Mouse C-X-C motif chemokine 3(CXCL3) ELISA kit

Code CSB-EL006249MO
Size 96T,5×96T,10×96T
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Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
chemokine (C-X-C motif) ligand 3
Alternative Names
Cxcl3 ELISA Kit; Dcip1 ELISA Kit; Gm1960C-X-C motif chemokine 3 ELISA Kit; Dendritic cell inflammatory protein 1 ELISA Kit
Abbreviation
CXCL3
Uniprot No.
Species
Mus musculus (Mouse)
Sample Types
serum, plasma, tissue homogenates
Detection Range
7.8 pg/mL-500 pg/mL
Sensitivity
1.56 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse CXCL3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %92
Range %90-94
1:2Average %96
Range %92-101
1:4Average %103
Range %101-104
1:8Average %89
Range %84-95
Recovery
The recovery of mouse CXCL3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9189-95
EDTA plasma (n=4)8985-94
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
4002.916 2.845 2.881 2.724
2002.111 2.132 2.122 1.965
1001.314 1.430 1.372 1.215
500.825 0.811 0.818 0.661
250.533 0.512 0.523 0.366
12.50.327 0.334 0.331 0.174
6.250.253 0.264 0.259 0.102
00.152 0.162 0.157
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Mouse CXCL3 ELISA Kit was designed for the quantitative measurement of Mouse CXCL3 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 7.8 pg/mL-500 pg/mL and the sensitivity is 1.56 pg/mL.

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Target Background

Function
(From Uniprot)
Ligand for CXCR2. Has chemotactic activity for neutrophils. May play a role in inflammation and exert its effects on endothelial cells in an autocrine fashion.
Gene References into Functions
  1. The studies revealed that, although overall structural and oligomerization features of CXCL3 and CXCL2 are similar, prominent differences were observed in their surface characteristics, thus implicating a functional divergence. PMID: 28928065
  2. these results have indicated that CXCL3 is a novel adipokine that facilitates adipogenesis in an autocrine and/or a paracrine manner through induction of c/ebpb and c/ebpd. PMID: 27512010
  3. Tis21 induces migration of cerebellar granule neuron precursor cells through Cxcl3, which may represent a novel target for medulloblastoma therapy PMID: 23115191
  4. DCIP-1 interacts with chemokine CXCR2 and mediates human neutrophil chemotaxis, consistent with its role during the early proinflammatory phase of dendritic cell maturation. PMID: 14764687
Subcellular Location
Secreted.
Protein Families
Intercrine alpha (chemokine CxC) family
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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