Mouse C-X-C motif chemokine 9(CXCL9) ELISA kit

Code CSB-EL006252MO
Size 96T,5×96T,10×96T
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Product Details

Target Name
chemokine (C-X-C motif) ligand 9
Alternative Names
Cxcl9 ELISA Kit; Mig ELISA Kit; Scyb9C-X-C motif chemokine 9 ELISA Kit; Gamma-interferon-induced monokine ELISA Kit; Monokine induced by interferon-gamma ELISA Kit; MIG ELISA Kit; MuMIG ELISA Kit; Protein m119 ELISA Kit; Small-inducible cytokine B9 ELISA Kit
Abbreviation
CXCL9
Uniprot No.
Species
Mus musculus (Mouse)
Sample Types
serum, plasma, tissue homogenates
Detection Range
15.6 pg/mL-1000 pg/mL
Sensitivity
3.9 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse CXCL9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 85
Range % 82-89
1:2 Average % 106
Range % 103-109
1:4 Average % 89
Range % 85-93
1:8 Average % 107
Range % 103-110
Recovery
The recovery of mouse CXCL9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 96 93-99
EDTA plasma (n=4) 102 98-106
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
1000 2.445 2.401 2.423 2.287
500 1.938 1.905 1.922 1.786
250 1.206 1.301 1.254 1.118
125 0.667 0.609 0.638 0.502
62.5 0.405 0.394 0.400 0.264
31.2 0.256 0.258 0.257 0.121
15.6 0.209 0.201 0.205 0.069
0 0.137 0.134 0.136  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

The product CSB-EL006252MO is a sandwich ELISA kit developed to measure concentrations of mouse CXCL9 in serum, plasma, or tissue homogenates. This assay uses the sandwich enzyme immunoassay technique in combination with the enzyme-substrate chromogenic reaction to quantify the analyte in the sample. The color develops positively to the amount of CXCL9 in samples. The color intensity is measured at 450 nm via a microplate reader.

CXCL9, also known as MIG, can be produced during inflammatory conditions by myeloid cells within the tumor microenvironment. It attracts CXCR3-expressing cells including activated T and NK cells to the inflammatory environment and plays a role in responses to immune checkpoint therapy. Overexpression of CXCL9 has also been shown to reduce tumor progression and metastasis via the inhibition of angiogenesis. However, CXCL9 can act directly on CXCR3-expressing tumor cells to promote cell migration and epithelial-mesenchymal transition.

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Target Background

Function
(From Uniprot)
May be a cytokine that affects the growth, movement, or activation state of cells that participate in immune and inflammatory response.
Gene References into Functions
  1. although the expression of CXCL9 and CXCL11 are increased after spinal nerve ligation, they may not contribute to the maintenance of neuropathic pain. PMID: 29712506
  2. Cxcl9 has a role in angiogenesis and osteogenesis in bone. PMID: 27966526
  3. Data suggest that the CXCL9-CXCR3 axis plays a pivotal role in the liver-specific distribution of TRAIL+ NK cells in mice. PMID: 29088306
  4. IFNalphaR1 signaling promoted CXCL9 and CXCL10 synthesis, suggesting that these chemokines might be involved in the LPS and CD134 costimulation response. PMID: 28432083
  5. CXCL10 plays in the pathogenesis of recurrent Herpetic stromal keratitis, and that CXCL9 displays its importance when CXCL10 is absent. PMID: 28282568
  6. CXCL9 expression is strongly upregulated in PGRN KO mice and its level is correlated with severity of inflammation in a dermatitis model. PMID: 26892362
  7. hepatic expression of the inflammatory CXC chemokine ligands (CXCL)9 and CXCL10 strongly increased whereas homeostatic CXCL12 significantly decreased. PMID: 26052942
  8. Here, we report the evidence for the production of MIG, a second CXCR3 ligand, during the primary immune response to HSV-1 corneal infection. PMID: 25207638
  9. These findings identify a novel role for the immune cell-derived CXCL9 chemokine in directing a protective antimicrobial response in the intestinal mucosa. PMID: 25643352
  10. These results indicate that CXCL9 is crucial for recruiting immune T cells into the brain and inducing an accumulation of the T cells into the areas where tachyzoites proliferate to prevent reactivation of chronic T. gondii infection. PMID: 25432064
  11. tumours are characterized by expression of inflammatory chemokines (CCL2, CCL5, CCL7, CCL8, CCL12, CXCL9, CXCL10 and CX3CL1), reflected by an enrichment of activated Foxp3(-) and Foxp3(+) T cells PMID: 25495686
  12. Aged mice had similar levels of IL-1beta, TNF, IFN-gamma, IL-17, and granulocyte colony-stimulating factor following S. pneumoniae infection, compared with young mice, but increased levels of the chemokines CXCL9, CXCL12, CCL3, CCL4, CCL5, CCL11, and CCL17. PMID: 25595646
  13. Data indicate that a feed-forward CXCL9-dependent circuit provided additional chemotactic cues that further increase local memory cell density. PMID: 23352234
  14. IFN-gamma-mediated loss of Mig expression in cutaneous tumors as a potent mechanism of immunoediting that results in increased tumor resistance to T cell-mediated immunity. PMID: 23241877
  15. CXCL9/10 have antifibrotic roles on liver non-parenchymal cells PMID: 22905138
  16. findings show that effector T cells cannot accumulate within the decidua, the specialized stromal tissue encapsulating the fetus and placenta; impaired accumulation was in part attributable to the epigenetic silencing of key T cell-attracting inflammatory chemokine genes in decidual stromal cells PMID: 22679098
  17. Mig contributes to the acute lethal toxicity arising from 5-FU administration. PMID: 22474250
  18. Cxcr3, Cxcl9 and Cxcl10 are increased in alopecia areata. PMID: 22358057
  19. The results identify direct angiostatic and antifibrotic effects of the Cxcr3 ligand Cxcl9 in a model of experimental liver fibrosis. PMID: 22237831
  20. MIG/CXCL9 is expressed in the lungs upon pneumococcal infection in a MyD88-dependent manner PMID: 20381636
  21. Expression of the chemokine Mig (CXCL9) was increased 2.8-fold in tumors from Egr-1 knockout mice. PMID: 19200397
  22. CXCL9 promotes the development of IFN-gamma-producing CD8 T cells, and CXCL10 antagonizes this skewing during allograft rejection. PMID: 20194716
  23. CCL2, CXCL9 and CXCL2 mRNA are up-regulated after oral Salmonella infection in Peyer's patches and lymph nodes coincident with the first arrival of monocytes and neutrophils PMID: 19839009
  24. MIG (CXCL9) chemokine gene therapy combines with antibody-cytokine fusion protein to suppress growth and dissemination of murine colon carcinoma. PMID: 11731434
  25. in study of relevance of chemokine expression to selective migration of t-cells and the disease localization in murine graft-versus-host disease, Mig was found to be predominantly expressed in spleen, liver, and not skin, and not heart. PMID: 12098066
  26. NF-kappaB has a critical role in mediating IFN-gamma-induced MIG (monokine induced by IFN-gamma) expression independent of hyaluronan PMID: 12226082
  27. involved in T cell cardiac allograft vasculopathy PMID: 12368204
  28. Data show that the transcriptional coactivator CREB-binding protein (CBP) mediated the STAT1/NF-kappaB synergy for transcription of the gene for CXC ligand 9 an interferon-gamma (IFN-gamma)-inducible chemokine. PMID: 12403783
  29. full potency of SLC/CCL21-mediated anti-tumor responses require in part the induction of IFNgamma, MIG/CXCL9 and IP-10/CXCL10 PMID: 12740040
  30. Peak of expression of CXCL9 and CXCL10 occurred 4 days before CD8+ T cells infiltrated infected tissues. CXCL9 and CXCL10 may play role early during immune response against rickettsial infections. PMID: 14507644
  31. Mig functions as a negative regulator of murine eosinophils PMID: 14769916
  32. exogenous CXCL9 stimulated CD4 lymphocyte proliferation in a MHC class II-mismatched MLR and increased the number of IFN-gamma-producing CD4 lymphocytes PMID: 15187119
  33. Interactions involving CXCR3 and its primary ligands Mig and IP-10 significantly contribute to donor T cell recruitment to the lung after allogeneic stem cell transplantation. PMID: 15265940
  34. RANKL stimulates the serine phosphorylation of STAT1, causing MIG gene transcription and secretion, which may have a role in recruiting CXCR3-positive osteoclast precursors and osteoclasts to bone remodeling or inflammatory sites. PMID: 15585657
  35. IFN-gamma knockout mice, which manifested depressed ear-swelling following delayed hypersensitsivity challenge, made no Mig. PMID: 15629884
  36. Results suggest that in the sensitized host, CXCR3, IP-10, and Mig are required for optimal delayed hypersensitivity responsiveness but are not essential for containing HSV-1 replication. PMID: 15708587
  37. results suggest that MUM1 plays roles in the progression of B-cell lymphoma/leukemia by regulating the expression of various genes including MIG. PMID: 15959530
  38. CXCL9 has a role in graft rejection in the absence of CCL19 and CCL21 PMID: 16095489
  39. MIG mRNA expression in the lungs of Klebsiella-infected mice requires the endogenous production of IFN-gamma. PMID: 16299319
  40. CXCL9 signaling enhances immune responses following Trypanosoma cruzi infection; transcripts for CXCL9 remain elevated during chronic infection PMID: 16368965
  41. CXCL9 is up-regulated in unique patterns following tracheal transplantation in mice. Deletion of CXCL9 does not affect airway obliteration. PMID: 16709871
  42. These results suggest that the more aggressive rejection of xenografts compared with allografts is due to the earlier expression of CXC-chemokines, IP-10 and MIG, and subsequent adjuvant effects of proinflammatory cytokines. PMID: 16768726
  43. Collectively, the results suggest a non-redundant role for CXCL9 and CXCL10 in response to ocular HSV-1 infection in terms of controlling virus replication and recruitment of CD4(+) T cells into the cornea. PMID: 17296171
  44. Acute ethanol intoxication impairs lung expression of Cxcl9, interfering with pulmonary response to bacterial challenge. PMID: 17889309
  45. IFN-gamma is mediator of Cxcl10 and Cxcl9 gene expression in experimental autoimmune encephalomyelitis(EAE). It differentially regulates expression of these genes by astrocytes and microglia. Differential glial localization of these chemokines in EAE. PMID: 17902170
  46. The absence of CXCL9 or CXCL10 expression significantly alters the ability of the host to control genital HSV-2 infection through the mobilization of effector cells to sites of infection. PMID: 18178850
  47. These data demonstrate that CXCR3 on CD8(+) T cells is required for T cell recruitment into the brain and the development of murine cerebral malaria. PMID: 18347328
  48. Liver sinusoidal endothelial cells present chemokines (CXCL12 and CXCL9) to circulating lymphocytes. PMID: 18697212
  49. CXCR3 ligands, IP10 and MIG, contribute to Th1-induced inflammation but not to homing of Th1 cells into the lung. PMID: 18716926
  50. CXCL9 promotes protection from coronavirus-induced neurological and liver disease. PMID: 18973912

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Subcellular Location
Secreted.
Protein Families
Intercrine alpha (chemokine CxC) family
Database Links

KEGG: mmu:17329

STRING: 10090.ENSMUSP00000108716

UniGene: Mm.766

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