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Rabbit Interleukin 1,IL-1 ELISA Kit

Citations (6) Protocol MSDS
Code CSB-E06896Rb
Size 96T,5×96T,10×96T
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Product Details

Target Name
Interleukin 1,IL-1
Alternative Names
IL1A ELISA Kit; Interleukin-1 alpha ELISA Kit; IL-1 alpha ELISA Kit
Abbreviation
IL1A
Uniprot No.
P04822
Species
Oryctolagus cuniculus (Rabbit)
Sample Types
serum, plasma, tissue homogenates
Detection Range
15.6 pg/mL-1000 pg/mL
Sensitivity
3.9 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rabbit IL-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
　SampleSerum(n=4)
1:1Average %88
Range %84-96
1:2Average %92
Range %85-100
1:4Average %95
Range %89-101
1:8Average %93
Range %87-99
Recovery
The recovery of rabbit IL-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9688-99
EDTA plasma (n=4)9284-102
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
10002.772 2.641 2.707 2.547
5002.216 2.068 2.142 1.982
2501.286 1.186 1.236 1.076
1250.652 0.634 0.643 0.483
62.50.370 0.355 0.363 0.203
31.20.282 0.264 0.273 0.113
15.60.199 0.212 0.206 0.046
00.164 0.156 0.160 　
ELISA Data Analysis
ELISA Standard Curve & Curve Expert software
Troubleshooting
and FAQs
ELISA kit FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Description

The rabbit IL-1 ELISA kit is suitable for the quantitative determination of rabbit IL-1 in different sample types, including serum, plasma, and tissue homogenates. This assay employs the bi-antibody sandwich technique and enzyme-substrate chromogenic reaction to quantify antigen levels in the sample. The amount of synthesized colored products is positively related to the analyte of interest in the sample.

IL-1, also called IL-1α or IL1A, is a ubiquitous and pivotal pro-inflammatory cytokine. IL-1/IL-1R1 signaling induces pro-inflammatory signaling cascades including NF-κB, JNK, p38, AP-1, and MAPK pathways, resulting in the expression of myriad mediators, which orchestrate the development of an immune response. It exerts both beneficial, promoting innate immunity against invading microorganisms, and harmful roles in a variety of autoimmune and autoinflammatory diseases including cancer. In cancers, IL-1 affects the tumor microenvironment and promotes the initiation and progression of tumor cells.

Citations

Related Products

IL1A Proteins

IL1A ELISA kits

Customer Reviews and Q&A

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Target Background

Function
(From Uniprot)
Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.
Gene References into Functions
  1. We propose that induction of collagenase-1 by IL-1alpha in both WF and NF depends on a unique combination of cell type-specific signaling pathways. PMID: 17455222
Subcellular Location
Cytoplasm. Secreted.
Protein Families
IL-1 family
Database Links
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