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| Code | CSB-E06896Rb |
| Size | 96T,5×96T,10×96T |
| Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
| Have Questions? | Leave a Message or Start an on-line Chat |
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
| To assess the linearity of the assay, samples were spiked with high concentrations of rabbit IL-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 88 | ||||
| Range % | 84-96 | |||||
| 1:2 | Average % | 92 | ||||
| Range % | 85-100 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 89-101 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 87-99 | |||||
| The recovery of rabbit IL-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 88-99 | ||||
| EDTA plasma (n=4) | 92 | 84-102 | ||||
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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The rabbit IL-1 ELISA kit is suitable for the quantitative determination of rabbit IL-1 in different sample types, including serum, plasma, and tissue homogenates. This assay employs the bi-antibody sandwich technique and enzyme-substrate chromogenic reaction to quantify antigen levels in the sample. The amount of synthesized colored products is positively related to the analyte of interest in the sample.
IL-1, also called IL-1α or IL1A, is a ubiquitous and pivotal pro-inflammatory cytokine. IL-1/IL-1R1 signaling induces pro-inflammatory signaling cascades including NF-κB, JNK, p38, AP-1, and MAPK pathways, resulting in the expression of myriad mediators, which orchestrate the development of an immune response. It exerts both beneficial, promoting innate immunity against invading microorganisms, and harmful roles in a variety of autoimmune and autoinflammatory diseases including cancer. In cancers, IL-1 affects the tumor microenvironment and promotes the initiation and progression of tumor cells.
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