Cathepsin K is a protein in humans that is encoded by CTSK gene. Closely involved in osteoclastic bone resorption and may participate partially in the disorder of bone remodeling. Displays potent endoprotease activity against fibrinogen at acid pH. May play an important role in extracellular matrix degradation.
The following CTSK reagents supplied by CUSABIO are manufactured under a strict quality control system. Multiple applications have been validated and solid technical support is offered.
CTSK Antibodies for Rattus norvegicus (Rat)
Code | Product Name | Species Reactivity | Application |
---|---|---|---|
CSB-PA006192LA01RA | Ctsk Antibody |
Rat | ELISA, IHC |
CTSK Antibodies for Homo sapiens (Human)
Code | Product Name | Species Reactivity | Application |
---|---|---|---|
CSB-PA006192GA01HU | CTSK Antibody |
Human,Mouse,Rat | ELISA,WB,IHC |
CSB-PA15119A0Rb | CTSK Antibody |
Human | ELISA |
CSB-PA006192ESR1HU | CTSK Antibody |
Human, Rat | ELISA, WB, IHC |
CSB-PA039740 | CTSK Antibody |
Human,Mouse,Rat | ELISA,IHC |
CSB-PA145433 | CTSK Antibody |
Human,Mouse,Rat | ELISA,IHC |
CTSK Antibodies for Mus musculus (Mouse)
Code | Product Name | Species Reactivity | Application |
---|---|---|---|
CSB-PA006192ZA01MO | Ctsk Antibody |
Mus musculus | ELISA, WB (ensure identification of antigen) |
CTSK Antibodies for Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
Code | Product Name | Species Reactivity | Application |
---|---|---|---|
CSB-PA006192ZA01MOV | CTSK Antibody |
Macaca fascicularis | ELISA, WB (ensure identification of antigen) |
CTSK Antibodies for Canis lupus familiaris (Dog) (Canis familiaris)
Code | Product Name | Species Reactivity | Application |
---|---|---|---|
CSB-PA006192ZA01DO | CTSK Antibody |
Canis lupus familiaris | ELISA, WB (ensure identification of antigen) |
CTSK Antibodies for Oryctolagus cuniculus (Rabbit)
Code | Product Name | Species Reactivity | Application |
---|---|---|---|
CSB-PA006192ZA01RB | CTSK Antibody |
Oryctolagus cuniculus | ELISA, WB (ensure identification of antigen) |
CTSK Proteins for Homo sapiens (Human)
Code | Product Name | Source |
---|---|---|
CSB-YP006192HU | Recombinant Human Cathepsin K(CTSK) |
Yeast |
CSB-BP006192HU CSB-MP006192HU CSB-EP006192HU-B |
Recombinant Human Cathepsin K(CTSK) |
Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CSB-EP006192HU | Recombinant Human Cathepsin K(CTSK) |
E.coli |
CTSK Proteins for Oryctolagus cuniculus (Rabbit)
Code | Product Name | Source |
---|---|---|
CSB-YP006192RB CSB-BP006192RB CSB-MP006192RB CSB-EP006192RB-B |
Recombinant Rabbit Cathepsin K(CTSK) |
Yeast Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CSB-EP006192RB | Recombinant Rabbit Cathepsin K(Ctsk) |
E.coli |
CTSK Proteins for Mus musculus (Mouse)
Code | Product Name | Source |
---|---|---|
CSB-YP006192MO CSB-BP006192MO CSB-MP006192MO CSB-EP006192MO-B |
Recombinant Mouse Cathepsin K(Ctsk) |
Yeast Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CSB-EP006192MO | Recombinant Mouse Cathepsin K(Ctsk) |
E.coli |
CTSK Proteins for Macaca mulatta (Rhesus macaque)
Code | Product Name | Source |
---|---|---|
CSB-YP006192MOW CSB-EP006192MOW CSB-BP006192MOW CSB-MP006192MOW CSB-EP006192MOW-B |
Recombinant Macaca mulatta (Rhesus macaque) Cathepsin K(CTSK) |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CTSK Proteins for Rattus norvegicus (Rat)
Code | Product Name | Source |
---|---|---|
CSB-YP006192RA CSB-BP006192RA CSB-MP006192RA CSB-EP006192RA-B |
Recombinant Rat Cathepsin K(Ctsk) |
Yeast Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CSB-EP006192RA | Recombinant Rat Cathepsin K(Ctsk) |
E.coli |
CTSK Proteins for Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
Code | Product Name | Source |
---|---|---|
CSB-YP006192MOV CSB-BP006192MOV CSB-MP006192MOV CSB-EP006192MOV-B |
Recombinant Macaca fascicularis Cathepsin K(CTSK) |
Yeast Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CSB-EP006192MOV | Recombinant Macaca fascicularis Cathepsin K(CTSK) |
E.coli |
CTSK Proteins for Gallus gallus (Chicken)
Code | Product Name | Source |
---|---|---|
CSB-YP006192CH CSB-EP006192CH CSB-BP006192CH CSB-MP006192CH CSB-EP006192CH-B |
Recombinant Chicken Cathepsin K(CTSK) |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CTSK Proteins for Sus scrofa (Pig)
Code | Product Name | Source |
---|---|---|
CSB-YP006192PI CSB-EP006192PI CSB-BP006192PI CSB-MP006192PI CSB-EP006192PI-B |
Recombinant Pig Cathepsin K(CTSK) |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CTSK Proteins for Canis lupus familiaris (Dog) (Canis familiaris)
Code | Product Name | Source |
---|---|---|
CSB-YP006192DO CSB-BP006192DO CSB-MP006192DO CSB-EP006192DO-B |
Recombinant Dog Cathepsin K(CTSK) |
Yeast Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CSB-EP006192DO | Recombinant Dog Cathepsin K(CTSK) |
E.coli |
CTSK Proteins for Bos taurus (Bovine)
Code | Product Name | Source |
---|---|---|
CSB-YP006192BO CSB-EP006192BO CSB-BP006192BO CSB-MP006192BO CSB-EP006192BO-B |
Recombinant Bovine Cathepsin K(CTSK) |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CTSK ELISA Kit for Homo sapiens (Human)
Code | Product Name | Sample Types | Sensitivity |
---|---|---|---|
CSB-E09438h | Human Cathepsin K,cath-K ELISA Kit |
serum, plasma, tissue homogenates | 1.95 pg/mL |
CTSK ELISA Kit for Mus musculus (Mouse)
Code | Product Name | Sample Types | Sensitivity |
---|---|---|---|
CSB-E09440m | Mouse Cathepsin K,cath-K ELISA Kit |
serum, plasma, tissue homogenates | 3.9 pg/mL |
CTSK ELISA Kit for Rattus norvegicus (Rat)
Code | Product Name | Sample Types | Sensitivity |
---|---|---|---|
CSB-E09439r | Rat Cathepsin K,cath-K ELISA Kit |
serum, plasma, tissue homogenates | 3.9 pg/mL |
Cathepsin K (CTSK), a cysteine endopeptidase with a preference for non-aromatic hydrophobic residues such as Leu, Ile, Met, and Val, in its S2 binding pocket [1][2]. The enzyme is produced as a single-polypeptide- chain precursor pro-cathepsin K and is subsequently secreted as an inactive form. Mature pro-cathepsin K is activated upon the removal of the propeptide from the N-terminus through proteolysis under the acidic environment of the lysosomes [3]. Active CTSK is released into bone resorption lacunae. Consistently, CTSK is expressed predominantly in osteoclasts and plays a key role in bone remodeling and resorption [4][10]. Mutations in CTSK are associated with pycnodysostosis, a hereditary bone disorder in which osteoclast function in bone resorption is defective [5]. Hence, CTSK is regarded as a drug target for osteoporosis [11]. A multitude of novel inhibitors displaying high potency and specificity were developed and synthesized. The pH optimum of CTSK for synthetic substrates is between pH 5.5 and 6.5. CTSK can hydrolyze connective tissue proteins such as collagen and elastin. Chapman et al. identified that the elastolytic activity of CTSK exceeds that of pancreatic elastase at pH 5.5 [6]. Besides, CTSK can catalyze the hydrolysis of type I, II, and IV collagens [7]. Unlike other collagenolytic cathepsins, CTSK cleaves collagen in the telopeptides and at multiple sites within the native triple helix, generating fragments of various sizes [8]. The regulation of CTSK expression is controlled by RANKL (receptor activator of NF-κB ligand) [9]. CTSK is also reported to participate in lysosomal rupture-induced apoptosis.
[1] McGrath, M.E., et al. Crystal structure of human cathepsin K complexed with a potent inhibitor [J]. Nature Struct. Biol. 1997, 4: 106-109.
[2] Zhao, B.G., et al. Crystal structure of human osteoclast cathepsin K complex with E64 [J]. Nature Struct. Biol. 1997, 4: 109-111.
[3] Michael S. McQueney, Bernard Y. Amegadzie, et al. Autocatalytic Activation of Human Cathepsin K [J]. J Biol Chem 1997 272: 13955.
[4] Drake F.H., Dodds R.A., et al. Cathepsin K, but not cathepsins B, L, or S, is abundantly expressed in human osteoclasts [J]. J. Biol. Chem. 1996;271:12511-12516.
[5] Gelb, B.D., et al., 1996, Pycnodysostosis is a lysosomal disease caused by cathepsin K deficiency. Science 273: 1236-1238.
[6] Chapman HA, Riese RJ, et al. Emerging roles for cysteine proteases in human biology [J]. Annu Rev Physiol. 1997;59:63-88.
[7] Kafienah, W., et al. Human cathepsin K cleaves native type I and II collagens at the N-terminal end of the triple helix. Biochem. [J] J. 1998, 331: 727-732.
[8] P Garnero, O Borel, et al. The Collagenolytic Activity of Cathepsin K Is Unique Among Mammalian Proteinases [J]. J Biol Chem, 1998, 273 (48), 32347-52.
[9] Troen, B.R. The regulation of cathepsin K gen.e expression [J]. Ann. N. Y. Acad. Sci. 2006, 1068, 165-172.
[10] M. Asagiri, H. Takayanagi. The molecular understanding of osteoclast differentiation [J]. Bone, 40 (2007), pp. 251-264.
[11] Smith, W.W., and Abdelmeguid, S.S. Cathepsin K as a target for the treatment of osteoporosis [J]. Expert Opin. Ther. Patents 1999, 9: 683-694.