| Code | CSB-E07308r-IS |
| Size | 96T,5×96T,10×96T |
| Price | Request a Quote |
| Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase within six months until depleted. More details >> Interested in a trial size? Please leave a message below.
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Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<10% | ||||||
Three samples of known concentration were tested in twenty assays to assess. | ||||||
To assess the linearity of the assay, samples were spiked with high concentrations of rat E-Cad in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | ||||
1:1 | Average % | 95 | ||||
Range % | 88-100 | |||||
1:2 | Average % | 101 | ||||
Range % | 96-107 | |||||
1:4 | Average % | 87 | ||||
Range % | 83-94 | |||||
1:8 | Average % | 108 | ||||
Range % | 101-115 | |||||
The recovery of rat E-Cad spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 93 | 85-97 | ||||
EDTA plasma (n=4) | 102 | 95-106 | ||||
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
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ng/ml | OD1 | OD2 | Average | Corrected | ||
25 | 2.927 | 2.863 | 2.895 | 2.810 | ||
12.5 | 2.018 | 1.932 | 1.975 | 1.890 | ||
6.25 | 1.275 | 1.244 | 1.259 | 1.175 | ||
3.12 | 0.745 | 0.770 | 0.757 | 0.672 | ||
1.56 | 0.394 | 0.381 | 0.387 | 0.303 | ||
0.78 | 0.228 | 0.232 | 0.230 | 0.145 | ||
0.39 | 0.145 | 0.142 | 0.143 | 0.058 | ||
0 | 0.087 | 0.083 | 0.085 |
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