Rabbit cardiac troponin Ⅰ,cTn-ⅠELISA Kit

Instructions
Code CSB-E11286Rb
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name troponin I type 3 (cardiac)
Alternative Names TNNI3 ELISA Kit; Troponin I ELISA Kit; cardiac muscle ELISA Kit; Cardiac troponin I ELISA Kit
Abbreviation TNNI3
Uniprot No. P02646
Species Oryctolagus cuniculus (Rabbit)
Sample Types serum, plasma, tissue homogenates
Detection Range 47 pg/mL-3000 pg/mL
Sensitivity 11.75 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rabbit cTn-Ⅰ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %87
Range %81-92
1:2Average %96
Range %92-99
1:4Average %94
Range %89-98
1:8Average %92
Range %88-96
Recovery
The recovery of rabbit cTn-Ⅰ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 8783-94
EDTA plasma (n=4)8985-97
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
30002.754 2.559 2.657 2.564
15002.115 2.020 2.068 1.975
7501.248 1.227 1.238 1.145
3750.658 0.677 0.668 0.575
187.50.403 0.412 0.408 0.315
940.264 0.278 0.271 0.178
470.151 0.147 0.149 0.056
00.093 0.092 0.093
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Target Data

Function Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
Protein Families Troponin I family

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