Rat Interleukin 1α,IL-1α ELISA kit

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Rat Interleukin 1α,IL-1α ELISA kit

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Citations (6) Protocol MSDS

Code	CSB-E04622r
Size	96T,5×96T,10×96T
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Product Details
Citations (6)
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Alternative Names

IL1A ELISA Kit; Interleukin-1 alpha ELISA Kit; IL-1 alpha ELISA Kit

Abbreviation

IL1A

Uniprot No.

P04822

Species

Rattus norvegicus (Rat)

Sample Types

serum, plasma, tissue homogenates.

Detection Range

78 pg/ml-5000 pg/ml.

Sensitivity

19.5 pg/ml.

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-1α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	96
	Range %	92-102
1:2	Average %	98
	Range %	91-104
1:4	Average %	101
	Range %	98-107
1:8	Average %	89
	Range %	84-96

Recovery

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml

OD1

OD2

Average

Corrected

5000

2.657

2.556

2.607

2.425

2500

2.123

2.022

2.073

1.891

1250

1.468

1.447

1.458

1.276

625

0.927

0.916

0.922

0.740

312

0.568

0.579

0.574

0.392

156

0.415

0.404

0.410

0.228

0.312

0.300

0.306

0.124

0.180

0.183

0.182

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Description

This Rat IL1A ELISA Kit was designed for the quantitative measurement of Rat IL1A protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 1.56 pg/mL-100 pg/mL and the sensitivity is 0.39 pg/mL .

Appraisal of the Pre-Emptive Effect of Lactoferrin Against Chromium-Induced Testicular Toxicity in Male Rats SS Abd El-Rahman,Biological trace element research,2023
Hepatoprotective Potential of Genkwanin Against Aflatoxin B1-Induced Biochemical, Inflammatory and Histopathological Toxicity in Rats MU Ijaz,Pakistan Veterinary Journal,2022
Electroacupuncture Prevents Osteoarthritis of High-Fat Diet-Induced Obese Rats X Lin-Lin,BIOMED RESEARCH INTERNATIONAL,2020
Volatile Oil of Amomum villosum Inhibits Nonalcoholic Fatty Liver Disease via the Gut-Liver Axis Shanhong Lu.et al,BioMed Research International,2018
Magnesium isoglycyrrhizinate inhibits inflammatory response through STAT3 pathway to protect remnant liver function Tang GH. et al,World J Gastroenterol,2015
Ethyl-p-methoxycinnamate isolated from kaempferia galanga inhibits inflammation by suppressing interleukin-1, tumor necrosis factor-a, and angiogenesis by blocking endothelial functions. Umar MI et al,Clinics,2014

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Function
(From Uniprot)

Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.

Gene References into Functions

We propose that induction of collagenase-1 by IL-1alpha in both WF and NF depends on a unique combination of cell type-specific signaling pathways. PMID: 17455222

Subcellular Location

Cytoplasm. Secreted.

Protein Families

IL-1 family

Database Links

KEGG: ocu:100009250

STRING: 9986.ENSOCUP00000007573

UniGene: Ocu.2080

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Email: [email protected]
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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

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Related pathways

MAPK signaling pathway Necroptosis Cellular senescence Hematopoietic cell lineage Osteoclast differentiation AGE-RAGE signaling pathway in diabetic complications

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