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Your Good Partner in Biology Research
| Code | CSB-E04754m |
| Size | 96T,5×96T,10×96T |
| See More Details |  |
| Have Questions? | Leave a Message or Start an on-line Chat |
| Target Name | vascular cell adhesion molecule 1 |
| Alternative Names | Vcam1 ELISA Kit; Vcam-1 ELISA Kit; Vascular cell adhesion protein 1 ELISA Kit; V-CAM 1 ELISA Kit; VCAM-1 ELISA Kit; CD antigen CD106 ELISA Kit |
| Abbreviation | VCAM1 |
| Uniprot No. | P29533 |
| Species | Mus musculus (Mouse) |
| Sample Types | serum, plasma, cell culture supernates, tissue homogenates |
| Detection Range | 39.062 pg/mL-2500 pg/mL |
| Sensitivity | 29.649 pg/mL |
| Assay Time | 1-5h |
| Sample Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Cardiovascular |
| Assay Principle | quantitative |
| Measurement | Sandwich |
| Precision |
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess.
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| Linearity |
To assess the linearity of the assay, samples were spiked with high concentrations of mouse VCAM-1/CD106 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Typical Data |
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
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| Troubleshooting and FAQs |
ELISA kit FAQs |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Lead Time | 3-5 working days |
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| Function | Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with integrin alpha-4/beta-1 (ITGA4/ITGB1) on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/ITGA4/ITGB1 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation. |
| Subcellular Location | Isoform 1: Cell membrane, Single-pass type I membrane protein, SUBCELLULAR LOCATION: Isoform 2: Cell membrane, Lipid-anchor, GPI-anchor |
| Tissue Specificity | Expressed on inflamed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflamed tissue. Expressed in the bone marrow. |
| Database Links |
STRING: 10090.ENSMUSP00000029574 UniGene: Mm.440909 |
