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TF Recombinant Monoclonal Antibody

Datasheet
Code CSB-RA023412MA1HU
Size US$210
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Image
  • Western Blot
    Positive WB detected in: HEK293 whole cell lysate(30µg)), Hela whole cell lysate(30µg)
    All lanes: TF antibody at 1:1000
    Secondary
    Goat polyclonal to human IgG at 1/40000 dilution
    Predicted band size: 77 kDa
    Observed band size: 77 kDa
    Exposure time: 5min
  • IHC image of CSB-RA023412MA1HU diluted at 1:50 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Anti-Human lgG, Fcy Fragment Specific labeled by HRP and visualized using 0.05% DAB.
  • Overlay Peak curve showing HepG2 cells stained with CSB-RA023412MA1HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was Fluorescein (FITC) AffiniPure Goat Anti-Human IgG, Fcγ fragment specific at 1:200 dilution for 35 min at 4℃.Control antibody (green line) was human IgG1 (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
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Product Details

Uniprot No.
P02787
Target Names
TF
Alternative Names
Serotransferrin (Transferrin) (Beta-1 metal-binding globulin) (Siderophilin), TF
Species Reactivity
Human
Immunogen
Recombinant Human TF protein
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated
Clonality
Monoclonal
Isotype
hIgG1
Clone No.
13D2
Purification Method
Affinity-chromatography
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form
Liquid
Tested Applications
ELISA, WB, IHC, FC
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:2000
IHC 1:50-1:200
FC 1:50-1:200
Protocols
ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Flow Cytometry (FC) Protocol
Troubleshooting and FAQs
Antibody FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Description

Transferrin plays a central role in iron homeostasis, functioning as the primary plasma protein responsible for ferric iron transport throughout the body. This serotransferrin, also known as beta-1 metal-binding globulin or siderophilin, delivers iron to cells via receptor-mediated endocytosis and serves as a critical marker in studies of iron metabolism, anemia, liver function, and cellular proliferation. Researchers investigating iron-dependent processes, from erythropoiesis to tumor biology, rely on precise detection of this 77 kDa glycoprotein.

This recombinant monoclonal antibody against human transferrin offers the reproducibility that demanding experimental workflows require. Generated through recombinant technology with a defined sequence, clone 13D2 eliminates the lot-to-lot variability inherent in traditional hybridoma production. The human IgG1 isotype format and affinity chromatography purification ensure consistent performance across extended studies and multi-site collaborations.

Validation across multiple platforms demonstrates genuine experimental flexibility. Western blot analysis confirms specific detection at the expected 77 kDa molecular weight in both HEK293 and HeLa whole cell lysates, with optimal performance at dilutions between 1:500 and 1:2000. Immunohistochemistry on paraffin-embedded human liver tissue reveals clear staining patterns using citrate buffer antigen retrieval, supporting applications in tissue-based research. Flow cytometry analysis of HepG2 cells shows distinct positive population shifts compared to isotype controls, enabling quantitative assessment of transferrin expression at the single-cell level.

This antibody serves researchers exploring iron transport mechanisms, hepatocyte biology, metabolic disorders, and cancer metabolism where transferrin expression patterns provide meaningful biological insights.

Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Customer Reviews and Q&A

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Target Background

Function
Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation.; (Microbial infection) Serves as an iron source for Neisseria species, which capture the protein and extract its iron for their own use.
Gene References into Functions
  1. High transferrin expression is associated with Spontaneous intracranial hypotension. PMID: 29621631
  2. rs4525863, rs1880669, and rs2692695 SNPs of the TF gene might be a protective factor for ischemic stroke in Southern Chinese Han population. PMID: 29936663
  3. our data establish that blood TSAT exerts a critical role in experimental stroke-induced brain damage. In addition, our findings suggest that the protective effect of iron-free transferrin (apotransferrin, ATf) at the neuronal level resides in preventing NMDA-induced HTf uptake and ROS production, which in turn reduces neuronal damage PMID: 29248829
  4. Histochemical staining with anti-Tf antibody and an N-acetylglucosamine-binding lectin suggests that brain-type Tf is secreted from choroid plexus, CSF-producing tissue PMID: 29701803
  5. Data suggest that serum levels of biomarkers of iron status (ferritin, transferrin, and hemoglobin) are positively associated with risk of hyperuricemia in Chinese adults. PMID: 29425155
  6. Transfeerrin saturation was associated with less severe anemia in early CKD patients. PMID: 29227972
  7. Three single nucleotide polymorphisms associated with iron regulation were genotyped in multiple sclerosis : two in the human hereditary hemochromatosis protein gene HFE: rs1800562 (C282Y mutation) and rs1799945 (H63D mutation), as well as the rs1049296 SNP in the transferrin gene (C2 mutation). We only observed a higher prevalence of TF-C2 in multiple sclerosis patients PMID: 29201641
  8. Increased serum transferrin and ceruloplasmin turnover in diet-controlled patients with type 2 diabetes has been reported. PMID: 29079528
  9. model predicts that small vesicles promote activation of FX by the extrinsic tenase (VIIa/TF) significantly better than large vesicles PMID: 28935233
  10. This work presents an HPLC analysis of carbohydrate-deficient transferrin. PMID: 28971232
  11. Transferrin associated with Adenoid Cystic Carcinoma of Parotid Gland in immunohistochemical study, was found up-regulated in Pleomorphic Adenoma of the Parotid Gland. PMID: 28497265
  12. The RNAi knock-down of Trypanosoma brucei GSK3beta reduced Tf endocytosis. PMID: 27626104
  13. holotransferrin treatment to recombinant-TfR1 HepG2 cells did not elevate HAMP responses compared to untreated or wild-type cells. In PMID: 27667164
  14. Blood lead levels were positively associated with plasma homocysteine levels measured 4-6 years later, and three SNPs in the TF gene modified the association. PMID: 28775131
  15. In this technical note, the aberrant glycosylation profiles of transferrin glycoforms in congenital disorder of glycosylation patients are presented to shed light on the Mass Spectrometry of native transferrin and glycopeptides from the viewpoint of clinical glycoproteomics. PMID: 27095603
  16. Over the first 500 s, approximately 92,000 molecules of thrombin were generated per surface TF molecule for the 250-mum-long coating. PMID: 27605669
  17. High carbohydrate deficient transferrin is associated with relapse in alcohol-dependent patients. PMID: 26851349
  18. High Plasma Carbohydrate-deficient transferrin is associated with monoclonal light chain gammopathy. PMID: 27003373
  19. the value of transferrin receptors (TfRs)/cell reveals a 100-fold increase in the number of TfRs per bEnd3 cells compared to human umbilical vein cells. PMID: 27100851
  20. During pregnancy, the levels of 2-, 5- and 6-sialo transferrin isoforms were increasing while 3- and 4-sialo transferrin isoforms were decreasing. PMID: 27448041
  21. alterations in transferrin glycosylation/sialylation in pancreatic cancer differ from the alterations in some other cancers. PMID: 27312554
  22. In a multiple regression analysis, FN3K rs1056534, TF polymorphism and presence of diabetes mellitus were predictors for HHV-8 infection. PMID: 27461879
  23. the analysis of the hTf complex with a bacterial receptor that has evolved to sequester iron identifies two regions contacting rapidly evolving residues that mechanically manipulate dissociation from the pathogen PMID: 26955866
  24. Transferrin mutations can severely impair the diagnostics of chronic alcohol abuse. PMID: 26333807
  25. Total iron binding capacity was strongly associated with variants in and near the TF gene, and with variants in HFE gene in premenopausal women of European descent. PMID: 26852655
  26. Data suggest that up-regulation of serum transferrin levels is positively associated with risk of metabolic syndrome and overweight/obesity, but not with risk of diabetes among Chinese men and women. PMID: 25914390
  27. activation of PI3K/Akt by retinoic acid modulates AMPK activity in ECs and plays a crucial role in the inhibition of coagulatory factors such as TF, PAI-1, and HMGB1 in inflammatory conditions PMID: 26116962
  28. TF severed as the carrier to delivery irons, and could directly stimulate cardiomyocytes hypertrophy. PMID: 26099594
  29. Data show that transferrin (Tf) was attached to nanoparticles with an acid-cleavable linkage that facilities release of nanoparticles from Tf that are bound to Tf receptors (TfR) during transcytosis, promoting entry of the nanoparticles into the brain. PMID: 26392563
  30. H2O2 induces the expression of transferrin, and consequently, decreased iron absorption, suggests a novel mechanism for iron deficiency in pediatric non-alcoholic steatohepatitis patients. PMID: 25000850
  31. Human transferrin is internalized across the mycobacterial cell wall in a GAPDH-dependent manner within infected macrophages. PMID: 25163484
  32. Data suggest that the 15 disulfide bonds in transferrin vary in their susceptibility to reduction and in their contribution to protein conformation/stability. PMID: 25716754
  33. apelin-13 has a role in inducing expression of prothrombotic tissue factor PMID: 25298206
  34. identified the rs3811647 polymorphism in the TF gene as the only SNP significantly associated with iron metabolism through serum transferrin and iron levels PMID: 25457201
  35. significant associations between serum iron binding capacity levels and two SNPs around TF on chromosome 3 were identified. PMID: 25224454
  36. Hereditary hypotransferrinemia caused by novel transferrin mutations can lead to elevated transferrin saturation and, when associated with HFE or HAMP mutations, to iron overload. PMID: 25486930
  37. Results show that higher TF expression in patients with hypereosinophilic disorders may contribute to increase the thrombotic risk. PMID: 25375118
  38. genetic association studies in a population of black women in South Africa: Data confirm that an SNP in TF (rs1799852) is associated with iron status/iron-deficiency anemia in the population studied. PMID: 25809685
  39. decreased circulating levels in abdominal aortic aneurysm PMID: 24599423
  40. Infants born to mothers with the HFE C282Y gene missense variant but not the TF P570S or HFE H63D gene missense variants had lower umbilical cord blood lead levels relative to those born to women who were wild-type. PMID: 25287020
  41. TF translocates to the cell front in association with cytoskeleton proteins and regulates HVSMC migration by mechanisms dependent and independent of factor (F)VIIa/PAR2 PMID: 22938499
  42. did not find any association between the c.-2G>A polymorphism and keratoconus; no association was found between transferrin polymorphisms and Fuchs endothelial corneal dystrophy occurrence PMID: 24350254
  43. Understanding the interaction between [Cr(phen)3](3+) with transferrin is relevant because this protein could be a delivery agent of Cr(III) complex to tumor cells. PMID: 24972167
  44. Increased urinary excretion of plasma proteins such as IgG, ceruloplasmin and transferrin, with different molecular radii of 55 A or less and different isoelectric points precede development of microalbuminuria in patients with NIDDM and hypertension. PMID: 24256706
  45. Elevated BMI reduces the diagnostic utility of carbohydrate-deficient transferrin at higher alcohol intake in subjects with liver disease. PMID: 23875541
  46. Data suggest that there is no association between plasma level of carbohydrate-deficient transferrin and volume decrease of brain gray or white matter in male subjects as result of current/recent or lifetime alcohol drinking in the Netherlands. PMID: 23027680
  47. Studies indicate that several genes have been linked to iron homeostasis, including transferrin (TF), iron regulatory protein 1 (ACO1) and transferrin receptor 2 (TFR2). PMID: 23817740
  48. The A allele of SNP rs3811647 increases Tf expression in a manner that might underlie inter-individual variation in serum transferrin levels PMID: 23588470
  49. Transferrin mutations were found in several members of 2 non-consanguineous families with hypochromic microcytic anemia & hemodiserosis. The missense mutations were 1940 A>T & 1825 C>T. PMID: 23888904
  50. The structures of hTF observed here represent key conformers captured in the dynamic nature of the transferrin family proteins and provide a structural basis for understanding the mechanism of metal uptake and release in transferrin families. PMID: 23256035

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Involvement in disease
Atransferrinemia (ATRAF)
Subcellular Location
Secreted.
Protein Families
Transferrin family
Tissue Specificity
Expressed by the liver and secreted in plasma.
Database Links

HGNC: 11740

OMIM: 190000

KEGG: hsa:7018

STRING: 9606.ENSP00000385834

UniGene: Hs.518267

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