Rabbit monocyte chemotactic protein 1/monocyte chemotactic and activating factor,MCP1/MCAF ELISA kit

Code CSB-E06921Rb
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P28292
Lead Time 3-5 working days
Protein Biological Process 2 chemokine
Abbreviation CCL2
Protein Biological Process 1 Immunity
Target Name chemokine (C-C motif) ligand 2
Alias GDCF-2, HC11, HSMCR30, MCAF, MCP-1, MCP1, MGC9434, SCYA2, SMC-CF, monocyte chemoattractant protein-1|monocyte chemotactic and activating factor|monocyte secretory protein JE|small inducible cytokine
Species Oryctolagus cuniculus (Rabbit)
Protein Biological Process 3 Chemotaxis
Sample Types serum, plasma, tissue homogenates
Detection Range 23.5 pg/mL-1500 pg/mL
Sensitivity 5.87 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. This protein is structurally related to the CXC subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4.
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rabbit MCP1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 94  
Range % 90-100  
1:2 Average % 95  
Range % 91-100  
1:4 Average % 95  
Range % 89-101  
1:8 Average % 97  
Range % 91-100  
Recovery
The recovery of rabbit MCP1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 96 91-99  
EDTA plasma (n=4) 98 94-104  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
1500 2.488 2.501 2.495 2.322  
750 1.985 1.999 1.992 1.819  
375 1.483 1.471 1.477 1.304  
187.5 0.938 0.942 0.940 0.767  
94 0.574 0.584 0.579 0.406  
47 0.382 0.396 0.389 0.216  
23.5 0.251 0.253 0.252 0.079  
0 0.174 0.172 0.173    
References
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Function Chemotactic factor that attracts monocytes, but not neutrophils.
Subcellular Location Secreted
Protein Families Intercrine beta (chemokine CC) family
Database Links

KEGG: ocu:100009130

STRING: 9986.ENSOCUP00000015636

UniGene: Ocu.1921

Pathway Chemokine signaling pathway
TNF signaling pathway
IL-17 signaling pathway
NOD-like receptor signaling pathway
AGE-RAGE signaling pathway in diabetic complications

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