Pig Brain-derived neurotrophic factor(BDNF) ELISA kit

Instructions
Code CSB-EL002655PI
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name brain-derived neurotrophic factor
Alternative Names BDNFBrain-derived neurotrophic factor ELISA kit; BDNF) [Cleaved into: BDNF precursor form ELISA kit; ProBDNF)] ELISA kit
Abbreviation BDNF
Uniprot No. P14082
Species Sus scrofa (Pig)
Sample Types serum, plasma, tissue homogenates
Detection Range 9.375 ng/mL-600 ng/mL
Sensitivity 2.35 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of pig BDNF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 96
Range % 91-102
1:2 Average % 102
Range % 97-107
1:4 Average % 109
Range % 105-113
1:8 Average % 103
Range % 96-107
Recovery
The recovery of pig BDNF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 102 98-105
EDTA plasma (n=4) 86 93-92
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
600 2.134 2.081 2.108 2.016
300 1.537 1.623 1.580 1.488
150 1.167 1.123 1.145 1.053
75 0.769 0.774 0.772 0.680
37.5 0.513 0.503 0.508 0.416
18.75 0.353 0.344 0.349 0.257
9.375 0.227 0.221 0.224 0.132
0 0.091 0.093 0.092  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

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Target Background

Function
(From Uniprot)
During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS (By similarity).
Gene References into Functions
  1. measurement of brain derived neurotrophic factor in serum brings a practical approach to study the effects of environmental enrichment on neurobiological changes in domestic animals PMID: 30007130
  2. Lf upregulated several canonical signaling pathways associated with neurodevelopment and cognition and influenced ~10 genes involved in the brain-derived neurotrophin factor (BDNF) signaling pathway. PMID: 25146846
  3. Taken together, these data indicate that recurrent tethering stress in sows over 4.5 years results in a loss of neurotrophic support by BDNF, mediated by an overactive neuroendocrine system. PMID: 22584108
  4. FiO2 used for resuscitation affects matrix metalloproteinases MMP-9 and MMP-2, caspase-3 and BDNF PMID: 21151608
Subcellular Location Secreted
Protein Families NGF-beta family
Tissue Specificity Brain and central nervous system.
Database Links

KEGG: ssc:397495

STRING: 9823.ENSSSCP00000014166

UniGene: Ssc.16243

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