Mouse Interleukin 22(IL-22) ELISA Kit

Code CSB-E13513m
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. Q9JJY9
Lead Time 3-5 working days
Abbreviation IL22
Target Name interleukin 22
Alias IL-21, IL-22, IL-D110, IL-TIF, IL21, ILTIF, MGC79382, MGC79384, TIFIL-23, TIFa, zcyto18, IL-10-related T-cell-derived inducible factor|interleukin 21
Species Mus musculus (Mouse)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 0.312 ng/mL-20 ng/mL
Sensitivity 0.078 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-22 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %106
Range %101-113
1:2Average %97
Range %92-103
1:4Average %90
Range %85-94
1:8Average %94
Range %90-98
Recovery
The recovery of mouse IL-22 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9590-100
EDTA plasma (n=4)8984-93
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
202.309 2.438 2.374 2.157
101.628 1.729 1.679 1.462
51.193 1.221 1.207 0.990
2.50.824 0.805 0.815 0.598
1.250.605 0.627 0.616 0.399
0.6250.462 0.450 0.456 0.239
0.3120.327 0.312 0.320 0.103
00.220 0.214 0.217
References
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Function Cytokine that contributes to the inflammatory response in vivo.
Subcellular Location Secreted
Protein Families IL-10 family
Database Links

KEGG: mmu:50929

STRING: 10090.ENSMUSP00000094449

UniGene: Mm.103585

Pathway Jak-STAT signaling pathway
Th17 cell differentiation

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