Code | CSB-E14395m |
Size | 96T,5×96T,10×96T |
Price | Request a Quote or Start an on-line Chat |
Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<10% | ||||||
Three samples of known concentration were tested in twenty assays to assess. | ||||||
To assess the linearity of the assay, samples were spiked with high concentrations of mouse Hepc in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
Sample | Serum(n=4) | |||||
1:1 | Average % | 87 | ||||
Range % | 83-91 | |||||
1:2 | Average % | 90 | ||||
Range % | 85-96 | |||||
1:4 | Average % | 94 | ||||
Range % | 89-99 | |||||
1:8 | Average % | 97 | ||||
Range % | 93-102 |
The recovery of mouse Hepc spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 93 | 90-96 | ||||
EDTA plasma (n=4) | 99 | 94-103 | ||||
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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This mouse hepcidin (HAMP) ELISA kit employs the quantitative sandwich enzyme immunoassay technique to measure the levels of mouse hepcidin in multiple samples, including serum, plasma, or tissue homogenates. The enzyme-substrate chromogenic reaction is also used to amplify the signal and quantify the levels of the analyte through the intensity of the colored product. The color intensity positively correlates with the amount of hepcidin bound in the initial step.
Hepcidin is an endogenous antimicrobial peptide that is produced by the liver and mainly functions to negatively regulate iron. It controls plasma iron levels by regulating the intestinal absorption of dietary iron, as well as the release of iron from macrophages and the transfer of iron stored in the hepatocytes. Hepcidin induces ferroportin internalization and degradation in target cells, mostly macrophages and enterocytes, leading to iron sequestration in macrophages and declined iron absorption from the intestine. The expression of hepcidin is induced by elevated iron concentration and inflammation but inhibited by anemia and hypoxia. Dysregulation of hepcidin is associated with iron disorder-related diseases, such as anemias, hereditary hemochromatosis, and β-thalassemia.
Sample type: Serum
Sample species: Mouse
Sample dilution: No dilution
Review: I have used this kit to evaluate the serum hepcidin levels in diseased and normal mice.
By Anonymous