Mouse Interleukin 17,IL-17 ELISA Kit

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Mouse Interleukin 17,IL-17 ELISA Kit

Name: Mouse Interleukin 17,IL-17 ELISA Kit
Brand: CUSABIO
SKU: CSB-E04608m

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Code	CSB-E04608m
Size	96T,5×96T,10×96T
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Target Data

Target Name

interleukin 17A

Alternative Names

CTLA8, IL-17, IL-17A, IL17, cytotoxic T-lymphocyte-associated antigen 8|cytotoxic T-lymphocyte-associated protein 8|cytotoxic T-lymphocyte-associated serine esterase 8|interleukin 17 (cytotoxic T-ly

Abbreviation

IL17A

Uniprot No.

Q62386

Species

Mus musculus (Mouse)

Sample Types

serum, plasma, tissue homogenates, cell culture supernates

Detection Range

47 pg/mL-3000 pg/mL

Sensitivity

11.75 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-17 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	89-101
1:2	Average %	95
	Range %	90-100
1:4	Average %	88
	Range %	81-99
1:8	Average %	90
	Range %	81-98

Recovery

The recovery of mouse IL-17 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	88-100
EDTA plasma (n=4)	94	90-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
3000	2.807	2.909	2.858	2.690
1500	2.386	2.334	2.360	2.192
750	1.568	1.603	1.586	1.418
375	0.835	0.874	0.855	0.687
187.5	0.442	0.459	0.451	0.283
94	0.317	0.334	0.326	0.158
47	0.254	0.243	0.249	0.081
0	0.162	0.174	0.168

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

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The effects of resiquimod in an ovalbumin-induced allergic rhinitis model Qu Set al,Int Immunopharmacol.,2018
Imbalance of Th17/Treg in the Pathogenesis of Mice with Paraquat-induced Acute Lung Injury Xia Yang.et al,Iranian Journal of Allergy, Asthma and Immunology,2017
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Isorhamnetin attenuates collagen-induced arthritis via modulating cytokines and oxidative stress in mice Wang X. et al,Int J Clin Exp Med,2015
High doses of caffeine reduce in vivo osteogenic activity in prepubertal rats Shin J. et al,J Anat.,2015
Serum sclerostin isDecreased following vitamin D treatment in young vitamin D-deficient female adults Cidem M. et al,Rheumatol Int. ,2015
IFN-r induced by IL-12 administration prevents diabetes by inhibiting pathogenic IL-17 production in NOD mice Jun Zhang et al,Journal of Autoimmunity,2012

Function	Ligand for IL17RA
Subcellular Location	Secreted
Protein Families	IL-17 family
Tissue Specificity	Restricted to a subset of activated T-cells.
Database Links	KEGG: mmu:16171 STRING: 10090.ENSMUSP00000027061 UniGene: Mm.5419

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

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