Mouse Interleukin 5,IL-5 ELISA KIT

Instructions
Code CSB-E04637m
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name interleukin 5 (colony-stimulating factor, eosinophil)
Alternative Names Il5 ELISA Kit; Il-5Interleukin-5 ELISA Kit; IL-5 ELISA Kit; B-cell growth factor II ELISA Kit; BCGF-II ELISA Kit; Cytotoxic T-lymphocyte inducer ELISA Kit; Eosinophil differentiation factor ELISA Kit; T-cell replacing factor ELISA Kit; TRF ELISA Kit
Abbreviation IL5
Uniprot No. P04401
Species Mus musculus (Mouse)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 31.25 pg/mL-2000 pg/mL
Sensitivity 7.8 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 85  
Range % 80-90  
1:2 Average % 99  
Range % 93-105  
1:4 Average % 97  
Range % 92-102  
1:8 Average % 95  
Range % 91-101  
Recovery
The recovery of mouse IL-5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 92 86-99  
EDTA plasma (n=4) 95 90-100  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
2000 2.336 2.252 2.294 2.203  
1000 1.699 1.686 1.693 1.602  
500 1.247 1.148 1.198 1.107  
250 0.913 0.877 0.895 0.804  
125 0.571 0.525 0.548 0.457  
62.5 0.315 0.294 0.305 0.214  
31.25 0.198 0.184 0.191 0.100  
0 0.092 0.090 0.091    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Citations

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Target Data

Function Factor that induces terminal differentiation of late-developing B-cells to immunoglobulin secreting cells.
Gene References into Functions
  1. binding of IL-5 to IL-5Ralpha receptors enhances angiogenic responses by stimulating the expression of HSP70-1 via the eNOS signaling pathway. PMID: 28317868
  2. IL-33 acts directly on bone marrow ILC2s, making them an early source of IL-5 and part of a process that is central in IL-33-driven eosinophilia. PMID: 28921511
  3. Obesity alters the lung neutrophil infiltration to enhance breast cancer metastasis through IL5 and GM-CSF. PMID: 28737771
  4. these studies establish a basal defect in eosinophilopoiesis in IL-33- and ST2-deficient mice and a mechanism whereby IL-33 supports eosinophils by driving both systemic IL-5 production and the expansion of IL-5Ralpha-expressing precursor cells PMID: 27683753
  5. Increased production of IL-5 from Peyer's patch cells and the restored Th1-type immune response might cause the production of abnormal IgA and might induce the glomerular deposition of IgA in IGA nephropathy. PMID: 26719095
  6. selective proliferation of IgM rheumatoid factor-secreting B-1a cells is induced by co-stimulation by the specific pathogen antigen and IL-5 in the development of MC in Capillaria hepatica-infected mice PMID: 25452118
  7. IL5, a cytokine involved in allergic and infectious diseases, facilitates metastatic colonization through recruitment of sentinel eosinophils and regulation of other inflammatory/immune cells in the microenvironment of the distal lung. PMID: 25691457
  8. Data (including data from knockout mice) suggest that up-regulation of IL5 production in lungs during influenza virus infection is due to infiltration of natural killer cells and alveolar macrophages into infected lung tissue. PMID: 24068930
  9. A decrease in the levels of IL-5, IL-9, and IL-6R in the BALF. PMID: 24246030
  10. eosinophils express CAR4 following IL-5 or allergen exposure, and that CAR4 is involved in regulating the lung transcriptome associated with allergic airway inflammation PMID: 24808371
  11. Protection of montelukast on OVA-induced eosinophilic gastroenteritis via modulating IL-5, eotaxin-1 and MBP expression. PMID: 23855447
  12. Together, these studies support the conclusion that surfactant protein D increases susceptibility to Cryptococcus neoformans infection by promoting Cryptococcus neoformans-driven pulmonary IL-5 and eosinophil infiltration. PMID: 24478083
  13. IL5 induced eosinophils and cysteinyl leukotrienes are involved in the pathology of mite antigen-induced chronic asthma model. PMID: 23942524
  14. Id3 is a key regulator of natural helper cell IL-5 production and B-1a B cell homeostasis. PMID: 24115031
  15. Interleukin-5 plays a key role in mouse strain- dependent susceptibility to contact hypersensitivity through its effects on initiator B cells. PMID: 23711860
  16. macrophage IL-5 is a target gene for LXR activation, and the induction of macrophage IL-5 expression can be related to LXR-inhibited atherosclerosis. PMID: 23150660
  17. Ang II induces increased Th2 cytokines IL-5 and IL-10 early in the course of experimental abdominal aortic aneurysm formation, and inhibition of IL-5 prevents AAA formation suggesting an important role. PMID: 22459292
  18. Sex difference in IL-5 production by splenocytes might be due, at least in part, to the sex difference in the sensitivity of CD4+ T cells to suppression by CD8+ T cells. PMID: 22627364
  19. Invading tumor cells enhance and increase local IL-5 production from innate IL-5-producing non-T lymphoid cells residing in the intestine, peritoneal cavity and lungs of naive mice. PMID: 22174445
  20. PARP-1 regulates Il-5 production through calpain degradation of STAT-6 in a murine asthma model. PMID: 21276008
  21. IL-5 production by splenocytes triggered by TCR activation was higher in female mice than in male mice, and the difference might be attributable to sex differences in CD4+ and CD8+ T cell functions. PMID: 21646791
  22. Transnasal administration of liposome- mediated IL12 could depress the expression of IL-5 in bone marrow, peripheral blood, and nasal mucosa in allergic rhinitis. PMID: 19954023
  23. Exacerbation of oxazolone colitis by infection with the helminth Hymenolepis diminuta: involvement of IL-5 and eosinophils. PMID: 21037078
  24. Greater antigen-induced Th2 IL-5 production by bronchial lymph node cells from female mice was associated with enhanced Th2 cell differentiation and increased expression of the Th2-specific transcription factor, GATA-3. PMID: 20337994
  25. IL-5 production by bronchial epithelial cells can impact the microenvironment of the lung, modifying pathologic and protective immune responses in the airways PMID: 20494340
  26. IL-5 promotes eosinophil trafficking to the esophagus PMID: 11859139
  27. IL-5 is required for the development of tissue and marrow eosinophilia, the formation of eosinophil/basophil colony-forming units, and the early development of symptoms in experimental allergic rhinitis. PMID: 11884474
  28. Role of IL-5 during primary and secondary immune response to acetylcholine receptor. PMID: 11960640
  29. mechanism of synergism between eotaxin and IL5, facilitating the selective recruitment of eosinophils into sites of allergic inflammation PMID: 12083417
  30. A putative Bcl6-binding DNA sequence which acts as a silencer element has been identified in the 3' untranslated region of IL-5 cDNA. PMID: 12097386
  31. IL-5 alone does not account for the complexities of bronchopulmonary hyperreactivity or of eosinophil tissue trapping PMID: 12231478
  32. effects of constitutive interleukin-5 (IL-5) expression and overabundance of eosinophils on the development and function of the mammary gland, uterus, and ovary PMID: 12620930
  33. IL-5 appears to be required for the accumulation of eosinophils and airway hyperresponsiveness in the inflammatory lung. PMID: 12660425
  34. The ability of IL-13 to induce eosinophilic esophagitis was abolished in STAT6-deficient mice, nearly completely ablated in IL-5-deficient mice, and significantly diminished in eotaxin-1-deficient mice. PMID: 14598258
  35. immune effector mechanisms in murine filarial infection are dependent on both IFN-gamma and IL-5, whose synergistic effects may be mediated, at least in part, by neutrophils for the control of adult worms. PMID: 14638787
  36. These results suggest an important role for interleukin-5, eosinophils, alphaVbeta6 integrin, and TGF-beta in airway remodeling. PMID: 14966564
  37. Pulmonary fibrosis lesions are abolished in sensitized and allergen-exposed IL-5 receptor-null mice, whereas they are markedly accentuated in IL-5 transgenic animals. PMID: 14975941
  38. Marked impairment of the maintenance of mature B-1 lymphocyte survival and homeostatic proliferation is demonstrated by blocking IL-5 signals. The key question is to what extent IL-5 is involved in mature B-1 cell survival and homeostatic proliferation. PMID: 15128785
  39. IL-5 participates in the pathogenesis of ileitis in SAMP1/Yit mice. PMID: 15162425
  40. CD4(-)c-kit(-)CD3epsilon(-)IL-2Ralpha(+) Peyer's patch cells are capable of secreting a high level of IL-5 in response to IL-2 PMID: 15214040
  41. IL-5 links adaptive and natural immunity for epitopes of oxidized LHDL and protects from arteriosclerosis. PMID: 15286809
  42. Anti-IL-5 was able to reduce eosinophil numbers in all tissue compartments, as well as BrdU+ eosinophils and CD34+ progenitor cells, and in all instances to a greater extent than anti-IL-9. PMID: 15823208
  43. the increase in airway eosinophilia seen with COX inhibition is dependent on IL-5, whereas the increase in airway hyperresponsiveness is not PMID: 16339565
  44. IL-5 is not necessary for differential splicing to occur in vivo, as all three forms of the IL-5R alpha are detected in both strains of IL-5 gene-deleted mice PMID: 16856933
  45. It was found that MCA-induced tumor incidence and growth were significantly attenuated in IL-5 transgenic mice. PMID: 17371978
  46. Il-5 level peaked at 7 dpi in bronchoalveolar lavage fluid. PMID: 17487773
  47. IL-5 gene delivery suppresses sensitization to antigen (ovalbumin) by upregulating transforming growth factor beta 1-dependent signaling to CD4-expressing T cells, thus suppressing allergic airway inflammation. PMID: 17579048
  48. cyclic AMP signals enhance histone H3 acetylation at the IL-5 promoter and the concerted binding of GATA-3 and NFATc to the promoter. PMID: 18772129
  49. there is a reciprocal relationship between inducible nitric oxide synthase and poly(ADP-ribose) polymerase-1; expression of inducible nitric oxide synthase may be dispensable for eosinophilia after interleukin-5 production PMID: 18829681
  50. Healing was significantly delayed in IL-5-overexpressing mice with wounds gaping wider and exhibiting impaired re-epithelialization PMID: 18839016

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Subcellular Location Secreted
Protein Families IL-5 family
Database Links

KEGG: mmu:16191

STRING: 10090.ENSMUSP00000043369

UniGene: Mm.4461

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