24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now
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|Intra-assay Precision (Precision within an assay): CV%<8%|
|Three samples of known concentration were tested twenty times on one plate to assess.|
|Inter-assay Precision (Precision between assays): CV%<10%|
|Three samples of known concentration were tested in twenty assays to assess.|
|To assess the linearity of the assay, samples were spiked with high concentrations of mouse CXCL9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.|
|The recovery of mouse CXCL9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.|
|Sample Type||Average % Recovery||Range|
|EDTA plasma (n=4)||102||98-106|
|These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.|
The product CSB-EL006252MO is a sandwich ELISA kit developed to measure concentrations of mouse CXCL9 in serum, plasma, or tissue homogenates. This assay uses the sandwich enzyme immunoassay technique in combination with the enzyme-substrate chromogenic reaction to quantify the analyte in the sample. The color develops positively to the amount of CXCL9 in samples. The color intensity is measured at 450 nm via a microplate reader.
CXCL9, also known as MIG, can be produced during inflammatory conditions by myeloid cells within the tumor microenvironment. It attracts CXCR3-expressing cells including activated T and NK cells to the inflammatory environment and plays a role in responses to immune checkpoint therapy. Overexpression of CXCL9 has also been shown to reduce tumor progression and metastasis via the inhibition of angiogenesis. However, CXCL9 can act directly on CXCR3-expressing tumor cells to promote cell migration and epithelial-mesenchymal transition.
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