Rat Epithelial-Cadherin,E-Cad ELISA Kit

Code CSB-E07308r-IS
Size 96T,5×96T,10×96T
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Product Details

Target Name
cadherin 1, type 1, E-cadherin (epithelial)
Alternative Names
Cdh1Cadherin-1 ELISA kit; Epithelial cadherin ELISA kit; E-cadherin ELISA kit; Uvomorulin ELISA kit; CD antigen CD324) [Cleaved into: E-Cad/CTF1; E-Cad/CTF2; E-Cad/CTF3] ELISA kit
Abbreviation
Uniprot No.
Species
Rattus norvegicus (Rat)
Sample Types
serum, plasma, cell culture supernates, tissue homogenates, cell lysates
Detection Range
0.39ng/ml-25ng/ml
Sensitivity
0.195ng/ml
Assay Time
3.5h
Sample Loading Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cancer
Assay Principle
quantitative
Measurement
Sandwich
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

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Target Background

Function
(From Uniprot)
Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7.; E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production.
Gene References into Functions
  1. Cdk5-mediated inhibition of APC/C-Cdh1 switches on the cyclin D1-Cdk4-pRb pathway causing aberrant S-phase entry of postmitotic neurons PMID: 26658992
  2. astrocyte proliferation can be effectively suppressed by down-regulation of PFKFB3 through control of glycolytic flux, which is downstream of Cdh1 PMID: 26498254
  3. CIP4 overexpression represses E-cadherin expression by promoting beta-catenin translocation to the nucleus. PMID: 26287173
  4. Results suggest a regulatory function of E-cadherin that modulates Nrg1 signaling and promotes Schwann cell myelin formation PMID: 25988855
  5. E-cadherin expression and apoptosis may contribute to the development of Adriamycin nephrotoxicity PMID: 25801296
  6. Zinc can modulate N-cadherin-mediated adhesion. PMID: 25579424
  7. These results suggest that the anaphase-promoting complex-Cdh1 may play an important role in inhibiting axonal growth. PMID: 24634235
  8. results suggest that Cdh1 plays an important role in the regulation of reactive astrocyte proliferation induced by oxygen-glucose deprivation and reperfusion PMID: 23727062
  9. the total amount of E-cadherin expressed in animals subjected to diversion of the fecal stream for 6, 12, and 18 weeks was reduced in segments without a fecal stream. PMID: 23273176
  10. Cadmium disrupts the cadherin/beta-catenin complex in rat kidney cells, this effect leads to partial activation of beta-catenin-mediated gene transcription. PMID: 23080431
  11. E-cadherin is critical for collective sheet migration and is regulated by the chemokine CXCL12 protein during restitution PMID: 22549778
  12. The retaining of E-cadherin in cell-cell adhesion junctions affects the locomotor activity of transformed epithelial cells and plays an important role in their collective migration. PMID: 22288108
  13. E-cadherin can be expressed by a small population of rat undifferentiated spermatogonia in vivo and in vitro. PMID: 21853397
  14. beta-pix forms a complex with cadherin, beta-catenin, and scribble at synapses and enhances localized actin polymerization in rat hippocampal neurons. PMID: 22114281
  15. expression of E-cadherin and alpha-catenin was significantly associated with the ROS and degenerative changes in the seminiferous tubules. PMID: 21399649
  16. Expression of E-cadherin and claudin-4 has a circadian rhythm. The dynamic change in protein levels of E-cadherin and claudin-4 seems to coincide with that in the level of sodium excretion in rats. PMID: 20091493
  17. disappearance of the marginal actin bundle and rearrangements of AJs may change the adhesive function of E-cadherin and play an active role in migratory activity of carcinoma cells PMID: 19956566
  18. Fast dissociation kinetics between individual E-cadherin fragments revealed by flow chamber analysis PMID: 12032067
  19. Cadherin regulates dendritic spine morphogenesis and related synaptic functions, presumably cooperating with cadherin-independent adhesive mechanisms to maintain spine-axon contacts. PMID: 12123610
  20. E-cadherin expression is inversely proportional to tumor size in experimental liver metastases. PMID: 12127823
  21. calcitonin induced reduction in E-cadherin expression may remodel the adherens junctions between epithelial cells PMID: 12237291
  22. different regulation during axonal reorganization in the adult rat CNS PMID: 12387456
  23. In cochlea, E-cad expression is prominent in Hensen cells, Claudius cells, the outer sulcus, the future stria vascularis, and the epithelium (inner layer) of Reissner's membrane. PMID: 12640664
  24. Expression is decreased in iron-loaded hepatocytes PMID: 12651624
  25. Comparison of the expression patterns of E- and P-cadherins during wound healing of the epidermis strongly suggests that these two cadherins play distinct roles in re-epithelialization. PMID: 15771627
  26. It is concluded that CD2AP interacts with E-cadherin and co-localizes with F-actin in the leading edge of migrating cells, and significantly contributes to cell migration in restituting gastric epithelium. PMID: 15910750
  27. Ras signaling causes an upregulation of transcriptional repressors and subsequent downregulation of E-cadherin as a malignant phenotype is propagated PMID: 16153441
  28. Findings demonstrate a stimulatory role for E-cadherin in proliferative regulation, and identify a simple mechanism by which cell-cell contact may trigger or inhibit epithelial cell proliferation in different settings. PMID: 16682529
  29. results suggest that loss of beta-catenin precedes the onset of programmed cell death, loss of E-cadherin and extrusion from the villi. PMID: 16733663
  30. Heparin and enoxaparin can reduce E-cadherin expression in rat pregnancy which might modulate trophoblast invasion. PMID: 16997938
  31. Loss of E-cadherin expression is associated with bladder cancer progression PMID: 17167984
  32. A tight control of E-cadherin expression depending on the differentiation stage of the progenitor cells, is suggested. PMID: 17605082
  33. trefoil factor 3, in contrast to epidermal growth factor, enhanced a collective cell migration ensuring a precise coverage of the re-populated area avoiding gaps PMID: 17762162
  34. Cell adhesion via ECAD has distinct roles in the regulation of intercellular communication between beta-cells within islets, with potential repercussions for insulin secretion. PMID: 17982281
  35. These findings demonstrate a novel role for cell-cell adhesion in polarization, and have implications for wound healing and developmental patterning. PMID: 19258396
  36. These findings reveal a direct role for E-cadherin and for matrix metalloproteinases in causing epithelial-mesenchymal transition downstream of TGF-beta1 in fibrotic disease. PMID: 19590041

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Subcellular Location
Cell junction, adherens junction. Cell membrane; Single-pass type I membrane protein. Endosome. Golgi apparatus, trans-Golgi network.
Database Links
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