Mouse hepcidin(Hepc) ELISA Kit

Code CSB-E14395m
Size 96T,5×96T,10×96T
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Product Details

Target Name
hepcidin antimicrobial peptide
Alternative Names
Hamp ELISA Kit; Hamp1 ELISA Kit; Hepc ELISA Kit; Hepc1 ELISA Kit; Hepcidin ELISA Kit
Abbreviation
HAMP
Uniprot No.
Species
Mus musculus (Mouse)
Sample Types
serum, plasma, tissue homogenates
Detection Range
0.78 ng/mL-50 ng/mL
Sensitivity
0.195 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cardiovascular
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse Hepc in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 87  
Range % 83-91  
1:2 Average % 90  
Range % 85-96  
1:4 Average % 94  
Range % 89-99  
1:8 Average % 97  
Range % 93-102  
Recovery
The recovery of mouse Hepc spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 93 90-96  
EDTA plasma (n=4) 99 94-103  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
50 2.443 2.568 2.506 2.328  
25 1.525 1.598 1.562 1.384  
12.5 0.999 0.975 0.987 0.809  
6.25 0.560 0.552 0.556 0.378  
3.12 0.413 0.403 0.408 0.230  
1.56 0.308 0.319 0.314 0.136  
0.78 0.249 0.253 0.251 0.073  
0 0.177 0.179 0.178    
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This mouse hepcidin (HAMP) ELISA kit employs the quantitative sandwich enzyme immunoassay technique to measure the levels of mouse hepcidin in multiple samples, including serum, plasma, or tissue homogenates. The enzyme-substrate chromogenic reaction is also used to amplify the signal and quantify the levels of the analyte through the intensity of the colored product. The color intensity positively correlates with the amount of hepcidin bound in the initial step.

Hepcidin is an endogenous antimicrobial peptide that is produced by the liver and mainly functions to negatively regulate iron. It controls plasma iron levels by regulating the intestinal absorption of dietary iron, as well as the release of iron from macrophages and the transfer of iron stored in the hepatocytes. Hepcidin induces ferroportin internalization and degradation in target cells, mostly macrophages and enterocytes, leading to iron sequestration in macrophages and declined iron absorption from the intestine. The expression of hepcidin is induced by elevated iron concentration and inflammation but inhibited by anemia and hypoxia. Dysregulation of hepcidin is associated with iron disorder-related diseases, such as anemias, hereditary hemochromatosis, and β-thalassemia.

Customer Reviews and Q&A

 Customer Reviews
Average Rating:
3.0 - 1 reviews

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Sample type: Serum

Sample species: Mouse

Sample dilution: No dilution

Review: I have used this kit to evaluate the serum hepcidin levels in diseased and normal mice.

By Anonymous

Target Background

Function
(From Uniprot)
Liver-produced hormone that constitutes the main circulating regulator of iron absorption and distribution across tissues. Acts by promoting endocytosis and degradation of ferroportin, leading to the retention of iron in iron-exporting cells and decreased flow of iron into plasma. Controls the major flows of iron into plasma: absorption of dietary iron in the intestine, recycling of iron by macrophages, which phagocytose old erythrocytes and other cells, and mobilization of stored iron from hepatocytes.
Gene References into Functions
  1. results indicate that a 0.25% carbonyl iron diet is sufficient to induce maximal hepatic hepcidin response. Importantly these results also demonstrate that in a chronic setting of iron administration, the amount of excess hepatic iron may not further influence hepcidin regulation and that expression of hepcidin plateaus at lower hepatic iron levels. These studies provide further insights into the regulation of this import PMID: 29752985
  2. Increased serum hepcidin contributes to the anemia of chronic kidney disease in a murine model. PMID: 27884972
  3. bone marrow transplantation between wild-type and TLR4 knockout mice revealed that hepatic TLR4-dependent hepcidin expression was comparable to macrophage TLR4-dependent hepcidin expression induced by LPS PMID: 29217822
  4. Hepc decreases in Cyp1b1-/- and gestational vitamin A deficiency mice resulted in stellate activation and lipogenesis suppression. PMID: 28583802
  5. data indicate that unlike with many other infections, hepcidin is decreased following M.tb infection, and show that hepcidin ablation does not influence M.tb growth in vivo PMID: 29324800
  6. Hepcidin expression involves epigenetic regulation by histone deacetylase 3. PMID: 28864822
  7. Serum hepcidin levels were measured by competitive ELISA in wild-type and Inhbb-/- mice at baseline and 4 hours after LPS challenge. Although Smad1/5/8 signaling is not activated by inflammatory stimuli in the absence of activin B, this has no impact on the induction of hepcidin expression. PMID: 27903526
  8. hepcidin mRNA upregulation depends on M1 macrophage polarization PMID: 27667162
  9. Our data provide evidence that the interplay of these two hormones could help improve the understanding of the pathogenesis of atherosclerosis and NAFLD. PMID: 29158088
  10. downregulation of hepcidin by siRNA increased iron uptake in bone and liver, which aggravated unloading-induced bone loss. PMID: 27686598
  11. The authors generated mice with cardiomyocyte-specific deletion of hepcidin, or knock-in of hepcidin-resistant ferroportin. They find that while both models maintain normal systemic iron homeostasis, the mice nonetheless develop fatal contractile and metabolic dysfunction as a consequence of cardiomyocyte iron deficiency. PMID: 27897970
  12. Acute tacrolimus treatment transiently increases hepcidin in wild-type mice. FKBP12 preferentially targets the BMP receptor ALK2. ALK2 mutants defective in binding FKBP12 increase hepcidin expression in a ligand-independent manner, through BMP-SMAD signaling. PMID: 28864813
  13. Hamp1 mRNA and plasma hepcidin levels are not good predictors of tissue iron levels, at least in males PMID: 28798083
  14. The lack of effect of erythropoietin on hepcidin expression in mask mice can not be explained by changes in erythroferrone synthesis, as splenic erythroferrone content increased after erythropoietin administration in both C57BL/6 and mask mice. PMID: 29073189
  15. these results characterise a new model of rapidly inducible hepcidin disruption, and demonstrate the critical contribution of hepcidin to the hypoferraemia of inflammation PMID: 27423740
  16. Hepatic gene expression of hepcidin is regulated in beta-thalassemia by ATOH8. PMID: 28405918
  17. Endogenous hepcidin and its agonist mediate resistance to selected infections by clearing non-transferrin-bound iron. PMID: 28465342
  18. the relationship between erythropoiesis and the candidate erythroid regulators, was examined. PMID: 28135344
  19. Smad1 and Smad5 have overlapping functions to govern hepcidin transcription. Moreover, erythropoietin and erythroferrone target Smad1/5 signaling and require Smad1/5 to suppress hepcidin expression. PMID: 28438754
  20. Genetic inactivation of hepatic angiocrine Bmp2 signaling in Stab2-Cre mice caused massive iron overload in the liver and increased serum iron levels and iron deposition in several organs similar to classic hereditary hemochromatosis; these changes were mediated by decreased hepatic expression of hepcidin, a key regulator of iron homeostasis. PMID: 27903529
  21. These studies indicate that drug-like minihepcidins have a potential as future therapeutics for untransfused beta-thalassemia and polycythemia vera. PMID: 27154187
  22. hepcidin induction by endoplasmic reticulum stress involves the central SMAD1/5/8 pathway PMID: 27483343
  23. Increased hepcidin in transferrin-treated thalassemic mice correlates with increased liver BMP2 expression and decreased hepatocyte ERK activation. PMID: 26635037
  24. The combined data presented here highlighted a crucial role of ERFE in regulating hepcidin expression and systematic iron homeostasis under phenylhydrazine-induced hemolytic anemia. PMID: 27067488
  25. The results suggest that physiologic hepcidin levels are insufficient to alter Fpn levels within the retinal pigment epithelium and Muller cells, but may limit iron transport into the retina from vascular endothelial cells. PMID: 26506980
  26. In TNF(DeltaARE/+) and IL-10(-/-)-mice hepatic hepcidin expression and protein content was significantly lower than in corresponding wild-types. PMID: 26302924
  27. In Angiotensin II treated mice, duodenal divalent metal transporter-1 and ferroportin expression levels were increased and hepatic hepcidin mRNA expression and serum hepcidin concentration were reduced. PMID: 25096756
  28. results clarify how commensal bacteria affect hepcidin expression and reveal a novel connection between IL-1beta and activation of BMP signaling. PMID: 26515063
  29. Activation of TLR4 signaling and NF-small ka, CyrillicB are involved in the suppression of hepcidin gene transcription by alcohol in the presence of inflammation in the liver. PMID: 25232250
  30. Study describes extensive blood vessel damage in Alzheimer's disease brain and a reduction in hepcidin and ferroportin levels PMID: 24252754
  31. Hepatic hepcidin plays an important role in sepsis through regulation of iron metabolism PMID: 25264597
  32. Hepc1(-/-) mice had a phenotype of low bone mass and alteration of the bone microarchitecture, most likely caused by a decreased osteoblastic activity PMID: 24652331
  33. Hepcidin mRNA expression was increased in the D-galactose (D-gal) group, decreased in the caloric restriction (CR) group, and was basically unchanged in the D-gal-CR group. PMID: 24044515
  34. results provide evidence that HFE induces hepcidin expression via the BMP pathway: HFE interacts with ALK3 to stabilize ALK3 protein and increase ALK3 expression at the cell surface. PMID: 24904118
  35. Data suggest that hepcidin-1 expression is up-regulated in obesity in visceral/subcutaneous adipose tissue (but down-regulated in liver); hepcidin may play local roles in modulating both iron metabolism and inflammation in adipose tissue. PMID: 24418880
  36. Hepcidin expression is upregulated in interleukin (IL)-10-deficient mice and downregulated in wild-type mice with dextran sulfate sodium-induced colitis. PMID: 24973448
  37. this study shows that the liver-specific KO mice fully recapitulate the severe iron overload phenotype observed in the total KO mice, with increased plasma iron and massive parenchymal iron accumulation. PMID: 24646470
  38. Results indicate a negative role of hepcidin in modulating liver regeneration. PMID: 24123375
  39. The Hamp1 and interleukin-6 knock out genotype resulted in improved erythropoiesis in aged mice. PMID: 23996485
  40. Hepcidin deficiency resulted in a marked reduction of bone load-bearing capacity likely through enhancing bone resorption, suggesting a direct correlation between hepcidin deficiency and bone loss. PMID: 24561287
  41. Hepcidin regulates intrarenal iron handling at the distal nephron. PMID: 23615502
  42. Data suggest that Hamp1 expression in liver can be regulated by dietary factors (here, down-regulated by a hematinic dietary supplement, black soyabean seed coat extract). PMID: 24387766
  43. Identify a link between glucose and iron homeostasis, showing that hepcidin is a gluconeogenic sensor in mice during starvation. PMID: 24361124
  44. Direct transcriptional suppression of hepcidin gene (HAMP) expression mediated by 1,25-dihydroxyvitamin D binding to the vitamin D receptor causes decrease in hepcidin mRNA levels PMID: 24204002
  45. The Brucella abortus model shows multifactorial pathogenesis of inflammatory anemia including iron restriction from increased hepcidin, transient suppression of erythropoiesis, and shortened erythrocyte lifespan. PMID: 24357728
  46. Knockout mice exhibited different patterns in the development and resolution of anemia, supporting the notion that interleukin 6 and hepcidin play distinct roles in modulating erythropoiesis in anemia of inflammation. PMID: 24357729
  47. Exogenous mouse IgG1 Fc fused to the N terminus of mouse IL-22 induced hepcidin production. Ab-mediated blockade of hepcidin partially reversed the effects on iron biology caused by IL-22R stimulation. PMID: 23836059
  48. Iron overload increased pro-hepcidin that remained high in hypoxia. PMID: 23656253
  49. A model is proposed that suggests that unlike proteases, which are irreversibly bound to activated alpha2M, hepcidin remains labile and available to down-regulate Fpn1. PMID: 23846698
  50. role of hepcidin in the setting of hypoferremia during acute inflammation PMID: 23637785

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Subcellular Location
Secreted.
Protein Families
Hepcidin family
Tissue Specificity
Highly expressed in the liver and to a much lesser extent in the heart. Secreted in blood.
Database Links
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