Mouse Endothelin 1,ET-1 ELISA Kit

Code CSB-E05145m
Size 96T,5×96T,10×96T How to order?
Trial Size 24T ELISA kits trial application
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Product Details

Description

CUSABIO's Endothelin-1 (ET-1) mouse ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse ET-1 in serum, plasma, or cell culture supernates. This assay uses an antibody specific for mouse ET-1 coated on a 96-well plate. ET-1 present in a sample is bound to the wells by the immobilized antibody after adding the sample into the wells. Biotinylated ET-1 antibody is added to the wells, forming an antibody-analyte-antibody complex. HRP-conjugated avidin is pipetted to the wells and binds to the complex. A TMB substrate solution is added to the wells and color develops in proportion to the amount of ET-1 bound. The addition of Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm using a microplate reader.

ET-1, also called EDN1, is a powerful endogenous vasoconstrictor and is mainly secreted by endothelial cells. In addition to the vasoconstrictive action, ET-1 also leads to fibrosis of the vascular cells and induces the generation of reactive oxygen species (ROS), especially superoxide anions, thus resulting in the development of oxidative stress. ET-1 also exerts a pro-inflammatory effect. It increases the expression of adhesion molecules on vascular endothelial cells and stimulates the aggregation of polymorphonuclear neutrophils contributing to inflammation and endothelial dysfunction.

Target Name endothelin 1
Alternative Names Edn1Endothelin-1 ELISA kit; ET-1 ELISA kit; Preproendothelin-1 ELISA kit; PPET1) [Cleaved into: Big endothelin-1] ELISA kit
Abbreviation EDN1
Uniprot No. P22387
Species Mus musculus (Mouse)
Sample Types serum, plasma, cell culture supernates
Detection Range 15.6 pg/mL-1000 pg/mL
Sensitivity 3.9 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cancer
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse ET-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %87
Range %83-94
1:2Average %93
Range %89-98
1:4Average %94
Range %89-100
1:8Average %95
Range %88-99
Recovery
The recovery of mouse ET-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9692-102
EDTA plasma (n=4)9890-105
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
10002.729 2.612 2.671 2.524
5001.938 1.876 1.907 1.760
2501.228 1.204 1.216 1.069
1250.816 0.759 0.788 0.641
62.50.435 0.407 0.421 0.274
31.20.299 0.275 0.287 0.140
15.60.241 0.223 0.232 0.085
00.151 0.143 0.147  
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
Endothelins are endothelium-derived vasoconstrictor peptides. Probable ligand for G-protein coupled receptors EDNRA and EDNRB which activates PTK2B, BCAR1, BCAR3 and, GTPases RAP1 and RHOA cascade in glomerular mesangial cells.
Gene References into Functions
  1. Knocking down basolateral amygdala (BLA) ET1 expression enhanced anxiety-like behaviors, whereas over-expressing ETBRs, but not A-type receptors (ETARs), had an anxiolytic effect PMID: 28539637
  2. Increased ROS, notably H2O2, contributes to enhanced afferent arteriolar responses to ET-1 in diabetes, which is closely associated with Wnt signaling. PMID: 29870994
  3. Endothelin-1 expression is increased in a model of kidney injury, along with fibroblast expansion. PMID: 29089036
  4. strial capillary basement membrane thickening results in hypoxic stress further elevating extracellular matrix and ET-1 gene expression, exacerbating strial pathology PMID: 27553900
  5. TWEAK may stimulate endothelial ET-1 synthesis, through the up-regulation of ECE-1. PMID: 28025386
  6. c-Src tyrosine kinase mediates high glucose-induced endothelin-1 expression PMID: 27102411
  7. SIX1 regulates dorsal arch development not only by inducing dorsal Jag1 expression but also by inhibiting endothelin 1 (Edn1) expression in the pharyngeal endoderm of the dorsal arch, thus preventing dorsal EDNRA signaling. PMID: 28455376
  8. NOX4-derived reactive oxygen species in general, and possibly superoxide in particular, are involved in flow-stimulated inner medullary collecting duct ET-1 production. PMID: 28515175
  9. in normal tissues the tandem of serine carboxypeptidases, Scpep1 and CathA likely constitutes an important part of the physiological mechanism responsible for the balanced elimination of heightened levels of ET-1 that otherwise would accumulate in tissues and consequently contribute to development of the hyper-proliferative corneal dystrophy and abnormal skin thickening PMID: 28234994
  10. Newborn endothelial cells express high levels of ET-1 peptides, rapidly release ET-1 in response to endothelial stimulation, and initiate ET-1-mediated endothelium-dependent constriction PMID: 27062279
  11. cellular and molecular mechanisms of ET-1 action in periodontitis PMID: 28030574
  12. Taken together, these data indicate that during high-salt feeding, the autocrine actions of ET-1 via upregulation of the ETB receptor are critical for IMCD NO production, facilitating inhibition of ion reabsorption. PMID: 27280426
  13. reduced lung levels of PPARgamma and increased levels of microRNA-27a (miR-27a), v-ets avian erythroblastosis virus E26 oncogene homolog 1 (ETS1), endothelin-1 (ET-1), and markers of endothelial dysfunction (platelet/endothelial cell adhesion molecule 1 and E selectin). PMID: 27612006
  14. Increased circulating Edn1 and expression of Ednra in endothelial cells are characteristic of diabetic kidney disease-susceptible mice. PMID: 27899487
  15. Review and Meta-Analysis of ET-1 Transgenic Mice provides robust evidence that global ET-1 overexpression in mice lowers blood pressure in an age-dependent manner. Older ET-1+/+ mice have a somewhat more pronounced reduction of blood pressure. PMID: 27832660
  16. evidence supports a model in which aldosterone activation of the mineralocorticoid receptor (MR) results in the MR-hormone complex binding at HRE at -671bp to open chromatin structure around other regulatory elements in the Edn1 gene PMID: 26546722
  17. Decreased ET-1 levels were associated with greater activation of NLRP3 and IL-1beta in normal glucose. High glucose increased NLRP3 markers and activation compared to normal and low glucose PMID: 26883974
  18. AMPAR-mediated glutamatergic neurotransmission may underlie the mechanism of ET1-ET(A)R signaling pathway in the regulation of anxiety. PMID: 25899174
  19. indicate that endothelin-1 is critical in the development of cerebrovascular and cognitive impairments with experimental cerebral malaria PMID: 27031954
  20. increased extrarenal vascular ET-1 production in response to HS intake is mediated by increased extracellular osmolarity and plays a critical role in regulating skin storage of Na(+) PMID: 26268928
  21. PPARgamma regulates miR-98 to modulate ET-1 expression and pulmonary endothelial cell proliferation in pulmonary hypertension. PMID: 26098770
  22. these results indicated that I/R induced upregulation of ET1 and ETA in the kidneys, which was, at least in part, dependent on the production of inflammatory cytokines. PMID: 25572710
  23. we have demonstrated that the expression of ET-1 and IL-25 was coordinately upregulated in the lesional keratinocytes of patients with AD and a murine AD model. PMID: 25903653
  24. The cardiac expression of prepro-endothelin-1 mRNA was increased in 18-week-old obese C57BL/6 mice compared to animals with normal weight. Furthermore, endothelin-1 plasma levels showed an increasing trend. PMID: 25181417
  25. This study showed a novel SIRT1 mediated protection against renal and retinal injury in diabetes, regulated through p300, ET-1 and TGF-beta1. PMID: 25753689
  26. these results mechanistically link the innate immune response mediated by IkappaBbeta/NF-kappaB to ET-1 expression and potentially reveal therapeutic targets for patients with Gram-negative septic shock. PMID: 26342031
  27. ET-1 overexpressing astrocytic cells showed amyloid secretion after hypoxia/ischemia insult suggests a role of astrocytic ET-1 in dementia associated with stroke by astrocyte-derived amyloid production PMID: 26104290
  28. These results demonstrated that enhanced endothelin system activity could be a causative factor in the development of PAH and provided rationale for the inhibition of endothelin system to treat PAH. PMID: 26275708
  29. aging-related cardiac fibrosis is, at least partially, dependent on the upregulation of ET-1. PMID: 25584774
  30. ET/ETA signaling plays a crucial role in IL-17 production by Th17. PMID: 24530289
  31. Venous Edn1 guides stellate ganglia axons to innervate the developing heart via Ednra receptors on those axons. Edn1-Ednra signalling is essential for functional regulation of the heart by sympathetic nerves. PMID: 24875861
  32. Edn1 was shown to interact with Argonaute protein suggesting that it is a target of the RNA induced silencing complex (RISC). PMID: 24632479
  33. These results indicate that the cardiac phenotypes of ET-1 transgenic mice and eNOS knockout mice are sex specific. PMID: 24355292
  34. The dependence on feeding schedule and biological clock of the regulation of ET-1 gene expression in mouse colon, was investigated. PMID: 25010841
  35. The role of interleukin-6 (IL-6), and ET signaling in advanced pulmonary arterial hypertension progression, was examined. PMID: 24412382
  36. The present study was designed to investigate whether or not overexpression of endogenous ET-1 in the endothelium affects the responsiveness of the main and segmental renal arteries differently in obesity. PMID: 24412387
  37. endothelin-1 is critical for maintaining normal contractile function, for controlling superoxide and Mmp9 levels. PMID: 25848038
  38. synthesis in inner medullary collecting duct stimulated by flow, which engages Ca(2+)-dependent signaling pathways via polycystin-2 and P2 receptors PMID: 25587122
  39. The observation that loss of ET-1 inhibited SMC proliferation, survival, and migration represents evidence that ET-1 derived from SMC plays a previously undescribed role in modulating the response of the pulmonary circulation to hypoxia. PMID: 25399435
  40. The combined effects of Trypanosoma cruzi and ET-1 on atrial myocytes promoted the protein expression, phosphorylation and DNA-binding activity of GATA4, leading to augmented protein levels of iNOS and increased nitric oxide release. PMID: 24927936
  41. the ET-1/TGF-beta1 axis has a role as an inducer of MMT and subsequent peritoneal damage and fibrosis PMID: 25012164
  42. endothelial cell-derived ET-1 is the potent therapeutic target in fibrosis or wound healing, and investigations of the overall regulatory mechanisms of these pathological conditions by ET-1 may lead to a new therapeutic approach PMID: 24853267
  43. Here, authors have shown that Mycobacterium tuberculosis produces and secretes an enzyme with ET-1 cleavage activity. PMID: 25267836
  44. our results define the biological role of mammalian serine carboxypeptidase Scpep1 and suggest that Scpep1 and CathA together participate in the control of ET-1 regulation of vascular tone and hemodynamics PMID: 24586188
  45. VEGF inhibition augments the pressor response to ET-1 PMID: 24632840
  46. endothelin-1 is required for normal heart function after chronic pressure overload in mice PMID: 24523936
  47. Endothelin-1 drives development of glomerulosclerosis and podocyte loss through direct activation of endothelin receptors and NF-kappaB and beta-catenin pathways in podocytes PMID: 24722437
  48. Endothelin-1 directs airway remodeling and hyper-reactivity in a murine asthma model. PMID: 24117726
  49. Toll-like receptor 2 signaling is suppressed by endothelin-1 in osteoblasts, which may play a role in bone metabolism during infection PMID: 24700498
  50. Endothelin-1 promotes podocyte migration via endothelin-A receptor activation and increased beta-arrestin-1 expression. PMID: 24371298

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Subcellular Location Secreted.
Protein Families Endothelin/sarafotoxin family
Tissue Specificity Highest expression in the adult is in lung. Lower levels found in heart, kidney, brain and intestine. In the embryo, expressed in outer and inner pharyngeal arch surfaces. Also expressed in endothelium of dorsal aorta and arch arteries, and in epithelium
Database Links

KEGG: mmu:13614

STRING: 10090.ENSMUSP00000021796

UniGene: Mm.14543

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