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Rat Cathepsin K,cath-K ELISA Kit

Rat Cathepsin K,cath-K ELISA Kit

Citations (3) Protocol

Code	CSB-E09439r
Size	96T,5×96T,10×96T
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Product Details
Citations (3)
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Target Background

Product Details

Description

This Rat CTSK ELISA Kit was designed for the quantitative measurement of Rat CTSK protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.

Target Name

cathepsin K

Alternative Names

Ctsk ELISA Kit; Cathepsin K ELISA Kit; EC 3.4.22.38 ELISA Kit

Abbreviation

CTSK

Uniprot No.

O35186

Species

Rattus norvegicus (Rat)

Sample Types

serum, plasma, tissue homogenates

Detection Range

15.6 pg/mL-1000 pg/mL

Sensitivity

3.9 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Signal Transduction

Assay Principle

quantitative

Measurement

Sandwich

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat cath-K in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	104
	Range %	100-107
1:2	Average %	99
	Range %	95-103
1:4	Average %	85
	Range %	80-90
1:8	Average %	107
	Range %	103-110

Recovery

The recovery of rat cath-K spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	86	83-89
EDTA plasma (n=4)	95	90-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.801	2.765	2.783	2.682
500	2.342	2.204	2.273	2.172
250	1.343	1.406	1.375	1.274
125	0.679	0.701	0.690	0.589
62.5	0.399	0.405	0.402	0.301
31.2	0.251	0.242	0.247	0.146
15.6	0.166	0.164	0.165	0.064
0	0.102	0.100	0.101

ELISA Data Analysis

Watch ELISA data processing video & download Curve Expert if needed

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

Citations

Fructus Ligustri Lucidi preserves bone quality through induction of canonical Wnt/β‐catenin signaling pathway in ovariectomized rats H Liu,Phytother Res,2020
Early changes in serum osteocalcin and body weight are predictive of implant fixation in a rat model of implant loosening Wilson B M, et al,Journal of Orthopaedic Research,2019
Research article Preventive effect of dried plum extract against dexamethasone-induced osteoporosis in male rats through inhibiting cathepsin-K activity, lipogenesis and trabecular bone loss Azza M. El-Wakf,Journal of Innovations in Pharmaceutical and Biological Sciences,2019

Customer Reviews and Q&A

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Target Background

Function (From Uniprot)	Thiol protease involved in osteoclastic bone resorption and may participate partially in the disorder of bone remodeling. Displays potent endoprotease activity against fibrinogen at acid pH. May play an important role in extracellular matrix degradation. Involved in the release of thyroid hormone thyroxine (T4) by limited proteolysis of TG/thyroglobulin in the thyroid follicle lumen.
Gene References into Functions	the present study identified icariin as a novel therapeutic agent in DXM-induced cartilage injury in rats, and revealed that the activation of miR-206 targeting of cathepsin K may be responsible for the chondroprotective efficacy of icariin PMID: 29207029 cathepsin K, in both its secreted and lysosomal forms, may play a role in the degradation of Sertoli cell residual bodies PMID: 14585819 In conclusion, according to its ability to modulate hypotensive effects of kinins, we propose that cathepsin K is a kininase of biological relevance. PMID: 17181996 Comparison of the S2 site between rat and human cathepsin K sequences indicated that two S2 residues at Ser134 and Val160 in rat are varied to Ala and Leu, respectively, in the human enzyme. PMID: 18664521 The expression pattern of Ctsk in osteoclasts in physiological root resorption in the rat molar was determined. PMID: 18974601 Show More Hide All
Subcellular Location	Lysosome. Secreted. Apical cell membrane; Peripheral membrane protein; Extracellular side.
Protein Families	Peptidase C1 family
Database Links	KEGG: rno:29175 STRING: 10116.ENSRNOP00000028730 UniGene: Rn.5598

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