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Mouse Vascular endothelial cell growth factor receptor 2,VEGFR-2/Flk-1 ELISA kit

Citations (1)
Code CSB-E04764m
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
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Product Details

Target Name
kinase insert domain receptor (a type III receptor tyrosine kinase)
Alternative Names
Kdr ELISA Kit; Flk-1 ELISA Kit; Flk1 ELISA Kit; Vascular endothelial growth factor receptor 2 ELISA Kit; VEGFR-2 ELISA Kit; EC 2.7.10.1 ELISA Kit; Fetal liver kinase 1 ELISA Kit; FLK-1 ELISA Kit; Kinase NYK ELISA Kit; Protein-tyrosine kinase receptor flk-1 ELISA Kit; CD antigen CD309 ELISA Kit
Abbreviation
VEGFR-2/Flk-1
Uniprot No.
P35918
Species
Mus musculus (Mouse)
Sample Types
serum, plasma
Detection Range
7.8 pg/mL-500 pg/mL
Sensitivity
1.95 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cancer
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%        
Three samples of known concentration were tested twenty times on one plate to assess.    
Inter-assay Precision (Precision between assays): CV%<10%        
Three samples of known concentration were tested in twenty assays to assess.      
               
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse VEGFR-2/Flk-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
　 Sample Serum(n=4)    
1:100 Average % 86    
Range % 82-91    
1:200 Average % 101    
Range % 98-105    
1:400 Average % 87    
Range % 84-91    
1:800 Average % 100    
Range % 95-105    
Recovery
The recovery of mouse VEGFR-2/Flk-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
 
Sample Type Average % Recovery Range    
Serum (n=5) 89 85-94    
EDTA plasma (n=4) 107 103-110    
               
               
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
 
pg/ml OD1 OD2 Average Corrected    
500 2.564 2.536 2.550 2.438    
250 1.513 1.531 1.522 1.410    
125 0.985 0.961 0.973 0.861    
62.5 0.551 0.560 0.556 0.444    
31.2 0.345 0.351 0.348 0.236    
15.6 0.242 0.251 0.247 0.135    
7.8 0.174 0.176 0.175 0.063    
0 0.113 0.110 0.112 　    
ELISA Data Analysis
ELISA Standard Curve & Curve Expert software
Troubleshooting
and FAQs
ELISA kit FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Mouse VEGFR-2/Flk-1 ELISA Kit was designed for the quantitative measurement of Mouse VEGFR-2/Flk-1 protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 7.8 pg/mL-500 pg/mL and the sensitivity is 1.95 pg/mL.

Citations

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Target Background

Function
(From Uniprot)
Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFA, VEGFC and VEGFD. Plays an essential role in the regulation of angiogenesis, vascular development, vascular permeability, and embryonic hematopoiesis. Promotes proliferation, survival, migration and differentiation of endothelial cells. Promotes reorganization of the actin cytoskeleton. Isoforms lacking a transmembrane domain, such as isoform 2, may function as decoy receptors for VEGFA, VEGFC and/or VEGFD. Isoform 2 plays an important role as a negative regulator of VEGFA- and VEGFC-mediated lymphangiogenesis by limiting the amount of free VEGFA and/or VEGFC and by preventing their binding to FLT4. Modulates FLT1 and FLT4 signaling by forming heterodimers. Binding of vascular growth factors to isoform 1 leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate and the activation of protein kinase C. Mediates activation of MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Mediates phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, reorganization of the actin cytoskeleton and activation of PTK2/FAK1. Required for VEGFA-mediated induction of NOS2 and NOS3, leading to the production of the signaling molecule nitric oxide (NO) by endothelial cells. Phosphorylates PLCG1. Promotes phosphorylation of FYN, NCK1, NOS3, PIK3R1, PTK2/FAK1 and SRC.
Subcellular Location
Cell junction. Endoplasmic reticulum. Cell membrane.; [Isoform 1]: Cell membrane; Single-pass type I membrane protein. Cytoplasm. Nucleus. Cytoplasmic vesicle. Early endosome.; [Isoform 2]: Secreted.
Protein Families
Protein kinase superfamily, Tyr protein kinase family, CSF-1/PDGF receptor subfamily
Tissue Specificity
Expressed in endothelial cells (at protein level). Detected in embryonic endothelial cells, as well as hematopoietic stem and progenitor cells. Detected in vascular endothelium. Expressed at high levels in adult heart, lung, kidney, brain and skeletal mus
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