Rabbit monocyte chemotactic protein 1/monocyte chemotactic and activating factor,MCP1/MCAF ELISA kit

Code CSB-E06921Rb
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Description

This Rabbit CCL2 ELISA Kit was designed for the quantitative measurement of Rabbit CCL2 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 23.5 pg/mL-1500 pg/mL and the sensitivity is 5.87 pg/mL .

Target Name chemokine (C-C motif) ligand 2
Alternative Names CCL2 ELISA Kit; SCYA2C-C motif chemokine 2 ELISA Kit; Monocyte chemoattractant protein 1 ELISA Kit; Monocyte chemotactic protein 1 ELISA Kit; MCP-1 ELISA Kit; Small-inducible cytokine A2 ELISA Kit
Abbreviation CCL2
Uniprot No. P28292
Species Oryctolagus cuniculus (Rabbit)
Sample Types serum, plasma, tissue homogenates
Detection Range 23.5 pg/mL-1500 pg/mL
Sensitivity 5.87 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rabbit MCP1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 94  
Range % 90-100  
1:2 Average % 95  
Range % 91-100  
1:4 Average % 95  
Range % 89-101  
1:8 Average % 97  
Range % 91-100  
Recovery
The recovery of rabbit MCP1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 96 91-99  
EDTA plasma (n=4) 98 94-104  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
1500 2.488 2.501 2.495 2.322  
750 1.985 1.999 1.992 1.819  
375 1.483 1.471 1.477 1.304  
187.5 0.938 0.942 0.940 0.767  
94 0.574 0.584 0.579 0.406  
47 0.382 0.396 0.389 0.216  
23.5 0.251 0.253 0.252 0.079  
0 0.174 0.172 0.173    
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
Acts as a ligand for C-C chemokine receptor CCR2. Signals through binding and activation of CCR2 and induces a strong chemotactic response and mobilization of intracellular calcium ions. Exhibits a chemotactic activity for monocytes and basophils but not neutrophils or eosinophils. Plays an important role in mediating peripheral nerve injury-induced neuropathic pain. Increases NMDA-mediated synaptic transmission in both dopamine D1 and D2 receptor-containing neurons, which may be caused by MAPK/ERK-dependent phosphorylation of GRIN2B/NMDAR2B.
Gene References into Functions
  1. Data show that peripheral blood endothelial progenitor cells can increase the expressions of chemokines CCL2 and CXCL12 and promote the migration of enhanced green fluorescent protein-positive bone marrow stromal cells to bone defect site. PMID: 26728372
  2. Data show that the serum levels of mast cell tryptase, monocyte chemoattractant protein-1 (MCP-1) and interleukin-8 (IL-8) decreased significantly in Dachengqi decoction treatment group. PMID: 25270202
  3. measurements of CCL2 and macrophages can serve as new inflammatory biomarkers to evaluate the safety of promising novel microbicides for prevention of HIV. PMID: 19929801
  4. Expression of MCP-1 was successfully inhibited by transfecting MCP-1 siRNA expression plasmid to the carotid artery, and the progression of atherosclerosis was restricted by RNAi-mediated silencing of MCP-1 expression. PMID: 19467827
Subcellular Location Secreted.
Protein Families Intercrine beta (chemokine CC) family
Database Links

KEGG: ocu:100009130

STRING: 9986.ENSOCUP00000015636

UniGene: Ocu.1921

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