Mouse Cathepsin B(CTSB) ELISA kit

Code CSB-EL006185MO
Size 96T,5×96T,10×96T
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Product Details

Target Name
cathepsin B
Alternative Names
Ctsb ELISA Kit; Cathepsin B ELISA Kit; EC ELISA Kit; Cathepsin B1) [Cleaved into: Cathepsin B light chain; Cathepsin B heavy chain] ELISA Kit
Uniprot No.
Mus musculus (Mouse)
Sample Types
serum, plasma, tissue homogenates, cell lysates
Detection Range
15.6 pg/mL-1000 pg/mL
3.9 pg/mL
Assay Time
Sample Volume
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of mouse CTSB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 91
Range % 83-95
1:2 Average % 98
Range % 92-103
1:4 Average % 96
Range % 85-100
1:8 Average % 103
Range % 92-107
The recovery of mouse CTSB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 94 91-97
EDTA plasma (n=4) 100 92-104
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
1000 2.628 2.532 2.580 2.474
500 2.315 2.189 2.252 2.146
250 1.856 1.776 1.816 1.710
125 1.124 1.102 1.113 1.007
62.5 0.758 0.734 0.746 0.640
31.2 0.472 0.463 0.468 0.362
15.6 0.315 0.302 0.309 0.203
0 0.104 0.108 0.106  
and FAQs
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days

This Mouse CTSB ELISA Kit was designed for the quantitative measurement of Mouse CTSB protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.

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Target Background

(From Uniprot)
Thiol protease which is believed to participate in intracellular degradation and turnover of proteins. Cleaves matrix extracellular phosphoglycoprotein MEPE. Involved in the solubilization of cross-linked TG/thyroglobulin in the thyroid follicle lumen. Has also been implicated in tumor invasion and metastasis.
Gene References into Functions
  1. Compared to WT leukocytes, CtsB(-/-) leukocytes accumulated in a higher number in angiogenic vessels, but extravasated less toward the implanted cytokine. The accumulated CtsB(-/-) leukocytes in angiogenic vessels expressed more CD18. PMID: 28904019
  2. CatB aggravates Coxsackievirus B3-induced viral myocarditis(VMC) probably through activating the inflammasome and promoting pyroptosis. This finding might provide a novel strategy for VMC treatment. PMID: 29360865
  3. Activation of the proteases in macrophage occurs during endocytosis of zymogen-containing vesicles, and depends on pH and CTSB in acute necrotizing pancreatitis. PMID: 29079517
  4. Acid ceramidase inhibitor LCL521 targets lysosomes to activate cathepsin B and cathepsin D, resulting in interrupted autophagy and ER stress that culminates in myeloid-suppressor cell death. PMID: 27880732
  5. Using quenched activity-based probes, we identified mouse cathepsin B (CtsB), which is expressed in the lower regions of the hair follicle, as an additional target of mouse Cst6 PMID: 28596234
  6. After phagocytosis, particles can disrupt lysosomes, and inhibitor studies have suggested that the resulting release of a lysosomal protease-cathepsin B-into the cytosol somehow activates NLRP3. PMID: 28087651
  7. ctsB enhanced neutrophil migration though a putative effect on pseudopod retraction rates. PMID: 28389621
  8. the roles of CTSB/S and SIRT1 in the regulation of hepatic inflammation using primary parenchymal and non-parenchymal hepatic cell types and cell lines. PMID: 27831566
  9. Mice and macrophages lacking cathepsin B activity had increased resistance to the cytosolic bacterial pathogen Francisella novicida Genetic deletion. PMID: 27551156
  10. in CTSB knockout (KO) mice, running did not enhance adult hippocampal neurogenesis and spatial memory function. Interestingly, in Rhesus monkeys and humans, treadmill exercise elevated CTSB in plasma. PMID: 27345423
  11. Exposure of J774A.1 cells to HOCl or HOSCN resulted in a significant decrease in the activity of the Cys-dependent cathepsins B and L, but not the Asp-dependent cathepsin D. PMID: 27997605
  12. cathepsin B (CTSB) inhibition or expression of a CTSB-resistant Dab2 mutant maintains Dab2 expression and shifts long-term TGF-beta-treated cells from autophagy to apoptosis PMID: 27398911
  13. study brings forth a yet unappreciated role for Cathepsin B in regulating T cell responses during Leishmania major infection PMID: 27182703
  14. Our results suggest that the Mucopolysaccharidosis type I mice have progressive heart failure and valve disease, which may be caused by cathepsin B overexpression. PMID: 28104572
  15. CTSB in pancreatitis undergoes activation in a secretory, vesicular, and acidic compartment where it activates trypsinogen. Its deletion or inhibition regulates acinar cell apoptosis but not necrosis in two models of pancreatitis PMID: 27226576
  16. IGF-1 promotes the growth and metastasis of hepatocellular carcinoma by inhibition of proteasome-mediated cathepsin B degradation. PMID: 26401078
  17. We therefore conclude that CD in pancreatic acinar cells is implicated in CB and CL degradation but not in autophagic activity. PMID: 26682926
  18. Aggregatibacter actinomycetemcomitans invasion of macrophages induces interleukin-1beta production through reactive oxygen species and cathepsin B. PMID: 25789553
  19. Findings indicate that Ctsb-/- BMDC display more pro-Th1 properties than their WT and Ctsl-/- counterparts, and therefore suggest that Ctsb down-regulates the Th1 response to L. major. PMID: 25255101
  20. Cathepsin B showed the highest protein levels in the anterior segments of the gastrointestinal tract, whereas the highest activity was observed in the jejunum PMID: 25205730
  21. CtsB and CtsL are essential in alpha-syn lysosomal degradation PMID: 26170293
  22. Reactive oxygen species and cathepsin B activities are necessary for maximal iNOS induction in C. muridarum-infected macrophages. PMID: 26015483
  23. CTSB protein functions as a necessary modulator of hypertrophic response by regulating TNF-alpha/ASK1/JNK signaling pathway involved in cardiac remodeling. PMID: 25713304
  24. Abeta oligomers, but not fibrils, induce IL-1beta production in primary microglia in a cathepsin B- and reactive oxygen species -dependent manner. PMID: 25680460
  25. data illustrate the role of CatB in producing pGlu-Abeta and flAbeta that participate as key factors in the development of Alzheimer's disease PMID: 24595198
  26. cysteine cathepsins B and S can directly cleave Rip1 PMID: 24799565
  27. The increase in cathepsins B and L activities in lymphosarcoma tissues is caused by cyclophosphamide induction of apoptosis. PMID: 24319737
  28. Cathepsin B produces full-length amyloid-beta (Ab(1-40/42)) and pyroglutamate amyloid-beta (pyroGluAb(3-40/42) and is a key drug target for treating Alzheimer's disease by reducing these amyloid-beta species PMID: 24595198
  29. High cathepsin expression is associated with dysplasia and colitis-associated cancer. PMID: 23591598
  30. High cathepsin B expression is associated with glioma. PMID: 22495828
  31. Cathepsin B is a novel gender-dependent determinant of cholesterol absorption from the intestine. PMID: 23248330
  32. Results indicate influence of both Ctsb and Ctsl on the skin proteome and degradome, with the phenotypic consequences of the absence of either protease differing considerably. PMID: 23233448
  33. Cathepsin-mediated necrosis controls the adaptive immune response by Th2 (T helper type 2)-associated adjuvants PMID: 23297415
  34. These results show that CatB and CatL activity is not required for ebola virus replication. PMID: 23236527
  35. Relative position of thyroglobulin (Tg)peptide epitopes are identified based on homology to known Ctsb cleavage sites in rabbit Tg. PMID: 23315080
  36. Enhancing neuronal CatB activity in hAPP(WT) mice significantly lowered Abeta42 levels. PMID: 23024364
  37. We demonstrate that ATP, acting through the P2X7 receptor, induces release of cathepsin B into the extracellular space where it degrades TIMP-1, permitting the MMP-9-dependent migration of glial cells. PMID: 23017058
  38. This study provided that initiation animal disease model peripheral inflammation-induced chronic pain contribute by Microglial cathepsin B. PMID: 22895716
  39. Data suggest that during development of obesity, lipotoxic lipids trigger Ctsb-dependent lysosomal permeabilization in adipocytes; Ctsb induces mitochondrial dysfunction, ROS production, cell death, and recruitment of macrophages into adipose tissue. PMID: 22739104
  40. up-regulation of endothelial CTSB due to Fli1 deficiency may contribute to the development of SSc vasculopathy PMID: 22384200
  41. Knockout of the CatB gene in the AbetaPPWT/Lon mice improved memory deficits and altered the pattern of Abeta-related biomarkers in a manner consistent with CatB having WT beta-secretase activity. PMID: 22337825
  42. plays an important cell autonomous role in the progression of pancreatic ductal adenocarcinoma PMID: 22157328
  43. between mRNA expression and activity of beta-secretase, cathepsin D, and cathepsin B in the brain of SAMR1 and SAMP8 with age. PMID: 22008266
  44. A rise in lysosomal pH has been proposed as the trigger of ATP-dependent lysosomal secretion of cathepsin B in this murine pneumococcal meningitis model. PMID: 22003197
  45. Ctsb has significant effects on emotionality PMID: 21897848
  46. Findings point to a role for cathepsin D and B in cell debris degradation after apoptotic processes rather than promoting cell death, as proposed for other developmental models. PMID: 21648018
  47. The expression of cathepsins D and B in the murine ectoplacental cone was correlated with the ultrastructural progress of decidual invasion by trophoblast cells. PMID: 21116106
  48. data suggest that subcellular redistribution of cathepsin B, in itself, induces neither spontaneous trypsinogen activation nor pancreatitis PMID: 21081800
  49. Results suggest P2X(7)R activation may play a novel and direct role in tissue damage through release of cathepsins. PMID: 20639492
  50. These observations strongly suggest that CB plays a critical role in axotomy-induced mortor neuron death in adult mice. PMID: 20673755

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Subcellular Location
Lysosome. Melanosome. Secreted, extracellular space. Apical cell membrane; Peripheral membrane protein; Extracellular side.
Protein Families
Peptidase C1 family
Tissue Specificity
Expressed in thyroid epithelial cells.
Database Links
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