Mouse Macrophage-Derived Chemokine,MDC ELISA kit

Code CSB-E04661m
Size 96T,5×96T,10×96T
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Product Details

Target Name
chemokine (C-C motif) ligand 22
Alternative Names
Ccl22 ELISA Kit; Abcd1 ELISA Kit; Scya22C-C motif chemokine 22 ELISA Kit; Activated B and dendritic cell-derived ELISA Kit; CC chemokine ABCD-1 ELISA Kit; Small-inducible cytokine A22 ELISA Kit
Abbreviation
Uniprot No.
Species
Mus musculus (Mouse)
Sample Types
serum, plasma, tissue homogenates, cell culture supernates, cell lysates
Detection Range
31.25 pg/mL-2000 pg/mL
Sensitivity
23.672 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse MDC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 89
Range % 83-92
1:2 Average % 100
Range % 92-103
1:4 Average % 94
Range % 81-98
1:8 Average % 99
Range % 90-103
Recovery
The recovery of mouse MDC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 99 89-103
EDTA plasma (n=4) 93 84-98
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
2000 2.888 2.812 2.850 2.683
1000 2.589 2.513 2.551 2.384
500 2.110 2.168 2.139 1.972
250 1.463 1.446 1.455 1.288
125 0.810 0.827 0.819 0.652
62.5 0.537 0.554 0.546 0.379
31.25 0.320 0.311 0.316 0.149
0 0.169 0.165 0.167  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Mouse CCL22 ELISA Kit was designed for the quantitative measurement of Mouse CCL22 protein in serum, plasma, tissue homogenates, cell culture supernates, cell lysates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 23.672 pg/mL.

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Target Background

Function
(From Uniprot)
Chemotactic for activated T-lymphocytes. May play an important role in the collaboration of dendritic cells and B-lymphocytes with T-cells in immune responses.
Gene References into Functions
  1. the subset of peritoneal CD11b(+)CD169(+) macrophages increased and CCL22 expression level decreased significantly during the DSS-induced colitis PMID: 28466432
  2. Intratumoral administration of anti-CCL22 antibody inhibited B16F10 melanoma growth. PMID: 28668835
  3. this study shows that intravenous injection of apoptotic cells induces a subsequent increase in CCL22 expression and CCR4+ Treg cells, which contribute to the maintenance of immune homeostasis at least partially by splenic CD8alpha+ CD103+ dendritic cells PMID: 26868141
  4. CCL22-specific antibodies reveal that engagement of two distinct binding domains on CCL22 is required for CCR4-mediated function. PMID: 26683175
  5. CCL22 was localised mainly on the cell surface and or in the cytoplasm. Within sections of omental milky spot micrometastases, CCR4 was recognised on or in gastric cancer cells, constituent cells milky spots, blood cells and blood endothelial cells PMID: 25245466
  6. results show that the IL-4/CCL22/CCR4 axis is involved in the migration of Tregs to osteolytic lesion sites, and attenuates development of lesions by inhibiting inflammatory migration and the production of proinflammatory and osteoclastogenic mediators PMID: 25264308
  7. CCL22 has a role in Treg skin homing to suppress depigmentation PMID: 25634358
  8. CCL22 is a novel mediator of lung inflammation following hemorrhage and resuscitation PMID: 25136780
  9. The immunomodulatory properties of CCL22 could be harnessed for prevention of graft rejection and type 1 diabetes as well as other autoimmune disorders. PMID: 25740943
  10. Data indicate macrophage-derived chemokine CCL22 as an adjuvant could enhance the immune protective effect of NTHi-P6 protein vaccine to an extent. PMID: 25200152
  11. islet expression of CCL22 recruits Tregs and attenuates autoimmune destruction of beta cell PMID: 21737880
  12. Data show that the presence of the Ccr4 and Ccl22 transcripts were detected in brain slices. PMID: 21177120
  13. These results suggest that CCL22 functions to regulate development of experimental autoimmune encephalomyelitis through macrophage chemoattraction and effector function. PMID: 20940325
  14. role of CCL22 for the recruitment of eosinophils during allergic pleurisy PMID: 12629149
  15. functionally increased expression during Salmonella enterica serovar Typhimurium infection of dendritic cells PMID: 15731072
  16. Regulatory elements of the CCL22 gene required to mediate a strong and highly specific expression are included in a 4.1-kb promoter region, with the major elements active in dendritic cells and B cells being located in a small proximal 250-bp region. PMID: 15843561
  17. CCL22 was selectively upregulated in osteoclast-like cells derived from RAW264.7 cells and mouse bone marrow cells upon stimulation with RANKL (receptor activator of nuclear factor-kappaB ligand). PMID: 16821125
  18. IgE appears to be capable of stimulating basophils to produce MDC in the absence of a specific Ag, which may contribute to IgE-mediated and/or Th2-predominant allergic inflammation. PMID: 18832724
  19. When isolated, only natural killer (NK)-containing cellular fractions secrete CCL22, and the same fraction isolated from metastatic Lewis lung carcinoma (LLC)-bearing lungs secrete higher levels. PMID: 19234170
  20. Mice genetically deficient in CCR4 (the receptor for CCL22) show markedly reduced indoleamine dioxygenase (IDO) expression in MLN-DCs and support the involvement of the CCL22/CCR4 axis in IDO induction. PMID: 19843945

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Subcellular Location
Secreted.
Protein Families
Intercrine beta (chemokine CC) family
Tissue Specificity
Expressed by activated splenic B-lymphocytes and dendritic cells. Low expression in lung, thymocytes, lymph node, and unstimulated splenic cells.
Database Links
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