Mouse Bone morphogenetic protein 4,BMP-4 ELISA Kit

Instructions
Code CSB-E04512m
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name bone morphogenetic protein 4
Alternative Names Bmp4 ELISA kit; Bmp-4 ELISA kit; Dvr-4Bone morphogenetic protein 4 ELISA kit; BMP-4 ELISA kit; Bone morphogenetic protein 2B ELISA kit; BMP-2B ELISA kit
Abbreviation BMP4
Uniprot No. P21275
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.625 pg/mL-40 pg/mL
Sensitivity 0.156 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse BMP-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 85  
Range % 80-95  
1:2 Average % 96  
Range % 91-112  
1:4 Average % 85  
Range % 80-110  
1:8 Average % 90  
Range % 86-98  
Recovery
The recovery of mouse BMP-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 94 89-98  
EDTA plasma (n=4) 92 90-99  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
40 2.657 2.677 2.667 2.509  
20 2.212 2.302 2.257 2.099  
10 1.583 1.683 1.633 1.475  
5 1.042 1.082 1.062 0.904  
2.5 0.597 0.622 0.610 0.452  
1.25 0.384 0.401 0.393 0.235  
0.625 0.288 0.302 0.295 0.137  
0 0.162 0.154 0.158    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
Induces cartilage and bone formation. Acts in concert with PTHLH/PTHRP to stimulate ductal outgrowth during embryonic mammary development and to inhibit hair follicle induction.
Gene References into Functions
  1. The importance of the BMP4 pathway in endogenous tissue regeneration and offer a potential clinical approach to enhance T cell immunity. PMID: 29330161
  2. BMP4 and IL7 appear to be involved in the interaction between intestinal epithelial cells and intestinal epithelial cells and in the mechanism underlying intestinal mucosal barrier dysfunction. PMID: 29436597
  3. Knockdown of BMP4 in pregnant mice at the middle embryonic stage led to aganglionosis. PMID: 29393463
  4. we demonstrated that the anterior and posterior phenotypes observed in Bmp4 heterozygous animals showed a strong propensity to co-occur, suggesting a common, non-cell autonomous source for these defects. PMID: 29738572
  5. MiR-876-5p suppresses epithelial-mesenchymal transition of lung cancer by directly down-regulating BMP-4. PMID: 29229885
  6. p73 is required for appropriate BMP-induced mesenchymal-to-epithelial transition during somatic cell reprogramming. PMID: 28880267
  7. BMP4 inhibits type 2 epithelial cells (AT2s) proliferation, whereas antagonists (follistatin, noggin) promote AT2 self-renewal at the expense of differentiation. Gain- and loss-of-function genetic manipulation reveals that reduced BMP signaling in AT2s after PNX allows self-renewal but reduces differentiation PMID: 29752282
  8. this study shows that negative autoregulation of BMP4 dependent transcription by SIN3B splicing reveals a role for RBM39 PMID: 27324164
  9. Gene expression profiling showed that MuSK was required for the BMP4-induced expression of a subset of genes in myoblasts, including regulator of G protein signaling 4 (Rgs4). PMID: 27601729
  10. differentiated cells exhibited contracting masses. These results suggest that BMP4-mediated somatic stem cell reprogramming may become an alternative approach for cell therapy PMID: 28170287
  11. Bmp4 promotes a brown to white-like adipocyte shift. PMID: 27524617
  12. results suggest that bone morphogenetic protein 4 promotes the generation of male germ cells from induced pluripotent stem (iPS) cells PMID: 27864336
  13. These results suggest how BMP4 regulates adipocyte recruitment in subcutaneous (SC) white adipose tissue, and thus promote its beneficial metabolic effects. PMID: 27030507
  14. RUNX1T1 serves as a common angiogenic driver for vaculogenesis and functionality of endothelial lineage cells PMID: 28640846
  15. Expression of Bmp4 in the ureteric mesenchyme depends on HH signaling and Foxf1, and that exogenous BMP4 rescued cell proliferation and epithelial differentiation in ureters with abrogated HH signaling or FOXF1 function. PMID: 28797033
  16. These data indicate that Foxc1 expression is regulated by BMP4 and FOXC1 functions in the commitment of progenitor cells to the osteoblast fate and its expression is reduced when differentiation proceeds. PMID: 26666591
  17. Phosphorylated Smad1/5/8/9 specifically bound to the BREs of Smad8/9 gene. The present study reveals that Smad8/9 is a unique R-Smad regulated through the BMP pathway at the mRNA level. PMID: 26748560
  18. Treatment of ES cells with TPO induced the autocrine production of BMP4 with concomitant upregulation of the BMP receptor BMPR1A, phosphorylation of SMAD1, 5, 8, and activation of specific BMP4 target genes; this was mediated by TPO-dependent binding of transcription factor HIF-1alpha to the BMP4 gene promoter. PMID: 27447537
  19. Data suggest that ovarian Bmp4 levels are significantly decreased in a mouse model of polycystic ovary syndrome with hyperandrogenism; androgens inhibited Bmp4 expression via activation of androgen receptors; Smad4 signaling rather than p38 MAPK pathway regulates androgen and estrogen formation. PMID: 28572510
  20. tightly spatial interaction of FSTL1 and BMP4 signaling plays an essential role in lung development PMID: 27355685
  21. this study identified Bmp4 as a significant factor for improved maintenance of Foxn1 expression, promotion of thymic epithelial progenitor populations self-renewal and immature epithelial cell differentiation and survival PMID: 27861793
  22. these data reveal a novel mechanism that the Bmp4-Msx1 pathway and Osr2 control tooth organogenesis through antagonistic regulation of expression of secreted Wnt antagonists. PMID: 27713059
  23. inhibitory role on glucagon secretion, pancreatic alpha-cell growth, and expression of genes maintaining alpha-cell identity PMID: 27479530
  24. We demonstrate that elevated BMP4 depletes PSM progenitors in vitro, phenocopying the Fgf3 mutant, suggesting that excessive BMP signals cause the Fgf3 axis defect. To test this in vivo we increased BMP signaling in Fgf3 mutants by removing one copy of Noggin, which encodes a BMP antagonist. PMID: 27144312
  25. BMP-4 inhibited insulin signaling through activation of PKC-theta; isoform, and resulted in insulin resistance through the attenuation of insulin signaling PMID: 28186649
  26. BMP4 inhibited ERalpha signaling by increasing the degradation of ERalpha, and that the expression of BMP4 itself was suppressed by estrogens. PMID: 27522322
  27. the present study suggested that miRNA378 is critical for the promotion of myoblast differentiation by targeting BMP4. PMID: 26782975
  28. Increased BMP signaling contributes significantly to impairments in neurogenesis and to cognitive decline associated with aging PMID: 26827654
  29. findings suggest that Sox6 contributes to cell survival by suppressing BMP-4 transcription during neuronal differentiation PMID: 26590087
  30. Data show that R-Smad Proteins SMAD1 and SMAD5, which transduce bone morphogenetic protein (BMP) signals, recognize enhancer regions together with Kruppel-like factors KLF4 and KLF5 in naive embryonic stem cell (mESCs). PMID: 26771354
  31. Ino80 represses Bmp4 expression during embryonic stem cell differentiation. PMID: 26975355
  32. PDGF-AA impairs endothelium-dependent vasodilation and PDGF-AA mediates BMP4-induced adverse effect on endothelial cell function through SMAD1/5- and SMAD4-dependent mechanisms. PMID: 26769046
  33. BMP4 has a role in inducing NOX1-dependent eNOS uncoupling in T2DM, which may promote development of novel therapeutics restoring endothelial function in T2DM PMID: 26121233
  34. resveratrol markedly amplifies BMP4induced OPG mRNA expression. PMID: 26044505
  35. BMP4 promotes activation of FGF7 and FGF10 signaling, leading to an increase in proliferative basal cell population in developing skin. PMID: 25329657
  36. calbindin1 in rat primary islet cells reduced glucose-stimulated insulin secretion (GSIS), and the effect of BMP4 on GSIS was lost in islets from calbindin1 (Calb1) knockout mice PMID: 25828920
  37. Wnt/beta-catenin signaling modulates corneal epithelium stratification via inhibition of Bmp4 during mouse development. PMID: 26443636
  38. Bmp4, Bmp7 and bmpr2 signalling regulates the pre-implantation development of extra-embryonic cell lineages in the mouse embryo PMID: 25514175
  39. Transfection of Pax9 and Bmp4 into mouse induced pluripotent stem cells facilitates their differentiation into odontoblast-like cells for cell- and tissue-based therapy. PMID: 26136503
  40. the BMP4 prodomain is necessary and sufficient to generate stable BMP4/7 heterodimers PMID: 25902523
  41. Bmp4 from the optic vesicle has a role in murine retina formation PMID: 25792196
  42. BMP4-induced upregulation of Cav3.1 mRNA. PMID: 24510064
  43. Megf8 is involved in mediating BMP4 signaling and guidance of developing trigeminal ganglia axons. PMID: 24052814
  44. A transient wave of BMP signaling in the retina is necessary for Muller glial differentiation. PMID: 25605781
  45. BMP4 plays an important anti-inflammatory role, controlling the strength and facilitating the resolution of acute lung inflammation; yet, BMP4 also contributes to lung function impairment during chronic lung inflammation. PMID: 25142202
  46. BMP4 reduces the expression and secretion of G-CSF by inhibiting NF-kappaB (Nfkb1) activity in human and mouse tumor lines. PMID: 25224959
  47. BMP4 induced lipid-laden foam cell formation by inhibiting cholesterol efflux via ABCA1 and ABCG1 through the BMPR-2/Smad1/2/8 signaling pathway. PMID: 24690996
  48. Bone morphogenetic protein-1/mTLD and PCPE-1 are upregulated in corneal scars. Both proteins may therefore contribute to the process of corneal scarring. PMID: 25249603
  49. the transient BMP4 prodomain/ligand complex that forms during sequential cleavage plays an essential role in prodomain-mediated stabilization of the mature ligand PMID: 24993941
  50. BMP4 signaling reduces inflammation and inhibits dysplastic changes in the gastric mucosa after infection of mice with H pylori or H felis. PMID: 24751878

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Subcellular Location Secreted, extracellular space, extracellular matrix
Protein Families TGF-beta family
Database Links

KEGG: mmu:12159

STRING: 10090.ENSMUSP00000073720

UniGene: Mm.6813

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