Mouse High mobility group protein B1,HMGB-1 ELISA Kit

Code CSB-E08225m
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P63158
Lead Time 3-5 working days
Abbreviation HMGB1
Target Name high-mobility group box 1
Alias DKFZp686A04236, HMG1, HMG3, SBP-1, Amphoterin|OTTHUMP00000018196|OTTHUMP00000190860|OTTHUMP00000200117|Sulfoglucuronyl carbohydrate binding protein|high mobility group box 1|high mobility group prot
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 15.6 pg/mL-1000 pg/mL
Sensitivity 3.9 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Epigenetics and Nuclear Signaling
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse HMGB-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:100 Average % 92  
Range % 80-98  
1:200 Average % 85  
Range % 81-96  
1:400 Average % 97  
Range % 90-104  
1:800 Average % 95  
Range % 82-98  
Recovery
The recovery of mouse HMGB-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 97 95-100  
EDTA plasma (n=4) 95 90-102  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
1000 2.756 2.712 2.734 2.565  
500 2.223 2.251 2.237 2.068  
250 1.547 1.572 1.560 1.391  
125 1.020 0.986 1.003 0.834  
62.5 0.598 0.621 0.610 0.441  
31.2 0.452 0.432 0.442 0.273  
15.6 0.287 0.275 0.281 0.112  
0 0.173 0.164 0.169    
References
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Function Multifunctional redox sensitive protein with various roles in different cellular compartments. In the nucleus is one of the major chromatin-associated non-histone proteins and acts as a DNA chaperone involved in replication, transcription, chromatin remodeling, V(D)J recombination, DNA repair and genome stability. Proposed to be an universal biosensor for nucleic acids. Promotes host inflammatory response to sterile and infectious signals and is involved in the coordination and integration of innate and adaptive immune responses. In the cytoplasm functions as sensor and/or chaperone for immunogenic nucleic acids implicating the activation of TLR9-mediated immune responses, and mediates autophagy. Acts as danger associated molecular pattern (DAMP) molecule that amplifies immune responses during tissue injury. Released to the extracellular environment can bind DNA, nucleosomes, IL-1 beta, CXCL12, AGER isoform 2/sRAGE, lipopolysaccharide (LPS) and lipoteichoic acid (LTA), and activates cells through engagement of multiple surface receptors. In the extracellular compartment fully reduced HMGB1 (released by necrosis) acts as a chemokine, disulfide HMGB1 (actively secreted) as a cytokine, and sulfonyl HMGB1 (released from apoptotic cells) promotes immunological tolerance
Subcellular Location Nucleus, Cytoplasm, Secreted, Chromosome, Cell membrane, Peripheral membrane protein, Extracellular side, Endosome, Endoplasmic reticulum-Golgi intermediate compartment
Protein Families HMGB family
Tissue Specificity Serum levels are found elevated in mice with modeled systemic lupus erythematosus (SLE) and are correlated with SLE disease activity (PubMed:26078984).
Database Links

KEGG: mmu:100862258

STRING: 10090.ENSMUSP00000082682

UniGene: Mm.207047

Pathway Autophagy
Necroptosis
DNA repair pathway

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