Pig basic fibroblast growth factor,bFGF ELISA Kit

Code CSB-E12841p
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
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Product Details

Target Name
fibroblast growth factor 2 (basic)
Alternative Names
FGF2Fibroblast growth factor 2 ELISA Kit; FGF-2 ELISA Kit; Basic fibroblast growth factor ELISA Kit; bFGF ELISA Kit; Heparin-binding growth factor 2 ELISA Kit; HBGF-2 ELISA Kit; Prostatic growth factor ELISA Kit; Fragments ELISA Kit
Abbreviation
Uniprot No.
Species
Sus scrofa (Pig)
Sample Types
serum, plasma, tissue homogenates
Detection Range
4.7 pg/mL-300 pg/mL
Sensitivity
1.2 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cardiovascular
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of pig bFGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 88  
Range % 80-100  
1:2 Average % 97  
Range % 91-105  
1:4 Average % 94  
Range % 90-110  
1:8 Average % 95  
Range % 82-105  
Recovery
The recovery of pig bFGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 95 85-104  
EDTA plasma (n=4) 98 92-106  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
300 2.175 2.157 2.166 1.966  
150 1.582 1.612 1.597 1.397  
75 1.202 1.134 1.168 0.968  
37.5 0.628 0.603 0.616 0.416  
18.75 0.377 0.362 0.370 0.170  
9.4 0.296 0.301 0.299 0.099  
4.7 0.265 0.256 0.261 0.061  
0 0.196 0.204 0.200    
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Pig FGF2 ELISA Kit was designed for the quantitative measurement of Pig FGF2 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 4.7 pg/mL-300 pg/mL and the sensitivity is 1.2 pg/mL.

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Target Background

Function
(From Uniprot)
Acts as a ligand for FGFR1, FGFR2, FGFR3 and FGFR4. Also acts as an integrin ligand which is required for FGF2 signaling. Binds to integrin ITGAV:ITGB3. Plays an important role in the regulation of cell survival, cell division, cell differentiation and cell migration. Functions as a potent mitogen in vitro. Can induce angiogenesis. Mediates phosphorylation of ERK1/2 and thereby promotes retinal lens fiber differentiation.
Subcellular Location
Secreted. Nucleus.
Protein Families
Heparin-binding growth factors family
Database Links
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