Monkey Interferon γ ,IFN-γ ELISA Kit

This ELISA kit provides quantitative measurement of interferon-gamma (IFN-γ) in monkey samples, supporting immunological research where this cytokine serves as a central mediator of Th1-driven immune responses. Because IFN-γ is often measured alongside other cytokines to assess immune activation status in nonhuman primate models of infection, vaccination, or transplantation, this kit is designed to address the specific demands of those study designs.

Detection Range: The 6.25–400 pg/mL range captures IFN-γ concentrations typical of antigen-stimulated PBMC supernatants and serum from immune-challenged primates, where levels frequently fall between 10 and 300 pg/mL. Unstimulated baseline samples generally require no dilution, while mitogen-stimulated cultures may need predilution.

Sensitivity: At 3.125 pg/mL, the kit detects the low-level IFN-γ secretion characteristic of resting or minimally stimulated immune cells, enabling measurement in unstimulated control conditions and early post-vaccination timepoints.

Sample Compatibility: Validation across serum, plasma, cell culture supernatants, and tissue homogenates allows researchers to correlate systemic IFN-γ levels with localized tissue responses and ex vivo cellular recall assays within a single study.

Assay Efficiency: The 1–5 hour protocol and 50–100 µL sample volume accommodate limited-volume primate specimens and high-throughput screening of multiple stimulation conditions from small PBMC cultures.

This kit is particularly suited for NHP vaccine immunogenicity studies, tuberculosis infection models, and graft rejection monitoring.