Mouse Bone morphogenetic protein 4,BMP-4 ELISA Kit

Instructions
Code CSB-E04512m
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name bone morphogenetic protein 4
Alternative Names Bmp4 ELISA kit; Bmp-4 ELISA kit; Dvr-4Bone morphogenetic protein 4 ELISA kit; BMP-4 ELISA kit; Bone morphogenetic protein 2B ELISA kit; BMP-2B ELISA kit
Abbreviation BMP4
Uniprot No. P21275
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.625 pg/mL-40 pg/mL
Sensitivity 0.156 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse BMP-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 85  
Range % 80-95  
1:2 Average % 96  
Range % 91-112  
1:4 Average % 85  
Range % 80-110  
1:8 Average % 90  
Range % 86-98  
Recovery
The recovery of mouse BMP-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 94 89-98  
EDTA plasma (n=4) 92 90-99  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
40 2.657 2.677 2.667 2.509  
20 2.212 2.302 2.257 2.099  
10 1.583 1.683 1.633 1.475  
5 1.042 1.082 1.062 0.904  
2.5 0.597 0.622 0.610 0.452  
1.25 0.384 0.401 0.393 0.235  
0.625 0.288 0.302 0.295 0.137  
0 0.162 0.154 0.158    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Target Data

Function Induces cartilage and bone formation. Acts in concert with PTHLH/PTHRP to stimulate ductal outgrowth during embryonic mammary development and to inhibit hair follicle induction.
Gene References into Functions
  1. The importance of the BMP4 pathway in endogenous tissue regeneration and offer a potential clinical approach to enhance T cell immunity. PMID: 29330161
  2. BMP4 and IL7 appear to be involved in the interaction between intestinal epithelial cells and intestinal epithelial cells and in the mechanism underlying intestinal mucosal barrier dysfunction. PMID: 29436597
  3. Knockdown of BMP4 in pregnant mice at the middle embryonic stage led to aganglionosis. PMID: 29393463
  4. we demonstrated that the anterior and posterior phenotypes observed in Bmp4 heterozygous animals showed a strong propensity to co-occur, suggesting a common, non-cell autonomous source for these defects. PMID: 29738572
  5. MiR-876-5p suppresses epithelial-mesenchymal transition of lung cancer by directly down-regulating BMP-4. PMID: 29229885
  6. p73 is required for appropriate BMP-induced mesenchymal-to-epithelial transition during somatic cell reprogramming. PMID: 28880267
  7. BMP4 inhibits type 2 epithelial cells (AT2s) proliferation, whereas antagonists (follistatin, noggin) promote AT2 self-renewal at the expense of differentiation. Gain- and loss-of-function genetic manipulation reveals that reduced BMP signaling in AT2s after PNX allows self-renewal but reduces differentiation PMID: 29752282
  8. this study shows that negative autoregulation of BMP4 dependent transcription by SIN3B splicing reveals a role for RBM39 PMID: 27324164
  9. Gene expression profiling showed that MuSK was required for the BMP4-induced expression of a subset of genes in myoblasts, including regulator of G protein signaling 4 (Rgs4). PMID: 27601729
  10. differentiated cells exhibited contracting masses. These results suggest that BMP4-mediated somatic stem cell reprogramming may become an alternative approach for cell therapy PMID: 28170287
  11. Bmp4 promotes a brown to white-like adipocyte shift. PMID: 27524617
  12. results suggest that bone morphogenetic protein 4 promotes the generation of male germ cells from induced pluripotent stem (iPS) cells PMID: 27864336
  13. These results suggest how BMP4 regulates adipocyte recruitment in subcutaneous (SC) white adipose tissue, and thus promote its beneficial metabolic effects. PMID: 27030507
  14. RUNX1T1 serves as a common angiogenic driver for vaculogenesis and functionality of endothelial lineage cells PMID: 28640846
  15. Expression of Bmp4 in the ureteric mesenchyme depends on HH signaling and Foxf1, and that exogenous BMP4 rescued cell proliferation and epithelial differentiation in ureters with abrogated HH signaling or FOXF1 function. PMID: 28797033
  16. These data indicate that Foxc1 expression is regulated by BMP4 and FOXC1 functions in the commitment of progenitor cells to the osteoblast fate and its expression is reduced when differentiation proceeds. PMID: 26666591
  17. Phosphorylated Smad1/5/8/9 specifically bound to the BREs of Smad8/9 gene. The present study reveals that Smad8/9 is a unique R-Smad regulated through the BMP pathway at the mRNA level. PMID: 26748560
  18. Treatment of ES cells with TPO induced the autocrine production of BMP4 with concomitant upregulation of the BMP receptor BMPR1A, phosphorylation of SMAD1, 5, 8, and activation of specific BMP4 target genes; this was mediated by TPO-dependent binding of transcription factor HIF-1alpha to the BMP4 gene promoter. PMID: 27447537
  19. Data suggest that ovarian Bmp4 levels are significantly decreased in a mouse model of polycystic ovary syndrome with hyperandrogenism; androgens inhibited Bmp4 expression via activation of androgen receptors; Smad4 signaling rather than p38 MAPK pathway regulates androgen and estrogen formation. PMID: 28572510
  20. tightly spatial interaction of FSTL1 and BMP4 signaling plays an essential role in lung development PMID: 27355685
  21. this study identified Bmp4 as a significant factor for improved maintenance of Foxn1 expression, promotion of thymic epithelial progenitor populations self-renewal and immature epithelial cell differentiation and survival PMID: 27861793
  22. these data reveal a novel mechanism that the Bmp4-Msx1 pathway and Osr2 control tooth organogenesis through antagonistic regulation of expression of secreted Wnt antagonists. PMID: 27713059
  23. inhibitory role on glucagon secretion, pancreatic alpha-cell growth, and expression of genes maintaining alpha-cell identity PMID: 27479530
  24. We demonstrate that elevated BMP4 depletes PSM progenitors in vitro, phenocopying the Fgf3 mutant, suggesting that excessive BMP signals cause the Fgf3 axis defect. To test this in vivo we increased BMP signaling in Fgf3 mutants by removing one copy of Noggin, which encodes a BMP antagonist. PMID: 27144312
  25. BMP-4 inhibited insulin signaling through activation of PKC-theta; isoform, and resulted in insulin resistance through the attenuation of insulin signaling PMID: 28186649
  26. BMP4 inhibited ERalpha signaling by increasing the degradation of ERalpha, and that the expression of BMP4 itself was suppressed by estrogens. PMID: 27522322
  27. the present study suggested that miRNA378 is critical for the promotion of myoblast differentiation by targeting BMP4. PMID: 26782975
  28. Increased BMP signaling contributes significantly to impairments in neurogenesis and to cognitive decline associated with aging PMID: 26827654
  29. findings suggest that Sox6 contributes to cell survival by suppressing BMP-4 transcription during neuronal differentiation PMID: 26590087
  30. Data show that R-Smad Proteins SMAD1 and SMAD5, which transduce bone morphogenetic protein (BMP) signals, recognize enhancer regions together with Kruppel-like factors KLF4 and KLF5 in naive embryonic stem cell (mESCs). PMID: 26771354
  31. Ino80 represses Bmp4 expression during embryonic stem cell differentiation. PMID: 26975355
  32. PDGF-AA impairs endothelium-dependent vasodilation and PDGF-AA mediates BMP4-induced adverse effect on endothelial cell function through SMAD1/5- and SMAD4-dependent mechanisms. PMID: 26769046
  33. BMP4 has a role in inducing NOX1-dependent eNOS uncoupling in T2DM, which may promote development of novel therapeutics restoring endothelial function in T2DM PMID: 26121233
  34. resveratrol markedly amplifies BMP4induced OPG mRNA expression. PMID: 26044505
  35. BMP4 promotes activation of FGF7 and FGF10 signaling, leading to an increase in proliferative basal cell population in developing skin. PMID: 25329657
  36. calbindin1 in rat primary islet cells reduced glucose-stimulated insulin secretion (GSIS), and the effect of BMP4 on GSIS was lost in islets from calbindin1 (Calb1) knockout mice PMID: 25828920
  37. Wnt/beta-catenin signaling modulates corneal epithelium stratification via inhibition of Bmp4 during mouse development. PMID: 26443636
  38. Bmp4, Bmp7 and bmpr2 signalling regulates the pre-implantation development of extra-embryonic cell lineages in the mouse embryo PMID: 25514175
  39. Transfection of Pax9 and Bmp4 into mouse induced pluripotent stem cells facilitates their differentiation into odontoblast-like cells for cell- and tissue-based therapy. PMID: 26136503
  40. the BMP4 prodomain is necessary and sufficient to generate stable BMP4/7 heterodimers PMID: 25902523
  41. Bmp4 from the optic vesicle has a role in murine retina formation PMID: 25792196
  42. BMP4-induced upregulation of Cav3.1 mRNA. PMID: 24510064
  43. Megf8 is involved in mediating BMP4 signaling and guidance of developing trigeminal ganglia axons. PMID: 24052814
  44. A transient wave of BMP signaling in the retina is necessary for Muller glial differentiation. PMID: 25605781
  45. BMP4 plays an important anti-inflammatory role, controlling the strength and facilitating the resolution of acute lung inflammation; yet, BMP4 also contributes to lung function impairment during chronic lung inflammation. PMID: 25142202
  46. BMP4 reduces the expression and secretion of G-CSF by inhibiting NF-kappaB (Nfkb1) activity in human and mouse tumor lines. PMID: 25224959
  47. BMP4 induced lipid-laden foam cell formation by inhibiting cholesterol efflux via ABCA1 and ABCG1 through the BMPR-2/Smad1/2/8 signaling pathway. PMID: 24690996
  48. Bone morphogenetic protein-1/mTLD and PCPE-1 are upregulated in corneal scars. Both proteins may therefore contribute to the process of corneal scarring. PMID: 25249603
  49. the transient BMP4 prodomain/ligand complex that forms during sequential cleavage plays an essential role in prodomain-mediated stabilization of the mature ligand PMID: 24993941
  50. BMP4 signaling reduces inflammation and inhibits dysplastic changes in the gastric mucosa after infection of mice with H pylori or H felis. PMID: 24751878
  51. aberrant BMP4-mediated signaling in cranial neural crest cells leads to mis-patterned facial skeleton and congenital bony syngnathia, and suggest an implication of mutations in BMP signaling pathway in human bony syngnathia. PMID: 24785830
  52. Chronic psychological stress activates BMP4-dependent extramedullary erythropoiesis. PMID: 24283209
  53. BMP-4 suppresses kisspeptin-induced GnRH expression by reducing ERK signaling activity. PMID: 23880664
  54. Adult human and mouse hearts express BMP4, the mRNA was increased in patients with coronary artery disease and dilated cardiomyopathy PMID: 24398041
  55. Endogenous Bmp4 in preadipocytes is indispensable for the onset of the adipogenic program, and may help to maintain the preadipocytic state during adipocyte differentiation. PMID: 23911203
  56. Rho-kinase negatively regulates BMP-4-stimulated osteocalcin synthesis via the p38 MAP kinase pathway in osteoblasts. PMID: 24368139
  57. The results support a model in which BMP4 induces MITF expression in pluripotent stem cells and EDN3 subsequently promotes differentiation of these MITF expressing cells along the melanocyte lineage. PMID: 24080013
  58. Inhibition of BMP4/activin receptor-like kinase 3/reactive oxygen species signaling improved endothelial function in diabetic mice through limiting oxidative stress in endothelium. PMID: 24202302
  59. 50 ng/ml BMP-4 seemed to induce mASCs to differentiate into the adipo-lineage. PMID: 23869763
  60. BMP-4 maintains epithelial polarity via intracellular signaling from basolaterally localized BMP receptors. PMID: 23675417
  61. BMP4 is a peripherally-derived factor that may regulate the survival of motor neurons. PMID: 23472198
  62. A novel mechanism of transcription is involved in pancreatic expression of the Bmp4 gene. PMID: 23626735
  63. BMP-4 and TGF-beta1 were expressed in the transitional epithelium, lamina propia and muscularis mucosa. PMID: 23620745
  64. Effects of BMP4 on mouse primordial follicle growth were assayed by follicle count and TUNEL labeling. The expression of phospho-SMAD1/5/8, Sohlh2, and c-kit were measured by immunohistochemistry, RT-PCR, and Western blotting. PMID: 23212987
  65. Msx1 and Tbx2 antagonistically regulate Bmp4 expression during the bud-to-cap stage transition in tooth development. PMID: 23720046
  66. short-term expression of constitutively active Akt in the endothelium of mice results in non-anemic stress erythropoiesis in the spleen PMID: 23383068
  67. COCH is a part of the downstream target network of BMP4 signaling and serves as another important effector to fine-tune mouse embryonic stem cell fates. PMID: 23344953
  68. These findings provide a genetic and metabolic basis for BMP4's role in altering insulin sensitivity by affecting white adipose tissue development. PMID: 23388637
  69. Adding exogenous BMP4 to culture medium could differentiate pluripotent stage-specific embryonic antigen-1+ cells isolated from an adult tissue into gamete precursors, primordial germ cells. PMID: 22988836
  70. we conclude that BMP4 is a mediator and novel therapeutic target for pathological cardiac hypertrophy. PMID: 23248151
  71. In HSPCs, BMP4 directly regulates Integrin-alpha4 expression through SMAD-independent p38 MAPK-mediated signaling. PMID: 23243277
  72. GATA-2 expression in the caudal urogenital mesenchyme as directed by the UG4 enhancer is crucial for proper development of the urinary tract and that its regulation of BMP4 expression is a critical aspect of this function PMID: 22493062
  73. Bmp4 signaling suppresses tooth developmental inhibitors in the tooth mesenchyme, including Dkk2 and Osr2, and synergizes with Msx1 to activate mesenchymal odontogenic potential for tooth morphogenesis and sequential tooth formation PMID: 23250216
  74. study found BMP4 is constitutively expressed in the peribiliary stroma and endothelial cells of the liver and that expression is decreased after hepatectomy; evidence provided that BMP4 signals through Alk3 to inhibit liver regeneration PMID: 23019195
  75. A regulatory loop involving Dies1 and miR-125a controls BMP4 signaling in mouse embryonic stem cells. PMID: 22751012
  76. Antigen presenting cell (APC) candidates from developing mouse cerebellum are characterized in vitro and eventually found to be glial fibrillary acidic protein (GFAP)and bone morphogenetic protein (BMP)4, surviving markers of the original cells. PMID: 21732075
  77. CHD7 may directly regulate Bmp4 expression by binding with an enhancer element downstream of the Bmp4 locus. PMID: 22658483
  78. Pitx homeoprotein family members bind a specific site in the Bmp4 incisor epithelium limb bud enhancer element that is necessary for its activity in vivo. PMID: 22701669
  79. the expression of Zeb1 and Zeb2 in the dental mesenchyme was recovered by Bmp4 signaling PMID: 22350174
  80. Scx and Id1 are involved in the BMP4-Smad1-SMA signal transduction pathway besides the TGFbeta1-Smad1-SMA signaling pathway and modulate phenotypic changes in MCs in diabetic nephropathy. PMID: 22474292
  81. Progenitor cells of the first and second heart fields depend on cardiac-specific transcription factors for their differentiation and Wnt/beta-catenin and Bmp act downstream of Notch/RBPJ at this developmental stage. PMID: 22711842
  82. Heparan sulfate is required for embryonic stem cell pluripotency, in particular lineage specification into mesoderm through facilitation of FGF and BMP signaling. PMID: 22556407
  83. an anti-angiogenic role for BMP4 in laser-induced CNV, mediated by direct inhibition of MMP-9 and indirect inhibition of VEGF. PMID: 22392094
  84. Rubie is the gene mutated in Ecl mice, that it is involved in regulating inner ear expression of Bmp4, and that aberrant Bmp4 expression contributes to the Ecl phenotype. PMID: 22253730
  85. down-regulation of Kruppel-like factor-4 (KLF4) by microRNA-143/145 is critical for modulation of vascular smooth muscle cell phenotype by transforming growth factor-beta and bone morphogenetic protein 4. PMID: 21673106
  86. BMP4 causes endothelial cells (ECs) apoptosis through activation of caspase-3 in a ROS/p38MAPK/JNK-dependent signaling cascade. PMID: 22064324
  87. Provide evidence that CCN3 enhances BMP-4 expression and bone nodule formation in osteoblasts, and that the integrin receptor, ILK, p38, JNK, and AP-1 signaling pathways may be involved. PMID: 21898398
  88. these suggest that cell stretch suppresses the BMP4 induction of MSC adipogenesis potentially via upregulating ERK but not through the downregulation of Smad or p38. PMID: 22266311
  89. Data support the hypothesis that Bmp4 signaling regulates craniofacial skeletal development by balancing self-renewal and differentiation pathways in cranial neural crest progenitors. PMID: 22219353
  90. BMP4 protein expression in the mouse female reproductive tract is of importance in folliculogenesis. PMID: 21810858
  91. BMP4 and laminin synergise to induce visceral endoderm differentiation of XEN cells. PMID: 22027433
  92. presence of myotubes inhibits Bmp4 expression in proliferating myoblasts through transcriptional regulation, although the expression is intrinsically increased with time of culture PMID: 21964441
  93. Different doses of bone morphogenetic protein 4 promote the expression of early germ cell-specific gene in bone marrow mesenchymal stem cells. PMID: 21717271
  94. The objective of these studies was to gain further insight into the molecular relationship between Pax9, Msx1, and Bmp4 in dental mesenchyme. PMID: 21546760
  95. Laser capture microdissection collected clean populations of primary lip and palate epithelial cells destined to fuse. Transcripts of Bmp-4/Alk-3 and Tgfbeta-2/Alk-5 were restricted to epithelial seam of fusion site, and epithelium of the prefusion site. PMID: 21959449
  96. Data show that Bmp4, Bmp7, Gremlin and Twisted gastrulation (Twsg1) are all expressed in the thymus and expression was clearly different for each gene investigated. PMID: 21978004
  97. immature AVC expansion in wkm mutants is rescued by depleting Bmp4, indicating that Tmem2 restricts bmp4 expression to delimit the AVC primordium during cardiac development PMID: 21896629
  98. Data show that both Wnt3a and BMP4 inhibit oligodendrocyte (OL) differentiation in vitro, and that when BMP signalling is blocked, neither Wnt3a nor BMP4 have inhibitory effects on OL differentiation. PMID: 21599637
  99. A conditional gene inactivation approach was applied to test the hypothesis that Bmp4 expressed from the Tbx1 expression domain plays a critical role for normal development of outflow tract and pharyngeal arch arteries. PMID: 21123999
  100. Data suggest that down-regulation of BMP4 expression by TNF in CNV and mechanisms established might be useful for defining novel targets for AMD therapy. PMID: 21411747

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Subcellular Location Secreted, extracellular space, extracellular matrix
Protein Families TGF-beta family
Database Links

KEGG: mmu:12159

STRING: 10090.ENSMUSP00000073720

UniGene: Mm.6813

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