Pig monocyte chemotactic protein 1,MCP1 ELISA Kit

Code CSB-E13178p
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Description

This Pig CCL2 ELISA Kit was designed for the quantitative measurement of Pig CCL2 protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.81 pg/mL.

Target Name chemokine (C-C motif) ligand 2
Alternative Names CCL2 ELISA Kit; SCYA2C-C motif chemokine 2 ELISA Kit; Monocyte chemoattractant protein 1 ELISA Kit; Monocyte chemotactic protein 1 ELISA Kit; MCP-1 ELISA Kit; Small-inducible cytokine A2 ELISA Kit
Abbreviation CCL2
Uniprot No. P42831
Species Sus scrofa (Pig)
Sample Types serum, plasma
Detection Range 31.25 pg/mL-2000 pg/mL
Sensitivity 7.81 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of pig MCP1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %92
Range %87-96
1:2Average %98
Range %93-102
1:4Average %85
Range %81-89
1:8Average %96
Range %93-99
Recovery
The recovery of pig MCP1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9188-96
EDTA plasma (n=4)8580-90
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
20002.078 2.069 2.074 1.880
10001.610 1.687 1.649 1.455
5001.099 1.059 1.079 0.885
2500.615 0.638 0.627 0.433
1250.427 0.438 0.433 0.239
62.50.342 0.349 0.346 0.152
31.250.276 0.268 0.272 0.078
00.189 0.198 0.194
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
Acts as a ligand for C-C chemokine receptor CCR2. Signals through binding and activation of CCR2 and induces a strong chemotactic response and mobilization of intracellular calcium ions. Exhibits a chemotactic activity for monocytes and basophils but not neutrophils or eosinophils. Plays an important role in mediating peripheral nerve injury-induced neuropathic pain. Increases NMDA-mediated synaptic transmission in both dopamine D1 and D2 receptor-containing neurons, which may be caused by MAPK/ERK-dependent phosphorylation of GRIN2B/NMDAR2B.
Gene References into Functions
  1. these results provide novel insights into CCL2-mediated signaling mechanisms in the porcine endometrium at the maternal-fetal interface during early pregnancy PMID: 29278679
  2. CXCL10 protein was detected in serum from pigs infected with a high virulence African swine fever virus isolate. Levels of CCL2 mRNA were increased in pigs infected with the high virulence isolate compared to a low virulence isolate. PMID: 24083897
  3. Porcine CCL2 binding on Tuk4+SLA-II- but not on Tuk4-SLA-II+ cells was detected by using a CCL2-green fluorescence protein fusion protein. PMID: 20670605
  4. The protective properties of flavonoids, such as EGCG, against endothelial inflammation may be regulated in part though induction of HO-1 and subsequent activator protein-1 signaling. PMID: 20580034
  5. MCP-1 contributes to functional and structural impairment in the kidney in renal artery stenosis, mainly in the tubulointerstitial compartment PMID: 19730125
  6. may contribute to xenograft rejection by attracting monocytes into the islets PMID: 14962280
  7. Contrast media did not affect bone marrow cell viability/MCP-1 secretion. PMID: 15274157
  8. Porcine vessel wall injury via balloon arthroplasty upregulates expression of CCL2 by coronary artery transmural and perivascular cells in a sequential pattern. PMID: 16926016
  9. in the aortic arch expression of monocyte chemoattractant protein 1 (MCP-1) was upregulated, compared with the thoracic aorta PMID: 17588585
  10. Variable gene expression (eg, matrix metalloproteinase-9, CCL2 and Lp-PLA(2) mRNAs), both in regard to the arterial bed and duration of disease, was associated with variable plaque development and progression. PMID: 18276914
  11. myocardial infarction induced proinflammatory gene and protein expression in peripheral blood mononuclear cells of tissue factor cyclo-oxygenase-2, monocyte chemoattractant protein-1 and CRP PMID: 19036073

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Subcellular Location Secreted.
Protein Families Intercrine beta (chemokine CC) family
Database Links

KEGG: ssc:397422

STRING: 9823.ENSSSCP00000018779

UniGene: Ssc.657

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