Mouse secretory immunoglobulin A,sIgA ELISA Kit

Secretory immunoglobulin A (sIgA) represents the predominant antibody class in mucosal secretions, serving as the first line of immune defense at mucosal surfaces including the respiratory, gastrointestinal, and urogenital tracts. Plasma cells in mucosal tissues produce this dimeric immunoglobulin, which is then transported across epithelial barriers through the polymeric immunoglobulin receptor. During transport, it acquires a secretory component that enhances its stability and function. sIgA plays essential roles in pathogen neutralization, immune exclusion, and maintaining mucosal homeostasis. These properties make it an important biomarker for mucosal immunity research and disease studies.

The Mouse secretory immunoglobulin A ELISA Kit (CSB-E08413m) uses a quantitative sandwich assay principle for measuring sIgA levels in mouse samples. This kit works with multiple sample types including serum, plasma, tissue homogenates, and bronchoalveolar lavage fluid. The detection range spans 15.6-1000 ng/mL with sensitivity of 3.9 ng/mL. The assay requires 50-100 μL sample volume, operates with detection at 450 nm wavelength, and can be completed within 1-5 hours.

Application Examples

Note: The following application examples are drawn from a selection of publications citing this product. For additional applications, please refer to the full list of references in the "Citations" section.

This ELISA kit has been used in research examining immune responses and mucosal immunity in mouse models. Studies have applied the kit to quantify secretory immunoglobulin A levels in lung tissue homogenates, providing insights into local immune function and respiratory health.

• Respiratory research: Measuring mucosal immune responses through secretory immunoglobulin A levels in lung tissue samples
• Immune function studies: Quantifying secretory antibody levels to evaluate local immune status in experimental mouse models
• Tissue-based analysis: Application in homogenized tissue samples to examine organ-specific immune responses