Recombinant Mouse Interleukin-13 protein (Il13) is expressed in E. coli, covering the amino acid region M+22-131aa and is tag-free. This partial protein features a purity greater than 97% as determined by SDS-PAGE and maintains an endotoxin level of less than 1.0 EU/μg using the LAL method. It is fully biologically active, with an ED50 of less than 4 ng/ml in a cell proliferation assay with human TF-1 cells, indicating a specific activity greater than 2.5 × 10^5 IU/mg.
Interleukin-13 (IL-13) appears to be a key cytokine in immune response modulation. The protein plays what seems to be a critical role in regulating inflammation and tissue remodeling processes. IL-13 likely influences various signaling pathways that are associated with immune cell differentiation and activation, which may explain why it has become such an important target in immunological research. For scientists studying allergic responses and other immune-related conditions, understanding how IL-13 functions appears essential.
Potential Applications
Note: The applications listed below are based on what we know about this protein's biological functions, published research, and experience from experts in the field. However, we haven't fully tested all of these applications ourselves yet. We'd recommend running some preliminary tests first to make sure they work for your specific research goals.
1. Cell Proliferation and Viability Assays
This recombinant mouse IL-13 protein works well for stimulating cell proliferation in responsive cell lines, particularly when researchers want to dig into cytokine signaling pathways. With its demonstrated biological activity showing an ED50 of less than 4 ng/ml in TF-1 cells, it offers a dependable tool for dose-response studies. Scientists can harness this protein to investigate IL-13-mediated cellular responses - things like proliferation kinetics and the downstream signaling cascades that follow. The high purity (>97%) combined with low endotoxin levels makes it particularly suitable for sensitive cell culture experiments where even minor contamination might throw off results.
2. Cytokine Receptor Binding Studies
The biologically active mouse IL-13 protein serves as an effective ligand in receptor binding assays, especially when the goal is studying IL-13 receptor interactions and binding kinetics. Scientists often turn to this protein for competitive binding experiments or surface plasmon resonance studies that help characterize how receptors and ligands interact. Since the protein is tag-free, binding studies likely reflect what actually happens with native protein-receptor interactions - no worrying about fusion tags interfering with the process. These studies may help reveal species-specific differences in IL-13 receptor binding when compared to human IL-13.
3. Antibody Development and Validation
This recombinant protein works as an antigen for generating mouse IL-13-specific antibodies or for validating antibodies that already exist in research applications. The high purity and well-defined sequence (M+22-131aa) make it nearly ideal as a standard for testing antibody specificity and examining cross-reactivity. Researchers often reach for this protein when developing ELISAs, validating Western blots, or setting up immunoprecipitation experiments. The confirmed biological activity suggests that antibodies developed against this protein should recognize the functionally relevant form of IL-13.
4. Protein-Protein Interaction Studies
The recombinant mouse IL-13 proves useful in pull-down assays and co-immunoprecipitation experiments designed to identify and characterize protein interactions within IL-13 signaling networks. Since its biological activity confirms proper protein folding, it appears well-suited for studying interactions with signaling intermediates and regulatory proteins. Scientists can examine how IL-13 forms complexes with its receptors and associated signaling molecules. The low endotoxin content helps ensure that any observed interactions are specific to IL-13 rather than artifacts from bacterial contamination.
5. Comparative Cytokine Function Studies
This mouse IL-13 protein opens up possibilities for comparative studies between mouse and human IL-13 functions in cross-species research models. Researchers can pair it with human IL-13 to investigate what may be species-specific differences in cytokine activity and receptor selectivity. The defined expression region and biological activity data provide a standardized tool for such comparative analyses. These studies might inform how findings from mouse models translate to human biology and help validate whether murine IL-13 research truly applies to human systems.
