Mouse Interleukin 17,IL-17 ELISA Kit

Instructions
Code CSB-E04608m
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application

Product Details

Target Name interleukin 17A
Alternative Names CTLA8, IL-17, IL-17A, IL17, cytotoxic T-lymphocyte-associated antigen 8|cytotoxic T-lymphocyte-associated protein 8|cytotoxic T-lymphocyte-associated serine esterase 8|interleukin 17 (cytotoxic T-ly
Abbreviation IL17A
Uniprot No. Q62386
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates, cell culture supernates
Detection Range 47 pg/mL-3000 pg/mL
Sensitivity 11.75 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-17 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 95  
Range % 89-101  
1:2 Average % 95  
Range % 90-100  
1:4 Average % 88  
Range % 81-99  
1:8 Average % 90  
Range % 81-98  
Recovery
The recovery of mouse IL-17 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 93 88-100  
EDTA plasma (n=4) 94 90-99  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
3000 2.807 2.909 2.858 2.690  
1500 2.386 2.334 2.360 2.192  
750 1.568 1.603 1.586 1.418  
375 0.835 0.874 0.855 0.687  
187.5 0.442 0.459 0.451 0.283  
94 0.317 0.334 0.326 0.158  
47 0.254 0.243 0.249 0.081  
0 0.162 0.174 0.168    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days
Still Have Questions? Leave a Message or Start an on-line Chat

Citations

Target Data

Function Ligand for IL17RA
Subcellular Location Secreted
Protein Families IL-17 family
Tissue Specificity Restricted to a subset of activated T-cells.
Database Links

KEGG: mmu:16171

STRING: 10090.ENSMUSP00000027061

UniGene: Mm.5419

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