Monkey monocyte chemotactic protein 1/monocyte chemotactic and activating factor,MCP1/MCAF ELISA kit

Code CSB-E10045Mo
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Description

This Monkey CCL2 ELISA Kit was designed for the quantitative measurement of Monkey CCL2 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 62.5 pg/mL-4000 pg/mL and the sensitivity is 15.6 pg/mL .

Target Name chemokine (C-C motif) ligand 2
Alternative Names CCL2 ELISA kit; MCP1 ELISA kit; SCYA2C-C motif chemokine 2 ELISA kit; Monocyte chemoattractant protein 1 ELISA kit; Monocyte chemotactic protein 1 ELISA kit; MCP-1 ELISA kit; Small-inducible cytokine A2 ELISA kit
Abbreviation CCL2
Uniprot No. P61275
Species Monkey
Sample Types serum, plasma, tissue homogenates
Detection Range 62.5 pg/mL-4000 pg/mL
Sensitivity 15.6 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
4000 2.418 2.352 2.385 2.186  
2000 1.982 1.876 1.929 1.730  
1000 1.413 1.404 1.409 1.210  
500 0.816 0.961 0.889 0.690  
250 0.543 0.524 0.534 0.335  
125 0.374 0.359 0.367 0.168  
62.5 0.302 0.289 0.296 0.097  
0 0.201 0.197 0.199    
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
Acts as a ligand for C-C chemokine receptor CCR2. Signals through binding and activation of CCR2 and induces a strong chemotactic response and mobilization of intracellular calcium ions. Exhibits a chemotactic activity for monocytes and basophils but not neutrophils or eosinophils. Plays an important role in mediating peripheral nerve injury-induced neuropathic pain. Increases NMDA-mediated synaptic transmission in both dopamine D1 and D2 receptor-containing neurons, which may be caused by MAPK/ERK-dependent phosphorylation of GRIN2B/NMDAR2B.
Subcellular Location Secreted.
Protein Families Intercrine beta (chemokine CC) family
Database Links

KEGG: mcc:574138

STRING: 9544.ENSMMUP00000000391

UniGene: Mmu.3491

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