transferrin isoforms were increasing while 3- and 4-sialo transferrin isoforms were decreasing. PMID: 27448041
  21. alterations in transferrin glycosylation/sialylation in pancreatic cancer differ from the alterations in some other cancers. PMID: 27312554
  22. In a multiple regression analysis, FN3K rs1056534, TF polymorphism and presence of diabetes mellitus were predictors for HHV-8 infection. PMID: 27461879
  23. the analysis of the hTf complex with a bacterial receptor that has evolved to sequester iron identifies two regions contacting rapidly evolving residues that mechanically manipulate dissociation from the pathogen PMID: 26955866
  24. Transferrin mutations can severely impair the diagnostics of chronic alcohol abuse. PMID: 26333807
  25. Total iron binding capacity was strongly associated with variants in and near the TF gene, and with variants in HFE gene in premenopausal women of European descent. PMID: 26852655
  26. Data suggest that up-regulation of serum transferrin levels is positively associated with risk of metabolic syndrome and overweight/obesity, but not with risk of diabetes among Chinese men and women. PMID: 25914390
  27. activation of PI3K/Akt by retinoic acid modulates AMPK activity in ECs and plays a crucial role in the inhibition of coagulatory factors such as TF, PAI-1, and HMGB1 in inflammatory conditions PMID: 26116962
  28. TF severed as the carrier to delivery irons, and could directly stimulate cardiomyocytes hypertrophy. PMID: 26099594
  29. Data show that transferrin (Tf) was attached to nanoparticles with an acid-cleavable linkage that facilities release of nanoparticles from Tf that are bound to Tf receptors (TfR) during transcytosis, promoting entry of the nanoparticles into the brain. PMID: 26392563
  30. H2O2 induces the expression of transferrin, and consequently, decreased iron absorption, suggests a novel mechanism for iron deficiency in pediatric non-alcoholic steatohepatitis patients. PMID: 25000850
  31. Human transferrin is internalized across the mycobacterial cell wall in a GAPDH-dependent manner within infected macrophages. PMID: 25163484
  32. Data suggest that the 15 disulfide bonds in transferrin vary in their susceptibility to reduction and in their contribution to protein conformation/stability. PMID: 25716754
  33. apelin-13 has a role in inducing expression of prothrombotic tissue factor PMID: 25298206
  34. identified the rs3811647 polymorphism in the TF gene as the only SNP significantly associated with iron metabolism through serum transferrin and iron levels PMID: 25457201
  35. significant associations between serum iron binding capacity levels and two SNPs around TF on chromosome 3 were identified. PMID: 25224454
  36. Hereditary hypotransferrinemia caused by novel transferrin mutations can lead to elevated transferrin saturation and, when associated with HFE or HAMP mutations, to iron overload. PMID: 25486930
  37. Results show that higher TF expression in patients with hypereosinophilic disorders may contribute to increase the thrombotic risk. PMID: 25375118
  38. genetic association studies in a population of black women in South Africa: Data confirm that an SNP in TF (rs1799852) is associated with iron status/iron-deficiency anemia in the population studied. PMID: 25809685
  39. decreased circulating levels in abdominal aortic aneurysm PMID: 24599423
  40. Infants born to mothers with the HFE C282Y gene missense variant but not the TF P570S or HFE H63D gene missense variants had lower umbilical cord blood lead levels relative to those born to women who were wild-type. PMID: 25287020
  41. TF translocates to the cell front in association with cytoskeleton proteins and regulates HVSMC migration by mechanisms dependent and independent of factor (F)VIIa/PAR2 PMID: 22938499
  42. did not find any association between the c.-2G>A polymorphism and keratoconus; no association was found between transferrin polymorphisms and Fuchs endothelial corneal dystrophy occurrence PMID: 24350254
  43. Understanding the interaction between [Cr(phen)3](3+) with transferrin is relevant because this protein could be a delivery agent of Cr(III) complex to tumor cells. PMID: 24972167
  44. Increased urinary excretion of plasma proteins such as IgG, ceruloplasmin and transferrin, with different molecular radii of 55 A or less and different isoelectric points precede development of microalbuminuria in patients with NIDDM and hypertension. PMID: 24256706
  45. Elevated BMI reduces the diagnostic utility of carbohydrate-deficient transferrin at higher alcohol intake in subjects with liver disease. PMID: 23875541
  46. Data suggest that there is no association between plasma level of carbohydrate-deficient transferrin and volume decrease of brain gray or white matter in male subjects as result of current/recent or lifetime alcohol drinking in the Netherlands. PMID: 23027680
  47. Studies indicate that several genes have been linked to iron homeostasis, including transferrin (TF), iron regulatory protein 1 (ACO1) and transferrin receptor 2 (TFR2). PMID: 23817740
  48. The A allele of SNP rs3811647 increases Tf expression in a manner that might underlie inter-individual variation in serum transferrin levels PMID: 23588470
  49. Transferrin mutations were found in several members of 2 non-consanguineous families with hypochromic microcytic anemia & hemodiserosis. The missense mutations were 1940 A>T & 1825 C>T. PMID: 23888904
  50. The structures of hTF observed here represent key conformers captured in the dynamic nature of the transferrin family proteins and provide a structural basis for understanding the mechanism of metal uptake and release in transferrin families. PMID: 23256035

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Involvement in disease
Atransferrinemia (ATRAF)
Subcellular Location
Secreted.
Protein Families
Transferrin family
Tissue Specificity
Expressed by the liver and secreted in plasma.
Database Links

HGNC: 11740

OMIM: 190000

KEGG: hsa:7018

STRING: 9606.ENSP00000385834

UniGene: Hs.518267

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