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| Code | CSB-E05145m |
| Size | 96T,5×96T,10×96T |
| Price | Request a Quote or Start an on-line Chat |
| Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse ET-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 87 | ||||
| Range % | 83-94 | |||||
| 1:2 | Average % | 93 | ||||
| Range % | 89-98 | |||||
| 1:4 | Average % | 94 | ||||
| Range % | 89-100 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 88-99 | |||||
| The recovery of mouse ET-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 92-102 | ||||
| EDTA plasma (n=4) | 98 | 90-105 | ||||
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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CUSABIO's Endothelin-1 (ET-1) mouse ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse ET-1 in serum, plasma, or cell culture supernates. This assay uses an antibody specific for mouse ET-1 coated on a 96-well plate. ET-1 present in a sample is bound to the wells by the immobilized antibody after adding the sample into the wells. Biotinylated ET-1 antibody is added to the wells, forming an antibody-analyte-antibody complex. HRP-conjugated avidin is pipetted to the wells and binds to the complex. A TMB substrate solution is added to the wells and color develops in proportion to the amount of ET-1 bound. The addition of Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm using a microplate reader.
ET-1, also called EDN1, is a powerful endogenous vasoconstrictor and is mainly secreted by endothelial cells. In addition to the vasoconstrictive action, ET-1 also leads to fibrosis of the vascular cells and induces the generation of reactive oxygen species (ROS), especially superoxide anions, thus resulting in the development of oxidative stress. ET-1 also exerts a pro-inflammatory effect. It increases the expression of adhesion molecules on vascular endothelial cells and stimulates the aggregation of polymorphonuclear neutrophils contributing to inflammation and endothelial dysfunction.
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