Mouse Interleukin 17,IL-17 ELISA Kit

Mouse Interleukin 17,IL-17 ELISA Kit

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Il17a Proteins

Il17a Proteins

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ELISA Kit
Mouse Interleukin 17,IL-17 ELISA Kit

Specification
Target

Code

CSB-E04608m

See More Details

Product Type

ELISA Kit

Size

96T,5×96T,10×96T

Uniprot No.

Q62386

Lead Time

3-5 working days

Abbreviation

IL17A

Target Name

interleukin 17A

Alias

CTLA8, IL-17, IL-17A, IL17, cytotoxic T-lymphocyte-associated antigen 8|cytotoxic T-lymphocyte-associated protein 8|cytotoxic T-lymphocyte-associated serine esterase 8|interleukin 17 (cytotoxic T-ly

Species

Mus musculus (Mouse)

Sample Types

serum, plasma, tissue homogenates, cell culture supernates

Detection Range

47 pg/mL-3000 pg/mL

Sensitivity

11.75 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Protocol

Instructions

Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.

Assay Principle

quantitative

Measurement

Sandwich

Target Details

This protein is a proinflammatory cytokine produced by activated T cells. This cytokine regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of this cytokine are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis.

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-17 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	89-101
1:2	Average %	95
	Range %	90-100
1:4	Average %	88
	Range %	81-99
1:8	Average %	90
	Range %	81-98

Recovery

The recovery of mouse IL-17 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	88-100
EDTA plasma (n=4)	94	90-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
3000	2.807	2.909	2.858	2.690
1500	2.386	2.334	2.360	2.192
750	1.568	1.603	1.586	1.418
375	0.835	0.874	0.855	0.687
187.5	0.442	0.459	0.451	0.283
94	0.317	0.334	0.326	0.158
47	0.254	0.243	0.249	0.081
0	0.162	0.174	0.168

References

Mangiferin suppresses allergic asthma symptoms by decreased Th9 and Th17 responses and increased Treg response Yun C, et al,Molecular Immunology,2019
Anti-inflammatory Property of Galectin-1 in a Murine Model of Allergic Airway Inflammation Yunxiang Lv,Journal of Immunology Research,2019
Mesenchymal stromal cells-derived exosomes alleviate ischemia/reperfusion injury in mouse lung by transporting anti-apoptotic miR-21-5p Li JW, et al,European Journal Of Pharmacology,2019
Effects of melatonin on acute brain reperfusion stress: role of Hippo signaling pathway and MFN2-related mitochondrial protection Lan S, et al,Cell Stress & Chaperones,2019
The effects of resiquimod in an ovalbumin-induced allergic rhinitis model Qu Set al,Int Immunopharmacol.,2018
Imbalance of Th17/Treg in the Pathogenesis of Mice with Paraquat-induced Acute Lung Injury Xia Yang.et al,Iranian Journal of Allergy, Asthma and Immunology,2017
The Extracts of Morinda officinalis and Its Hairy Roots Attenuate Dextran Sodium Sulfate-Induced Chronic Ulcerative Colitis in Mice by Regulating Inflammation and Lymphocyte Apoptosis Jian Liang.et al,Front. Immunol.,2017
Comparison of the roles of house dust mite allergens, ovalbumin and lipopolysaccharides in the sensitization of mice to establish a model of severe neutrophilic asthma Aijun Jia.et al,Experimental and Therapeutic Medicine,2017
Isorhamnetin attenuates collagen-induced arthritis via modulating cytokines and oxidative stress in mice Wang X. et al,Int J Clin Exp Med,2015
High doses of caffeine reduce in vivo osteogenic activity in prepubertal rats Shin J. et al,J Anat.,2015
Serum sclerostin isDecreased following vitamin D treatment in young vitamin D-deficient female adults Cidem M. et al,Rheumatol Int. ,2015
IFN-r induced by IL-12 administration prevents diabetes by inhibiting pathogenic IL-17 production in NOD mice Jun Zhang et al,Journal of Autoimmunity,2012

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Function	Ligand for IL17RA
Subcellular Location	Secreted
Protein Families	IL-17 family
Tissue Specificity	Restricted to a subset of activated T-cells.
Database Links	KEGG: mmu:16171 STRING: 10090.ENSMUSP00000027061 UniGene: Mm.5419
Pathway	IL-17 signaling pathway Th17 cell differentiation

Il17a Protein

Il17a Proteins for Mus musculus (Mouse)

Code	Product Name	Source
CSB-AP004781MO	Recombinant Mouse Interleukin-17A(Il17a),partial (Active)	Mammalian cell
CSB-YP720298MO CSB-EP720298MO CSB-BP720298MO CSB-MP720298MO	Recombinant Mouse Interleukin-17A(Il17a)	Yeast E.coli Baculovirus Mammalian cell

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.