Mouse Interleukin 13,IL-13 ELISA Kit

Code CSB-E04602m
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P20109
Lead Time 3-5 working days
Abbreviation IL13
Target Name interleukin 13
Alias ALRH, BHR1, IL-13, MGC116786, MGC116788, MGC116789, P600,
Species Mus musculus (Mouse)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 31.25 pg/mL-2000 pg/mL
Sensitivity 7.8 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This gene encodes an immunoregulatory cytokine produced primarily by activated Th2 cells. This cytokine is involved in several stages of B-cell maturation and differentiation. It up-regulates CD23 and MHC class II expression, and promotes IgE isotype switching of B cells. This cytokine down-regulates macrophage activity, thereby inhibits the production of pro-inflammatory cytokines and chemokines. This cytokine is found to be critical to the pathogenesis of allergen-induced asthma but operates through mechanisms independent of IgE and eosinophils. This gene, IL3, IL5, IL4, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL4.
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-13 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 94  
Range % 88-100  
1:2 Average % 92  
Range % 82-98  
1:4 Average % 92  
Range % 85-99  
1:8 Average % 95  
Range % 89-99  
Recovery
The recovery of mouse IL-13 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 96 90-103  
EDTA plasma (n=4) 92 87-96  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
2000 2.906 2.846 2.876 2.704  
1000 2.374 2.363 2.369 2.197  
500 1.595 1.620 1.608 1.436  
250 0.896 0.867 0.882 0.710  
125 0.523 0.558 0.541 0.369  
62.5 0.381 0.394 0.388 0.216  
31.25 0.271 0.257 0.264 0.092  
0 0.176 0.167 0.172    
References
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Function Cytokine. Inhibits inflammatory cytokine production. Synergizes with IL2 in regulating interferon-gamma synthesis. May be critical in regulating inflammatory and immune responses (By similarity). Positively regulates IL31RA expression in macrophages
Subcellular Location Secreted
Protein Families IL-4/IL-13 family
Database Links

KEGG: mmu:16163

STRING: 10090.ENSMUSP00000020650

UniGene: Mm.1284

Pathway Jak-STAT signaling pathway
Fc epsilon RI signaling pathway
IL-17 signaling pathway
Th1 and Th2 cell differentiation

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