Pig Angiopoietin 2,ANG-2 ELISA Kit

Code CSB-E08460p
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
angiopoietin 2
Alternative Names
ANGPT2Angiopoietin-2 ELISA kit; ANG-2 ELISA kit
Abbreviation
ANGPT2
Uniprot No.
Species
Sus scrofa (Pig)
Sample Types
serum, plasma, tissue homogenates
Detection Range
0.94 ng/mL-60 ng/mL
Sensitivity
0.235 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cardiovascular
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of pig ANG-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %86
Range %83-89
1:2Average %99
Range %96-102
1:4Average %87
Range %84-90
1:8Average %97
Range %91-103
Recovery
The recovery of pig ANG-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 8985-92
EDTA plasma (n=4)9387-98
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
602.509 2.596 2.553 2.401
301.749 1.768 1.759 1.607
151.004 1.068 1.036 0.884
7.50.663 0.642 0.653 0.501
3.750.390 0.378 0.384 0.232
1.880.298 0.290 0.294 0.142
0.940.224 0.239 0.232 0.080
00.151 0.153 0.152
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Pig ANGPT2 ELISA Kit was designed for the quantitative measurement of Pig ANGPT2 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.94 ng/mL-60 ng/mL and the sensitivity is 0.235 ng/mL.

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Target Background

Function
(From Uniprot)
Binds to TEK/TIE2, competing for the ANGPT1 binding site, and modulating angiogenic signals mediated by ANGPT1. Can induce tyrosine phosphorylation of TEK/TIE2 in the absence of ANGPT1. In the absence of angiogenic inducers, such as VEGF, ANGPT2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal.
Gene References into Functions
  1. expression of angiopoietin 2 in the porcine metanephric kidney was observed in the podocytes of early developing glomeruli, but not in the cells near the glomerular hilus PMID: 20598355
  2. ANGPT2 remained upregulated during maturation of glomeruli, which may be explained by the continuous growth of the glomeruli, as observed by stereological examination. PMID: 20713984
Subcellular Location
Secreted.
Database Links
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