Mouse Bone morphogenetic protein 4,BMP-4 ELISA Kit

Code CSB-E04512m
See More Details
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P21275
Lead Time 3-5 working days
Abbreviation BMP4
Protein Biological Process 1 Developmental Protein
Target Name bone morphogenetic protein 4
Alias BMP2B, BMP2B1, MCOPS6, OFC11, ZYME, bone morphogenetic protein 2B
Species Mus musculus (Mouse)
Protein Biological Process 3 Chondrogenesis
Sample Types serum, plasma, tissue homogenates
Detection Range 0.625 pg/mL-40 pg/mL
Sensitivity 0.156 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This protein is a member of the bone morphogenetic protein family which is part of the transforming growth factor-beta superfamily. The superfamily includes large families of growth and differentiation factors. Bone morphogenetic proteins were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site. This particular family member plays an important role in the onset of endochondral bone formation in humans, and a reduction in expression has been associated with a variety of bone diseases, including the heritable disorder Fibrodysplasia Ossificans Progressiva. Alternative splicing in the 5 untranslated region of this gene has been described and three variants are described, all encoding an identical protein.
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse BMP-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 85  
Range % 80-95  
1:2 Average % 96  
Range % 91-112  
1:4 Average % 85  
Range % 80-110  
1:8 Average % 90  
Range % 86-98  
Recovery
The recovery of mouse BMP-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 94 89-98  
EDTA plasma (n=4) 92 90-99  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
40 2.657 2.677 2.667 2.509  
20 2.212 2.302 2.257 2.099  
10 1.583 1.683 1.633 1.475  
5 1.042 1.082 1.062 0.904  
2.5 0.597 0.622 0.610 0.452  
1.25 0.384 0.401 0.393 0.235  
0.625 0.288 0.302 0.295 0.137  
0 0.162 0.154 0.158    
References
Still Have Questions? Leave a Message or Start an on-line Chat
Function Induces cartilage and bone formation. Acts in concert with PTHLH/PTHRP to stimulate ductal outgrowth during embryonic mammary development and to inhibit hair follicle induction.
Subcellular Location Secreted, extracellular space, extracellular matrix
Protein Families TGF-beta family
Database Links

KEGG: mmu:12159

STRING: 10090.ENSMUSP00000073720

UniGene: Mm.6813

Pathway Hippo signaling pathway
TGF-beta signaling pathway
Signaling pathways regulating pluripotency of stem cells

Related Products

Bmp4 Proteins

Bmp4 Proteins for Mus musculus (Mouse)

Most popular with customers

Newsletters

Get all the latest information on Events, Sales and Offers. Sign up for newsletter today.

Copyright © 2007-2018 www.cusabio.com CUSABIO TECHNOLOGY LLC All Rights Reserved.

Wait!

Join the 25,000 subscribers to get research hotpots, technical tips, latest information on events, sales and offers.

Sign up now!

We don't deal in spam.