Product Details

Purity

>97% as determined by SDS-PAGE.

Endotoxin

Less than 1.0 EU/μg as determined by LAL method.

Activity

Not Test

Target Names

Ccl22

Uniprot No.

O88430

Research Area

Immunology

Alternative Names

Ccl22; Abcd1; Scya22C-C motif chemokine 22; Activated B and dendritic cell-derived; CC chemokine ABCD-1; Small-inducible cytokine A22

Species

Mus musculus (Mouse)

Source

E.Coli

Expression Region

25-92aa

Complete Sequence

GPYGANVEDS ICCQDYIRHP LPSRLVKEFF WTSKSCRKPG VVLITVKNRD ICADPRQVWV KKLLHKLS

Mol. Weight

7.8 kDa

Protein Length

Full Length of Mature Protein

Tag Info

Tag-Free

Form

Liquid or Lyophilized powder

Buffer

0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl, lyophilized

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

5-10 business days

Datasheet & COA

Please contact us to get it.

Description

The recombinant Mouse Ccl22,Abcd1,Scya22 was expressed with the amino acid range of 25-92. The theoretical molecular weight of the Ccl22,Abcd1,Scya22 protein is 7.8 kDa. The Ccl22,Abcd1,Scya22 protein was expressed in e.coli. The Ccl22,Abcd1,Scya22 coding gene included the Tag-Free, which simplifies the detection and purification processes of the recombinant Ccl22,Abcd1,Scya22 protein in following stages of expression and purification.

Mouse C-C motif chemokine 22 (Ccl22) is a small cytokine belonging to the CC chemokine family. It plays a key role in immune regulation and inflammation. Ccl22 is primarily secreted by dendritic cells, macrophages, and certain other immune cells. Its main function is to attract and modulate the activity of immune cells, particularly T lymphocytes, by binding to its receptor, CCR4. Research areas involving Ccl22 focus on understanding its role in immune responses, inflammation, and various diseases, including autoimmune disorders and cancer. Investigating the molecular mechanisms by which Ccl22 influences immune cell migration and function contributes to the development of potential therapeutic strategies targeting the chemokine system for immunomodulation and disease intervention.

Target Background

Function

Chemotactic for activated T-lymphocytes. May play an important role in the collaboration of dendritic cells and B-lymphocytes with T-cells in immune responses.

Gene References into Functions

the subset of peritoneal CD11b(+)CD169(+) macrophages increased and CCL22 expression level decreased significantly during the DSS-induced colitis PMID: 28466432
Intratumoral administration of anti-CCL22 antibody inhibited B16F10 melanoma growth. PMID: 28668835
this study shows that intravenous injection of apoptotic cells induces a subsequent increase in CCL22 expression and CCR4+ Treg cells, which contribute to the maintenance of immune homeostasis at least partially by splenic CD8alpha+ CD103+ dendritic cells PMID: 26868141
CCL22-specific antibodies reveal that engagement of two distinct binding domains on CCL22 is required for CCR4-mediated function. PMID: 26683175
CCL22 was localised mainly on the cell surface and or in the cytoplasm. Within sections of omental milky spot micrometastases, CCR4 was recognised on or in gastric cancer cells, constituent cells milky spots, blood cells and blood endothelial cells PMID: 25245466
results show that the IL-4/CCL22/CCR4 axis is involved in the migration of Tregs to osteolytic lesion sites, and attenuates development of lesions by inhibiting inflammatory migration and the production of proinflammatory and osteoclastogenic mediators PMID: 25264308
CCL22 has a role in Treg skin homing to suppress depigmentation PMID: 25634358
CCL22 is a novel mediator of lung inflammation following hemorrhage and resuscitation PMID: 25136780
The immunomodulatory properties of CCL22 could be harnessed for prevention of graft rejection and type 1 diabetes as well as other autoimmune disorders. PMID: 25740943
Data indicate macrophage-derived chemokine CCL22 as an adjuvant could enhance the immune protective effect of NTHi-P6 protein vaccine to an extent. PMID: 25200152
islet expression of CCL22 recruits Tregs and attenuates autoimmune destruction of beta cell PMID: 21737880
Data show that the presence of the Ccr4 and Ccl22 transcripts were detected in brain slices. PMID: 21177120
These results suggest that CCL22 functions to regulate development of experimental autoimmune encephalomyelitis through macrophage chemoattraction and effector function. PMID: 20940325
role of CCL22 for the recruitment of eosinophils during allergic pleurisy PMID: 12629149
functionally increased expression during Salmonella enterica serovar Typhimurium infection of dendritic cells PMID: 15731072
Regulatory elements of the CCL22 gene required to mediate a strong and highly specific expression are included in a 4.1-kb promoter region, with the major elements active in dendritic cells and B cells being located in a small proximal 250-bp region. PMID: 15843561
CCL22 was selectively upregulated in osteoclast-like cells derived from RAW264.7 cells and mouse bone marrow cells upon stimulation with RANKL (receptor activator of nuclear factor-kappaB ligand). PMID: 16821125
IgE appears to be capable of stimulating basophils to produce MDC in the absence of a specific Ag, which may contribute to IgE-mediated and/or Th2-predominant allergic inflammation. PMID: 18832724
When isolated, only natural killer (NK)-containing cellular fractions secrete CCL22, and the same fraction isolated from metastatic Lewis lung carcinoma (LLC)-bearing lungs secrete higher levels. PMID: 19234170
Mice genetically deficient in CCR4 (the receptor for CCL22) show markedly reduced indoleamine dioxygenase (IDO) expression in MLN-DCs and support the involvement of the CCL22/CCR4 axis in IDO induction. PMID: 19843945