Mouse macrophage inflammatory protein 3β,MIP-3β ELISA kit

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Mouse macrophage inflammatory protein 3β,MIP-3β ELISA kit

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Citations (2) Protocol MSDS

Code	CSB-E04671m
Size	96T,5×96T,10×96T
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Citations (2)
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Alternative Names

Ccl19 ELISA Kit; Elc ELISA Kit; Scya19 ELISA Kit; C-C motif chemokine 19 ELISA Kit; Epstein-Barr virus-induced molecule 1 ligand chemokine ELISA Kit; EBI1 ligand chemokine ELISA Kit; ELC ELISA Kit; Small-inducible cytokine A19 ELISA Kit

Abbreviation

CCL19

Uniprot No.

O70460

Species

Mus musculus (Mouse)

Sample Types

serum, plasma, tissue homogenates

Detection Range

39 pg/ml - 2500 pg/ml

Sensitivity

9.75 pg/ml

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse MIP-3β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	96
	Range %	93-99
1:2	Average %	104
	Range %	100-107
1:4	Average %	85
	Range %	81-89
1:8	Average %	98
	Range %	94-103

Recovery

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml

OD1

OD2

Average

Corrected

2500

2.830

2.786

2.808

2.629

1250

2.286

2.301

2.294

2.115

625

1.656

1.672

1.664

1.485

312.5

1.007

1.069

1.038

0.859

156.25

0.683

0.698

0.691

0.512

0.457

0.438

0.448

0.269

0.321

0.331

0.326

0.147

0.178

0.179

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Description

This Mouse CCL19 ELISA Kit was designed for the quantitative measurement of Mouse CCL19 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 39 pg/ml - 2500 pg/ml and the sensitivity is 9.75 pg/ml.

Ginsenoside CK potentiates SIRT1 to alleviate lupus nephritis through compensating for XBP1-mediated endoplasmic reticulum stress in plasma cells Z Song, Y Li, S Xu, S Qian, W Xu, L Xu, F Tian,Journal of Pharmaceutical Analysis,2025
Reciprocal regulation of SIRT1 and AMPK by Ginsenoside compound K impedes the conversion from plasma cells to mitigate for podocyte injury in MRL/lpr mice in a B cell-specific manner Z Song,Journal of ginseng research,2024

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Function
(From Uniprot)

Strongly chemotactic for naive (L-selectinhi) CD4 T-cells and for CD8 T-cells and weakly attractive for resting B-cells and memory (L-selectinlo) CD4 T-cells. May play a role in promoting encounters between recirculating T-cells and dendritic cells and in the migration of activated B-cells into the T-zone of secondary lymphoid tissues. Binds to chemokine receptor CCR7. Binds to atypical chemokine receptor ACKR4 and mediates the recruitment of beta-arrestin (ARRB1/2) to ACKR4.

Subcellular Location

Secreted.

Protein Families

Intercrine beta (chemokine CC) family

Tissue Specificity

Highly expressed by dendritic cells in mesenteric and peripheral lymph nodes. Significant expression in spleen (T cell zone or periarteriolar lymphatic sheath) and Peyer patches. Low expression in thymus.

Database Links

KEGG: mmu:100039053

STRING: 10090.ENSMUSP00000137353

UniGene: Mm.426373

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Related pathways

Chemokine signaling pathway NF-kappa B signaling pathway

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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