Mouse Bone morphogenetic protein 4,BMP-4 ELISA Kit

Instructions
Code CSB-E04512m
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application

Product Details

Target Name bone morphogenetic protein 4
Alternative Names BMP2B, BMP2B1, MCOPS6, OFC11, ZYME, bone morphogenetic protein 2B
Abbreviation BMP4
Uniprot No. P21275
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.625 pg/mL-40 pg/mL
Sensitivity 0.156 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse BMP-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 85  
Range % 80-95  
1:2 Average % 96  
Range % 91-112  
1:4 Average % 85  
Range % 80-110  
1:8 Average % 90  
Range % 86-98  
Recovery
The recovery of mouse BMP-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 94 89-98  
EDTA plasma (n=4) 92 90-99  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
40 2.657 2.677 2.667 2.509  
20 2.212 2.302 2.257 2.099  
10 1.583 1.683 1.633 1.475  
5 1.042 1.082 1.062 0.904  
2.5 0.597 0.622 0.610 0.452  
1.25 0.384 0.401 0.393 0.235  
0.625 0.288 0.302 0.295 0.137  
0 0.162 0.154 0.158    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days
Still Have Questions? Leave a Message or Start an on-line Chat

Target Data

Function Induces cartilage and bone formation. Acts in concert with PTHLH/PTHRP to stimulate ductal outgrowth during embryonic mammary development and to inhibit hair follicle induction.
Subcellular Location Secreted, extracellular space, extracellular matrix
Protein Families TGF-beta family
Database Links

KEGG: mmu:12159

STRING: 10090.ENSMUSP00000073720

UniGene: Mm.6813

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