Mouse C-X-C motif chemokine 3(CXCL3) ELISA kit

Mouse C-X-C motif chemokine 3(CXCL3) ELISA kit

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ELISA Kit
Mouse C-X-C motif chemokine 3(CXCL3) ELISA kit

Protocol

Code	CSB-EL006249MO
Size	96T,5×96T,10×96T
Trial Size
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Description

This Mouse CXCL3 ELISA Kit was designed for the quantitative measurement of Mouse CXCL3 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 7.8 pg/mL-500 pg/mL and the sensitivity is 1.56 pg/mL.

Target Name

chemokine (C-X-C motif) ligand 3

Alternative Names

Cxcl3 ELISA Kit; Dcip1 ELISA Kit; Gm1960C-X-C motif chemokine 3 ELISA Kit; Dendritic cell inflammatory protein 1 ELISA Kit

Abbreviation

CXCL3

Uniprot No.

Q6W5C0

Species

Mus musculus (Mouse)

Sample Types

serum, plasma, tissue homogenates

Detection Range

7.8 pg/mL-500 pg/mL

Sensitivity

1.56 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse CXCL3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	90-94
1:2	Average %	96
	Range %	92-101
1:4	Average %	103
	Range %	101-104
1:8	Average %	89
	Range %	84-95

Recovery

The recovery of mouse CXCL3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	89-95
EDTA plasma (n=4)	89	85-94

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
400	2.916	2.845	2.881	2.724
200	2.111	2.132	2.122	1.965
100	1.314	1.430	1.372	1.215
50	0.825	0.811	0.818	0.661
25	0.533	0.512	0.523	0.366
12.5	0.327	0.334	0.331	0.174
6.25	0.253	0.264	0.259	0.102
0	0.152	0.162	0.157

ELISA Data Analysis

Watch ELISA data processing video & download Curve Expert if needed

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

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Function (From Uniprot)	Ligand for CXCR2. Has chemotactic activity for neutrophils. May play a role in inflammation and exert its effects on endothelial cells in an autocrine fashion.
Gene References into Functions	The studies revealed that, although overall structural and oligomerization features of CXCL3 and CXCL2 are similar, prominent differences were observed in their surface characteristics, thus implicating a functional divergence. PMID: 28928065 these results have indicated that CXCL3 is a novel adipokine that facilitates adipogenesis in an autocrine and/or a paracrine manner through induction of c/ebpb and c/ebpd. PMID: 27512010 Tis21 induces migration of cerebellar granule neuron precursor cells through Cxcl3, which may represent a novel target for medulloblastoma therapy PMID: 23115191 DCIP-1 interacts with chemokine CXCR2 and mediates human neutrophil chemotaxis, consistent with its role during the early proinflammatory phase of dendritic cell maturation. PMID: 14764687
Subcellular Location	Secreted.
Protein Families	Intercrine alpha (chemokine CxC) family
Database Links	KEGG: mmu:330122 STRING: 10090.ENSMUSP00000031326 UniGene: Mm.244289

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Chemokine Receptors – One Kind of Powerful Seven Transmembrane Spanning G Protein-coupled Receptor The Overview of Chemokine ELISA Data Analysis How to Do The Pre-experiment before Your ELISA Experiment Four Types of ELISA 11 tips for choosing your right ELISA kit Do you Often Trouble in These Problems of ELISA? Problems and Solutions in ELISA Experiments

Related pathways

Chemokine signaling pathway TNF signaling pathway IL-17 signaling pathway NOD-like receptor signaling pathway

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St. Ste 610-312, Houston, TX 77054, USA

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.