Mouse Proto-oncogene Wnt-1(WNT1) ELISA kit

Code CSB-EL026128MO
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P04426
Lead Time 5-7 working days
Abbreviation WNT1
Protein Biological Process 1 Signaling Pathway
Target Name wingless-type MMTV integration site family, member 1
Alias INT1, Wingless-type MMTV integration site family, member 1 (oncogene INT1)
Species Mus musculus (Mouse)
Protein Biological Process 3 Wnt signaling pathway
Sample Types serum, plasma, tissue homogenates
Detection Range 18.75 pg/mL-1200 pg/mL
Sensitivity 4.68 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Stem Cells
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It is very conserved in evolution, and This protein is known to be 98% identical to the mouse Wnt1 protein at the amino acid level. The studies in mouse indicate that the Wnt1 protein functions in the induction of the mesencephalon and cerebellum. This gene was originally considered as a candidate gene for Joubert syndrome, an autosomal recessive disorder with cerebellar hypoplasia as a leading feature. However, further studies suggested that the gene mutations might not have a significant role in Joubert syndrome. This gene is clustered with another family member, WNT10B, in the chromosome 12q13 region.
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse WNT1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %90
Range %86-94
1:2Average %84
Range %80-88
1:4Average %89
Range %84-93
1:8Average %98
Range %94-102
Recovery
The recovery of mouse WNT1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 106100-110
EDTA plasma (n=4)9490-98
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
12002.749 2.627 2.688 2.543
6002.234 2.306 2.270 2.125
3001.493 1.534 1.514 1.369
1500.729 0.755 0.742 0.597
750.401 0.427 0.414 0.269
37.50.277 0.259 0.268 0.123
18.750.236 0.247 0.242 0.097
00.147 0.142 0.145
References
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Function Ligand for members of the frizzled family of seven transmembrane receptors. Acts in the canonical Wnt signaling pathway by promoting beta-catenin-dependent transcriptional activation (By similarity). In some developmental processes, is also a ligand for the coreceptor RYK, thus triggering Wnt signaling. Probable developmental protein. May be a signaling molecule important in CNS development. Is likely to signal over only few cell diameters. Prominent role in the induction of the mesencephalon and cerebellum. May play a crucial role in the morphogenesis of the neural tube and/or the early stages of CNS development. Has a role in osteoblast function and bone development (By similarity).
Subcellular Location Secreted, extracellular space, extracellular matrix
Protein Families Wnt family
Tissue Specificity Testis and mid-gestational embryos. In the testis, detected only in postmeiotic germ cells undergoing differentiation from round spermatids into mature spermatozoa. In the embryos, expression is restricted to the developing CNS in regions of the neural tu
Database Links

KEGG: mmu:22408

STRING: 10090.ENSMUSP00000023734

UniGene: Mm.1123

Pathway Hippo signaling pathway
mTOR signaling pathway
Wnt signaling pathway
Signaling pathways regulating pluripotency of stem cells
Excitatory synapse pathway
Inhibitors of axonal regeneration

Q&A and Customer Reviews

Q&A
Q:

a. The active ingredient of the stop solution and its concentration ?
b. The type, concentration, and state (liquid/lyophilized) of the preservatives used. Please name the components with preservatives ?
c. If the components are lyophilized, what is the concentration prior to reconstitution (i.e. mass of preservative used/ total mass of lyophilized vial contents)?
d. Expression host of the standards ?
e. Which components have H202 ( including other forms of peroxide such as urea hydrogen peroxide) ?Please list with the concentration of H202/peroxide in each, if any?

A:
Thanks for your inquiry!
CSB-EL026128MO
Stop solution is 4N sulfuric acid.
Preservatives is liquid proclin 300(1:3000);
Standard is 293 cell-derived recombinant protein. It is lyophilized powder.
The concentration after reconstitution is 1200pg/mL(pls refer to Page 9 in the manual);
The kit doesn't contain H2O2.
Pls let me know if you have any further questions. Thank you.
Q:

Just to clarify, Preservatives (proclin 300) is included only in the lyophilized standard ?
Also, please confirm that the only preservative is proclin 300? If not, please let me know the names and concentrations

A:
All components contain preservatives (proclin 300) except wash buffer、TMB Substrate and Stop Solution.
Customer Reviews

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