ANGPT2 Research Reagents

Angiopoietin-2 is a protein in humans that is encoded by ANGPT2 gene with a molecular mass of about 75kDa. This protein is about 60% identical to ANGPT1. The homology of ANGPT1 between humans and mice is as high as 97.6%. ANGPT1 is mainly expressed in myocardium, and later expressed in perivascular cells including peritubular cells, vascular smooth muscle cells and tumor cells. ANGPT2 is mainly secreted by endothelial cells. It is a natural competitive antagonist of ANGPT1.

ANGPT2 can competitively inhibit the phosphorylation of ANGPT1 to promote TIE2. It is mainly expressed in vascular endothelial cells and maintain the dynamic equilibrium of vascular growth and degeneration by inhibiting the activation of ANGPT1. In addition, the expression of ANGPT2 can be regulated by a variety of factors such as VEGF, EGF, bFGF, TNFα, etc. Hypoxia can promote the expression of ANGPT2, while ANGPT1 and TGF-β can down-regulate its expression level. The effect of ANGPT2 on angiogenesis is related to the local microenvironment. In the presence of VEGF, ANGPT2 can antagonize the effect of ANGPT1 on promoting vascular structure stability, which is beneficial to vascular sprouting and growth; when VEGF is lacking, ANGPT2 inhibits ANGPT1, which is beneficial to vascular regression.

Popular Product

Recombinant Human Angiopoietin-2(ANGPT2) (Active) (CSB-MP001707HU(A4))

Validated Data

Purity determined
by SDS-PAGE

Greater than 92% as determined by SDS-PAGE.

The binding activity of antigen-antibody validated by functional ELISA

Immobilized Human ANGPT2 at 2 μg/ml can bind anti-ANGPT2 recombinant antibody (CSB-RA001707MA01HU), the EC₅₀ is 0.6666-0.8876 ng/mL.