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Recombinant Human Serotransferrin(TF) (Active)

Recombinant Human Serotransferrin(TF) (Active)

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Code	CSB-MP023412HU
Abbreviation	Recombinant Human TF protein (Active)
MSDS	MSDS Datasheet
Size	$176
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel. Activity Measured by its binding ability in a functional ELISA. Immobilized TFRC(CSB-MP3648HU)at 2μg/mL can bind Human TF，the EC₅₀ is 58.72-77.84 ng/mL. Biological Activity Assay
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Target Background

Product Details

Purity

≥ 95% as determined by SDS-PAGE.

Endotoxin

Less than 1.0 EU/ug as determined by LAL method.

Activity

Measured by its binding ability in a functional ELISA. Immobilized Human TFRC(CSB-MP3648HU) at 2 μg/mL can bind Human TF. The EC50 is 58.72-77.84 ng/mL.

Target Names

Uniprot No.

P02787

Alternative Names

Transferrin;Beta-1 metal-binding globulin;Siderophilin;TF ;PRO1400

Species

Homo sapiens (Human)

Source

Mammalian cell

Expression Region

20-698aa

Target Protein Sequence

VPDKTVRWCAVSEHEATKCQSFRDHMKSVIPSDGPSVACVKKASYLDCIRAIAANEADAVTLDAGLVYDAYLAPNNLKPVVAEFYGSKEDPQTFYYAVAVVKKDSGFQMNQLRGKKSCHTGLGRSAGWNIPIGLLYCDLPEPRKPLEKAVANFFSGSCAPCADGTDFPQLCQLCPGCGCSTLNQYFGYSGAFKCLKDGAGDVAFVKHSTIFENLANKADRDQYELLCLDNTRKPVDEYKDCHLAQVPSHTVVARSMGGKEDLIWELLNQAQEHFGKDKSKEFQLFSSPHGKDLLFKDSAHGFLKVPPRMDAKMYLGYEYVTAIRNLREGTCPEAPTDECKPVKWCALSHHERLKCDEWSVNSVGKIECVSAETTEDCIAKIMNGEADAMSLDGGFVYIAGKCGLVPVLAENYNKSDNCEDTPEAGYFAIAVVKKSASDLTWDNLKGKKSCHTAVGRTAGWNIPMGLLYNKINHCRFDEFFSEGCAPGSKKDSSLCKLCMGSGLNLCEPNNKEGYYGYTGAFRCLVEKGDVAFVKHQTVPQNTGGKNPDPWAKNLNEKDYELLCLDGTRKPVEEYANCHLARAPNHAVVTRKDKEACVHKILRQQQHLFGSNVTDCSGNFCLFRSETKDLLFRDDTVCLAKLHDRNTYEKYLGEEYVKAVGNLRKCSTSSLLEACTFRRP

Mol. Weight

104.1kDa

Protein Length

Full Length of Mature Protein

Tag Info

C-terminal hFc1-tagged

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

Serotransferrin mediates iron delivery to cells via transferrin receptor (TFRC) binding, a pathway frequently dysregulated in cancer where elevated TFRC expression supports rapid proliferation. This full-length mature protein (aa 20–698) demonstrates quantified receptor engagement, binding immobilized human TFRC with an EC50 of 58.72–77.84 ng/mL in functional ELISA, confirming the conformational integrity required for ligand-binding assays and membrane transporter functional studies. The C-terminal hFc1 tag facilitates purification and detection without compromising the iron-binding domains, enabling this protein to serve as a reagent in drug-protein binding studies that investigate therapeutic conjugates or small molecules targeting the transferrin system. With purity exceeding 95% by SDS-PAGE and endotoxin levels below 1.0 EU/μg, this preparation meets the quality thresholds commonly required for antibody development, ELISA standardization, and biophysical characterization experiments examining transferrin's role in iron homeostasis and cancer metabolism.

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Target Background

Function

Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation.; (Microbial infection) Serves as an iron source for Neisseria species, which capture the protein and extract its iron for their own use.

Gene References into Functions

High transferrin expression is associated with Spontaneous intracranial hypotension. PMID: 29621631
rs4525863, rs1880669, and rs2692695 SNPs of the TF gene might be a protective factor for ischemic stroke in Southern Chinese Han population. PMID: 29936663
our data establish that blood TSAT exerts a critical role in experimental stroke-induced brain damage. In addition, our findings suggest that the protective effect of iron-free transferrin (apotransferrin, ATf) at the neuronal level resides in preventing NMDA-induced HTf uptake and ROS production, which in turn reduces neuronal damage PMID: 29248829
Histochemical staining with anti-Tf antibody and an N-acetylglucosamine-binding lectin suggests that brain-type Tf is secreted from choroid plexus, CSF-producing tissue PMID: 29701803
Data suggest that serum levels of biomarkers of iron status (ferritin, transferrin, and hemoglobin) are positively associated with risk of hyperuricemia in Chinese adults. PMID: 29425155
Transfeerrin saturation was associated with less severe anemia in early CKD patients. PMID: 29227972
Three single nucleotide polymorphisms associated with iron regulation were genotyped in multiple sclerosis : two in the human hereditary hemochromatosis protein gene HFE: rs1800562 (C282Y mutation) and rs1799945 (H63D mutation), as well as the rs1049296 SNP in the transferrin gene (C2 mutation). We only observed a higher prevalence of TF-C2 in multiple sclerosis patients PMID: 29201641
Increased serum transferrin and ceruloplasmin turnover in diet-controlled patients with type 2 diabetes has been reported. PMID: 29079528
model predicts that small vesicles promote activation of FX by the extrinsic tenase (VIIa/TF) significantly better than large vesicles PMID: 28935233
This work presents an HPLC analysis of carbohydrate-deficient transferrin. PMID: 28971232
Transferrin associated with Adenoid Cystic Carcinoma of Parotid Gland in immunohistochemical study, was found up-regulated in Pleomorphic Adenoma of the Parotid Gland. PMID: 28497265
The RNAi knock-down of Trypanosoma brucei GSK3beta reduced Tf endocytosis. PMID: 27626104
holotransferrin treatment to recombinant-TfR1 HepG2 cells did not elevate HAMP responses compared to untreated or wild-type cells. In PMID: 27667164
Blood lead levels were positively associated with plasma homocysteine levels measured 4-6 years later, and three SNPs in the TF gene modified the association. PMID: 28775131
In this technical note, the aberrant glycosylation profiles of transferrin glycoforms in congenital disorder of glycosylation patients are presented to shed light on the Mass Spectrometry of native transferrin and glycopeptides from the viewpoint of clinical glycoproteomics. PMID: 27095603
Over the first 500 s, approximately 92,000 molecules of thrombin were generated per surface TF molecule for the 250-mum-long coating. PMID: 27605669
High carbohydrate deficient transferrin is associated with relapse in alcohol-dependent patients. PMID: 26851349
High Plasma Carbohydrate-deficient transferrin is associated with monoclonal light chain gammopathy. PMID: 27003373
the value of transferrin receptors (TfRs)/cell reveals a 100-fold increase in the number of TfRs per bEnd3 cells compared to human umbilical vein cells. PMID: 27100851
During pregnancy, the levels of 2-, 5- and 6-sialo transferrin isoforms were increasing while 3- and 4-sialo transferrin isoforms were decreasing. PMID: 27448041
alterations in transferrin glycosylation/sialylation in pancreatic cancer differ from the alterations in some other cancers. PMID: 27312554
In a multiple regression analysis, FN3K rs1056534, TF polymorphism and presence of diabetes mellitus were predictors for HHV-8 infection. PMID: 27461879
the analysis of the hTf complex with a bacterial receptor that has evolved to sequester iron identifies two regions contacting rapidly evolving residues that mechanically manipulate dissociation from the pathogen PMID: 26955866
Transferrin mutations can severely impair the diagnostics of chronic alcohol abuse. PMID: 26333807
Total iron binding capacity was strongly associated with variants in and near the TF gene, and with variants in HFE gene in premenopausal women of European descent. PMID: 26852655
Data suggest that up-regulation of serum transferrin levels is positively associated with risk of metabolic syndrome and overweight/obesity, but not with risk of diabetes among Chinese men and women. PMID: 25914390
activation of PI3K/Akt by retinoic acid modulates AMPK activity in ECs and plays a crucial role in the inhibition of coagulatory factors such as TF, PAI-1, and HMGB1 in inflammatory conditions PMID: 26116962
TF severed as the carrier to delivery irons, and could directly stimulate cardiomyocytes hypertrophy. PMID: 26099594
Data show that transferrin (Tf) was attached to nanoparticles with an acid-cleavable linkage that facilities release of nanoparticles from Tf that are bound to Tf receptors (TfR) during transcytosis, promoting entry of the nanoparticles into the brain. PMID: 26392563
H2O2 induces the expression of transferrin, and consequently, decreased iron absorption, suggests a novel mechanism for iron deficiency in pediatric non-alcoholic steatohepatitis patients. PMID: 25000850
Human transferrin is internalized across the mycobacterial cell wall in a GAPDH-dependent manner within infected macrophages. PMID: 25163484
Data suggest that the 15 disulfide bonds in transferrin vary in their susceptibility to reduction and in their contribution to protein conformation/stability. PMID: 25716754
apelin-13 has a role in inducing expression of prothrombotic tissue factor PMID: 25298206
identified the rs3811647 polymorphism in the TF gene as the only SNP significantly associated with iron metabolism through serum transferrin and iron levels PMID: 25457201
significant associations between serum iron binding capacity levels and two SNPs around TF on chromosome 3 were identified. PMID: 25224454
Hereditary hypotransferrinemia caused by novel transferrin mutations can lead to elevated transferrin saturation and, when associated with HFE or HAMP mutations, to iron overload. PMID: 25486930
Results show that higher TF expression in patients with hypereosinophilic disorders may contribute to increase the thrombotic risk. PMID: 25375118
genetic association studies in a population of black women in South Africa: Data confirm that an SNP in TF (rs1799852) is associated with iron status/iron-deficiency anemia in the population studied. PMID: 25809685
decreased circulating levels in abdominal aortic aneurysm PMID: 24599423
Infants born to mothers with the HFE C282Y gene missense variant but not the TF P570S or HFE H63D gene missense variants had lower umbilical cord blood lead levels relative to those born to women who were wild-type. PMID: 25287020
TF translocates to the cell front in association with cytoskeleton proteins and regulates HVSMC migration by mechanisms dependent and independent of factor (F)VIIa/PAR2 PMID: 22938499
did not find any association between the c.-2G>A polymorphism and keratoconus; no association was found between transferrin polymorphisms and Fuchs endothelial corneal dystrophy occurrence PMID: 24350254
Understanding the interaction between [Cr(phen)3](3+) with transferrin is relevant because this protein could be a delivery agent of Cr(III) complex to tumor cells. PMID: 24972167
Increased urinary excretion of plasma proteins such as IgG, ceruloplasmin and transferrin, with different molecular radii of 55 A or less and different isoelectric points precede development of microalbuminuria in patients with NIDDM and hypertension. PMID: 24256706
Elevated BMI reduces the diagnostic utility of carbohydrate-deficient transferrin at higher alcohol intake in subjects with liver disease. PMID: 23875541
Data suggest that there is no association between plasma level of carbohydrate-deficient transferrin and volume decrease of brain gray or white matter in male subjects as result of current/recent or lifetime alcohol drinking in the Netherlands. PMID: 23027680
Studies indicate that several genes have been linked to iron homeostasis, including transferrin (TF), iron regulatory protein 1 (ACO1) and transferrin receptor 2 (TFR2). PMID: 23817740
The A allele of SNP rs3811647 increases Tf expression in a manner that might underlie inter-individual variation in serum transferrin levels PMID: 23588470
Transferrin mutations were found in several members of 2 non-consanguineous families with hypochromic microcytic anemia & hemodiserosis. The missense mutations were 1940 A>T & 1825 C>T. PMID: 23888904
The structures of hTF observed here represent key conformers captured in the dynamic nature of the transferrin family proteins and provide a structural basis for understanding the mechanism of metal uptake and release in transferrin families. PMID: 23256035