Mouse Interleukin 17,IL-17 ELISA Kit

Code CSB-E04608m
See More Details
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. Q62386
Lead Time 3-5 working days
Abbreviation IL17A
Target Name interleukin 17A
Alias CTLA8, IL-17, IL-17A, IL17, cytotoxic T-lymphocyte-associated antigen 8|cytotoxic T-lymphocyte-associated protein 8|cytotoxic T-lymphocyte-associated serine esterase 8|interleukin 17 (cytotoxic T-ly
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates, cell culture supernates
Detection Range 47 pg/mL-3000 pg/mL
Sensitivity 11.75 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This protein is a proinflammatory cytokine produced by activated T cells. This cytokine regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of this cytokine are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis.
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-17 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 95  
Range % 89-101  
1:2 Average % 95  
Range % 90-100  
1:4 Average % 88  
Range % 81-99  
1:8 Average % 90  
Range % 81-98  
Recovery
The recovery of mouse IL-17 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 93 88-100  
EDTA plasma (n=4) 94 90-99  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
3000 2.807 2.909 2.858 2.690  
1500 2.386 2.334 2.360 2.192  
750 1.568 1.603 1.586 1.418  
375 0.835 0.874 0.855 0.687  
187.5 0.442 0.459 0.451 0.283  
94 0.317 0.334 0.326 0.158  
47 0.254 0.243 0.249 0.081  
0 0.162 0.174 0.168    
References
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Function Ligand for IL17RA
Subcellular Location Secreted
Protein Families IL-17 family
Tissue Specificity Restricted to a subset of activated T-cells.
Database Links

KEGG: mmu:16171

STRING: 10090.ENSMUSP00000027061

UniGene: Mm.5419

Pathway IL-17 signaling pathway
Th17 cell differentiation

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