Mouse Immunoglobulin A, IgA ELISA Kit

Code CSB-E07986m
See More Details
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Lead Time 3-5 working days
Abbreviation IgA
Target Name Immunoglobulin A, IgA
Species Mus musculus (Mouse)
Sample Types serum, plasma
Detection Range 25 ng/mL-1600 ng/mL
Sensitivity 25 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse IgA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:5000 Average % 93  
Range % 88-95  
1:10000 Average % 95  
Range % 90-99  
1:20000 Average % 89  
Range % 85-94  
1:40000 Average % 97  
Range % 91-102  
Recovery
The recovery of mouse IgA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 93 89-97  
EDTA plasma (n=4) 102 98-106  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average    
1600 0.352 0.375 0.364    
400 0.490 0.484 0.487    
200 0.618 0.661 0.640    
100 0.786 0.772 0.779    
25 1.005 1.088 1.047    
0 1.834 1.900 1.867    
Still Have Questions? Leave a Message or Start an on-line Chat

Related Products

IgA ELISA Kit

IgA ELISA Kit for Gallus gallus (Chicken)

IgA ELISA Kit for Homo sapiens (Human)

IgA ELISA Kit for Sus scrofa (Pig)

IgA ELISA Kit for Rattus norvegicus (Rat)

IgA ELISA Kit for Ovis aries (Sheep)

IgA ELISA Kit for Bos taurus (Bovine)

IgA ELISA Kit for Capra hircus (Goat)

IgA ELISA Kit for Guinea Sus scrofa (Pig)

IgA ELISA Kit for Oryctolagus cuniculus (Rabbit)

IgA ELISA Kit for Monkey

IgA ELISA Kit for Canis lupus familiaris (Dog) (Canis familiaris)

Most popular with customers

Newsletters

Get all the latest information on Events, Sales and Offers. Sign up for newsletter today.

Copyright © 2007-2018 www.cusabio.com CUSABIO TECHNOLOGY LLC All Rights Reserved.

Wait!

Join the 25,000 subscribers to get research hotpots, technical tips, latest information on events, sales and offers.

Sign up now!

We don't deal in spam.