Rat Epithelial-Cadherin,E-Cad ELISA Kit

Instructions
Code CSB-E07308r
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name cadherin 1, type 1, E-cadherin (epithelial)
Alternative Names Cdh1Cadherin-1 ELISA kit; Epithelial cadherin ELISA kit; E-cadherin ELISA kit; Uvomorulin ELISA kit; CD antigen CD324) [Cleaved into: E-Cad/CTF1; E-Cad/CTF2; E-Cad/CTF3] ELISA kit
Abbreviation CDH1
Uniprot No. Q9R0T4
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 3.12 ng/mL-200 ng/mL
Sensitivity 0.78 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cancer
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat E-Cad in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 87
Range % 85-90
1:2 Average % 102
Range % 98-106
1:4 Average % 99
Range % 95-103
1:8 Average % 83
Range % 80-86
Recovery
The recovery of rat E-Cad spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 105 101-109
EDTA plasma (n=4) 97 93-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
200 2.750 2.798 2.774 2.591
100 2.045 1.968 2.007 1.824
50 1.401 1.358 1.380 1.197
25 0.920 0.967 0.944 0.761
12.5 0.576 0.598 0.587 0.404
6.25 0.445 0.468 0.457 0.274
3.12 0.307 0.312 0.310 0.127
0 0.182 0.183 0.183  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7.
Gene References into Functions
  1. Cdk5-mediated inhibition of APC/C-Cdh1 switches on the cyclin D1-Cdk4-pRb pathway causing aberrant S-phase entry of postmitotic neurons PMID: 26658992
  2. astrocyte proliferation can be effectively suppressed by down-regulation of PFKFB3 through control of glycolytic flux, which is downstream of Cdh1 PMID: 26498254
  3. CIP4 overexpression represses E-cadherin expression by promoting beta-catenin translocation to the nucleus. PMID: 26287173
  4. Results suggest a regulatory function of E-cadherin that modulates Nrg1 signaling and promotes Schwann cell myelin formation PMID: 25988855
  5. E-cadherin expression and apoptosis may contribute to the development of Adriamycin nephrotoxicity PMID: 25801296
  6. Zinc can modulate N-cadherin-mediated adhesion. PMID: 25579424
  7. These results suggest that the anaphase-promoting complex-Cdh1 may play an important role in inhibiting axonal growth. PMID: 24634235
  8. results suggest that Cdh1 plays an important role in the regulation of reactive astrocyte proliferation induced by oxygen-glucose deprivation and reperfusion PMID: 23727062
  9. the total amount of E-cadherin expressed in animals subjected to diversion of the fecal stream for 6, 12, and 18 weeks was reduced in segments without a fecal stream. PMID: 23273176
  10. Cadmium disrupts the cadherin/beta-catenin complex in rat kidney cells, this effect leads to partial activation of beta-catenin-mediated gene transcription. PMID: 23080431
  11. E-cadherin is critical for collective sheet migration and is regulated by the chemokine CXCL12 protein during restitution PMID: 22549778
  12. The retaining of E-cadherin in cell-cell adhesion junctions affects the locomotor activity of transformed epithelial cells and plays an important role in their collective migration. PMID: 22288108
  13. E-cadherin can be expressed by a small population of rat undifferentiated spermatogonia in vivo and in vitro. PMID: 21853397
  14. beta-pix forms a complex with cadherin, beta-catenin, and scribble at synapses and enhances localized actin polymerization in rat hippocampal neurons. PMID: 22114281
  15. expression of E-cadherin and alpha-catenin was significantly associated with the ROS and degenerative changes in the seminiferous tubules. PMID: 21399649
  16. Expression of E-cadherin and claudin-4 has a circadian rhythm. The dynamic change in protein levels of E-cadherin and claudin-4 seems to coincide with that in the level of sodium excretion in rats. PMID: 20091493
  17. disappearance of the marginal actin bundle and rearrangements of AJs may change the adhesive function of E-cadherin and play an active role in migratory activity of carcinoma cells PMID: 19956566
  18. Fast dissociation kinetics between individual E-cadherin fragments revealed by flow chamber analysis PMID: 12032067
  19. Cadherin regulates dendritic spine morphogenesis and related synaptic functions, presumably cooperating with cadherin-independent adhesive mechanisms to maintain spine-axon contacts. PMID: 12123610
  20. E-cadherin expression is inversely proportional to tumor size in experimental liver metastases. PMID: 12127823
  21. calcitonin induced reduction in E-cadherin expression may remodel the adherens junctions between epithelial cells PMID: 12237291
  22. different regulation during axonal reorganization in the adult rat CNS PMID: 12387456
  23. In cochlea, E-cad expression is prominent in Hensen cells, Claudius cells, the outer sulcus, the future stria vascularis, and the epithelium (inner layer) of Reissner's membrane. PMID: 12640664
  24. Expression is decreased in iron-loaded hepatocytes PMID: 12651624
  25. Comparison of the expression patterns of E- and P-cadherins during wound healing of the epidermis strongly suggests that these two cadherins play distinct roles in re-epithelialization. PMID: 15771627
  26. It is concluded that CD2AP interacts with E-cadherin and co-localizes with F-actin in the leading edge of migrating cells, and significantly contributes to cell migration in restituting gastric epithelium. PMID: 15910750
  27. Ras signaling causes an upregulation of transcriptional repressors and subsequent downregulation of E-cadherin as a malignant phenotype is propagated PMID: 16153441
  28. Findings demonstrate a stimulatory role for E-cadherin in proliferative regulation, and identify a simple mechanism by which cell-cell contact may trigger or inhibit epithelial cell proliferation in different settings. PMID: 16682529
  29. results suggest that loss of beta-catenin precedes the onset of programmed cell death, loss of E-cadherin and extrusion from the villi. PMID: 16733663
  30. Heparin and enoxaparin can reduce E-cadherin expression in rat pregnancy which might modulate trophoblast invasion. PMID: 16997938
  31. Loss of E-cadherin expression is associated with bladder cancer progression PMID: 17167984
  32. A tight control of E-cadherin expression depending on the differentiation stage of the progenitor cells, is suggested. PMID: 17605082
  33. trefoil factor 3, in contrast to epidermal growth factor, enhanced a collective cell migration ensuring a precise coverage of the re-populated area avoiding gaps PMID: 17762162
  34. Cell adhesion via ECAD has distinct roles in the regulation of intercellular communication between beta-cells within islets, with potential repercussions for insulin secretion. PMID: 17982281
  35. These findings demonstrate a novel role for cell-cell adhesion in polarization, and have implications for wound healing and developmental patterning. PMID: 19258396
  36. These findings reveal a direct role for E-cadherin and for matrix metalloproteinases in causing epithelial-mesenchymal transition downstream of TGF-beta1 in fibrotic disease. PMID: 19590041

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Subcellular Location Cell junction, Cell membrane, Single-pass type I membrane protein, Endosome, Golgi apparatus, trans-Golgi network
Database Links

KEGG: rno:83502

STRING: 10116.ENSRNOP00000027346

UniGene: Rn.1303

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