Mouse Proto-oncogene Wnt-1(WNT1) ELISA kit

Code CSB-EL026128MO
Size 96T,5×96T,10×96T
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Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
wingless-type MMTV integration site family, member 1
Alternative Names
Wnt1 ELISA kit; Int-1 ELISA kit; Wnt-1Proto-oncogene Wnt-1 ELISA kit; Proto-oncogene Int-1 ELISA kit
Abbreviation
WNT1
Uniprot No.
Species
Mus musculus (Mouse)
Sample Types
serum, plasma, tissue homogenates
Detection Range
18.75 pg/mL-1200 pg/mL
Sensitivity
4.68 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Stem Cells
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse WNT1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %90
Range %86-94
1:2Average %84
Range %80-88
1:4Average %89
Range %84-93
1:8Average %98
Range %94-102
Recovery
The recovery of mouse WNT1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 106100-110
EDTA plasma (n=4)9490-98
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
12002.749 2.627 2.688 2.543
6002.234 2.306 2.270 2.125
3001.493 1.534 1.514 1.369
1500.729 0.755 0.742 0.597
750.401 0.427 0.414 0.269
37.50.277 0.259 0.268 0.123
18.750.236 0.247 0.242 0.097
00.147 0.142 0.145
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Mouse WNT1 ELISA Kit was designed for the quantitative measurement of Mouse WNT1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 18.75 pg/mL-1200 pg/mL and the sensitivity is 4.68 pg/mL.

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 Q&A
Q:

a. The active ingredient of the stop solution and its concentration ?
b. The type, concentration, and state (liquid/lyophilized) of the preservatives used. Please name the components with preservatives ?
c. If the components are lyophilized, what is the concentration prior to reconstitution (i.e. mass of preservative used/ total mass of lyophilized vial contents)?
d. Expression host of the standards ?
e. Which components have H202 ( including other forms of peroxide such as urea hydrogen peroxide) ?Please list with the concentration of H202/peroxide in each, if any?

A:
Thanks for your inquiry!
CSB-EL026128MO
Stop solution is 4N sulfuric acid.
Preservatives is liquid proclin 300(1:3000);
Standard is 293 cell-derived recombinant protein. It is lyophilized powder.
The concentration after reconstitution is 1200pg/mL(pls refer to Page 9 in the manual);
The kit doesn't contain H2O2.
Pls let me know if you have any further questions. Thank you.
Q:

Just to clarify, Preservatives (proclin 300) is included only in the lyophilized standard ?
Also, please confirm that the only preservative is proclin 300? If not, please let me know the names and concentrations

A:
All components contain preservatives (proclin 300) except wash buffer、TMB Substrate and Stop Solution.

Target Background

Function
(From Uniprot)
Ligand for members of the frizzled family of seven transmembrane receptors. Acts in the canonical Wnt signaling pathway by promoting beta-catenin-dependent transcriptional activation. In some developmental processes, is also a ligand for the coreceptor RYK, thus triggering Wnt signaling. Plays an essential role in the development of the embryonic brain and central nervous system (CNS). Has a role in osteoblast function, bone development and bone homeostasis.
Gene References into Functions
  1. aberrant expression of AF1q may activate Wnt/beta-catenin signaling and result in podocyte injury. PMID: 29069662
  2. Wnt1a maintains characteristics of dermal papilla cells that induce mouse hair regeneration in a 3D preculture system PMID: 26118627
  3. These data suggested that Wnt/beta-catenin pathway might be a potential target to treat the LPS-induced inflammation in ALI. PMID: 29246763
  4. Wnt signaling regulates airway epithelial stem cells in adult murine submucosal glands. PMID: 27341073
  5. Pax9-dependent Wnt signaling has a role in palatogenesis and cleft palates PMID: 28893947
  6. Data show that autocrine Wnt secretion is important for the survival, chromosomal stability, differentiation, and tumorigenic potential of embryonic stem cells (ESCs). PMID: 28074006
  7. Results demonstrated functional differences in the molecular mechanisms downstream of Wnt1 function in the diencephalon, in relation to the spinal cord. Wnt1 signal determines the patterning of the diencephalic dorso-ventral axis PMID: 26452989
  8. Data show that both transgenic Wnt1-cre and P0-cre are similarly effective in deleting beta-catenin in the neural crest. PMID: 27184910
  9. data suggest that WNT1-related osteogenesis imperfecta and osteoporosis are caused in part by decreased mTORC1-dependent osteoblast function resulting from loss of WNT1 signaling in osteocytes. PMID: 28628032
  10. Administration of EET alters Wnt1, NOV, and HO-1 signaling to prevent obesity-induced cardiomyopathy in obese mice. PMID: 28576832
  11. Data indicate that Wnt1 proto-oncogene protein (WNT1) is the direct target of microRNA miR-34a in dendritic cell (DC). PMID: 28199987
  12. The data obtained from the 14-3-3epsilon/14-3-3zeta/Wnt1-Cre mice strongly indicate the importance of 14-3-3 proteins in the development of melanocyte lineages. PMID: 27001213
  13. High WNT1 promoted stem-ness characteristics and metastasis potential in sphere-forming liver cancer stem cells. PMID: 26735577
  14. Decreased expressions of Wnt1 and Wnt5a. PMID: 26537990
  15. we provide unequivocal evidence that epithelial-derived Wnt1 is sufficient to drive interstitial fibrosis in the absence of any injury. PMID: 26204899
  16. Data show that elongator protein 3 (Elp3) trigger Wnt-driven tumor initiation in the intestine, and Elp3 deficiency strongly impaired radiation-induced intestinal regeneration. PMID: 26527802
  17. Fasudil prevents loss of dopamine neurons in the MPTP mice model of PD through inflammatory inhibition via activation of PI3K/p-Akt and WNT1/Fzd1/beta-catenin cell signaling pathways. PMID: 26045742
  18. Wnt has a role in striatal synaptic degeneration and impaired motor behavior in adult mice PMID: 25318560
  19. Agonists of epoxyeicosatrienoic acids reduce infarct size and ameliorate cardiac dysfunction via activation of HO-1 and Wnt1 canonical pathway. PMID: 25677507
  20. this study gives new insights into transcriptional regulating mechanisms of Wnt-mediated Isl1 expression during cardiomyocyte differentiation. PMID: 24610003
  21. Data show that bone marrow mesenchymal stem cell (BM-MSC)-generated Wnt1a promotes the dermal papilla's ability to induce hair cycling and regeneration. PMID: 24961246
  22. modulates neuronal function, hippocampal neurogenesis, and synaptic plasticity and that downregulation of Wnt signaling could be involved in the cognitive decline associated with aging and also with the physiopathology of Alzheimer's disease (AD). PMID: 25443287
  23. BECN1 suppresses mammary tumorigenesis driven by WNT1 activation and following parity PMID: 25483966
  24. in the absence of retinoid signaling via RARbeta, reduced IGF-1 signaling results in suppression of epithelial-mesenchymal transition and delays tumorigenesis induced by the Wnt1 oncogene PMID: 25422594
  25. results revealed that in Wnt-driven tumors, an attenuation of IGF1R signaling accelerates tumorigenesis and promotes more aggressive phenotypes with potential implications for understanding TNBC pathobiology and treatment PMID: 25092896
  26. The swaying mouse is a model of osteogenesis imperfecta caused by WNT1 mutations. PMID: 24634143
  27. Results indicate an important role for the canonical Wnt/beta-catenin pathway in the dendritic cells (DCs)-mediated regulation of allergic responses in the lung. PMID: 24929002
  28. a broader role for Wnt/beta-catenin signaling in contributing to progenitor cell proliferation in nonhairy epithelia and interfollicular epidermis under homeostasis, is reported. PMID: 24315444
  29. Wnt activation, rather than mature hair follicles, is required for adipocyte generation in murine epidermis. PMID: 24706781
  30. Concomitant expression of Wnt1 and KRASG12D in pancreatic epithelium stimulates development of mucinous cystic neoplasm (MCN)-like lesions in female mice. PMID: 24067880
  31. The results indicate the involvement of Wnt1 and Fzd1 in the pathogenesis and development of amyotrophic lateral sclerosis. PMID: 23553522
  32. Marked elevation of Wnt1 expression in the ventral midbrain is correlated with disruption of the differentiation program of ventral dopaminergic neurons. PMID: 23648512
  33. WNT signaling, which is important in developmental processes of the nervous system, plays a critical role in neuropathic pain after sciatic nerve injury and bone cancer in rodents PMID: 23585476
  34. Lgr4 is critically involved in the maintenance of intestinal homeostasis and protection against inflammatory bowel disease through modulation of the Wnt/beta-catenin signaling pathway PMID: 23393138
  35. Axin2-positive tympanic border cells are Wnt responsive and can act as precursors to sensory epithelial cells in the postnatal cochlea. PMID: 23444352
  36. The spatial and temporal function of Wnt1 in midbrain and cerebellum patterning and in midbrain dopamine neuron development in vivo. PMID: 23444360
  37. Wnt1 and Wnt5a interact genetically and cooperate to promote midbrain DA neuron development in vivo. PMID: 23324743
  38. Matrix rigidity activates Wnt signaling through down-regulation of Dickkopf-1 protein PMID: 23152495
  39. cyclin-dependent kinase 2-associated protein 1 (CDK2AP1), an essential gene for early embryonic development, plays a role in pluripotency of ESC by engaging MBD3 to the promoter region of Wnt signaling genes PMID: 23074223
  40. Wnt1cre/Dicer mice fail to develop neural crest-derived cartilages, therefore, have no middle ear ossicles. PMID: 22778034
  41. Low-dose radiation stimulates neural stem cell proliferation, neurogenesis in the hippocampus, and animal learning, by triggering Wnt/beta-catenin signaling cascades. PMID: 22272614
  42. these results suggest that Wnt1 could enhance tumorigenesis by conferring stem cell properties on breast cancer cells PMID: 22846569
  43. Wnt1 is anti-lymphangiogenic by suppressing melanoma-derived VEGF-C expression. PMID: 22572818
  44. The balancing act between cell proliferation and mucus production to restore barrier integrity seems to depend upon the interplay between the Wnt/beta-catenin and Notch pathways in the transmissible murine colonic hyperplasia model. PMID: 22710872
  45. Dickkopf2 is a Wnt antagonist involved in regulation of glucose metabolism PMID: 22733757
  46. GSK3beta plays an essential role in Ras degradation and inhibition of this degradation pathway by aberrant Wnt/beta-catenin signaling may contribute to Ras-induced transformation in colorectal tumorigenesis PMID: 22494971
  47. Wnt1 expression is related to molecular identity and cell migration in cerebellum development in mice. PMID: 22173107
  48. dermal Wnt signaling/beta-catenin has roles in fibroblast proliferation and in the epidermal hair follicle initiation program PMID: 22434869
  49. lipid modification of Wnt1 is required for Wnt secretion, but not stability PMID: 22046319
  50. Acute ischaemic cardiac injury upregulates Wnt1 in the epicardium & later by fibroblasts in the region of injury. Wnt1 induces them to proliferate & express pro-fibrotic genes. PMID: 22085926

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Subcellular Location
Secreted, extracellular space, extracellular matrix. Secreted.
Protein Families
Wnt family
Tissue Specificity
Testis and mid-gestational embryos. In the testis, detected only in postmeiotic germ cells undergoing differentiation from round spermatids into mature spermatozoa. In the embryos, expression is restricted to the developing CNS in regions of the neural tu
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