Code | CSB-E06896Rb |
Size | 96T,5×96T,10×96T |
Price | Request a Quote |
Trial Size |
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Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<10% | ||||||
Three samples of known concentration were tested in twenty assays to assess. | ||||||
To assess the linearity of the assay, samples were spiked with high concentrations of rabbit IL-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
Sample | Serum(n=4) | |||||
1:1 | Average % | 88 | ||||
Range % | 84-96 | |||||
1:2 | Average % | 92 | ||||
Range % | 85-100 | |||||
1:4 | Average % | 95 | ||||
Range % | 89-101 | |||||
1:8 | Average % | 93 | ||||
Range % | 87-99 |
The recovery of rabbit IL-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 96 | 88-99 | ||||
EDTA plasma (n=4) | 92 | 84-102 | ||||
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Interleukin-1 (IL-1) is a pro-inflammatory cytokine that plays a central role in immune responses and inflammatory processes. This cytokine family includes IL-1α and IL-1β, which are produced primarily by activated macrophages, monocytes, and other immune cells in response to various stimuli including pathogens and tissue damage. IL-1 mediates numerous biological effects including fever induction, acute-phase protein synthesis, and activation of T-helper cells. It serves as an important mediator in both innate and adaptive immunity.
The Rabbit Interleukin 1,IL-1 ELISA Kit (CSB-E06896Rb) is designed for quantitative measurement of IL-1 in Oryctolagus cuniculus samples. This sandwich ELISA works with serum, plasma, and tissue homogenates. The detection range spans 15.6 pg/mL to 1000 pg/mL with sensitivity of 3.9 pg/mL. The assay requires 50-100 μL sample volume and completes within 1-5 hours. Detection occurs at 450 nm wavelength for reliable quantification of rabbit IL-1 levels.
Application Examples
Note: The following application examples are drawn from a selection of publications citing this product. For additional applications, please refer to the full list of references in the "Citations" section.
This ELISA kit has been used in research examining inflammatory responses and cytokine profiling across different biological sample types. Studies have applied the kit to quantify interleukin levels in serum samples and aqueous humor, supporting research into inflammatory processes and immune system activity.
• Inflammatory cytokine profiling - Used alongside other cytokine assays to evaluate inflammatory marker panels in serum samples
• Ocular inflammation research - Applied to measure cytokine concentrations in aqueous humor samples to examine inflammatory responses in eye-related studies
• Multi-analyte biomarker studies - Incorporated into broader cytokine panels that include various interleukins and inflammatory mediators for comprehensive immune response characterization
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