Rat Epithelial-Cadherin,E-Cad ELISA Kit

Code CSB-E07308r
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. Q9R0T4
Lead Time 3-5 working days
Abbreviation CDH1
Protein Biological Process 1 Cell Adhesion
Target Name cadherin 1, type 1, E-cadherin (epithelial)
Alias Arc-1, CD324, CDHE, ECAD, LCAM, UVO, E-Cadherin|cadherin 1, E-cadherin (epithelial)|cadherin 1, type 1|calcium-dependent adhesion protein, epithelial|cell-CAM 120/80|uvomorulin
Species Rattus norvegicus (Rat)
Protein Biological Process 3 Cell adhesion
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 3.12 ng/mL-200 ng/mL
Sensitivity 0.78 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cancer
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. Identified transcript variants arise from mutation at consensus splice sites.
HGNC 1748
RGD 69279
MGI 88354
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat E-Cad in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 87
Range % 85-90
1:2 Average % 102
Range % 98-106
1:4 Average % 99
Range % 95-103
1:8 Average % 83
Range % 80-86
Recovery
The recovery of rat E-Cad spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 105 101-109
EDTA plasma (n=4) 97 93-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
200 2.750 2.798 2.774 2.591
100 2.045 1.968 2.007 1.824
50 1.401 1.358 1.380 1.197
25 0.920 0.967 0.944 0.761
12.5 0.576 0.598 0.587 0.404
6.25 0.445 0.468 0.457 0.274
3.12 0.307 0.312 0.310 0.127
0 0.182 0.183 0.183  
References
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Function Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7.
Subcellular Location Cell junction, Cell membrane, Single-pass type I membrane protein, Endosome, Golgi apparatus, trans-Golgi network
Database Links

KEGG: rno:83502

STRING: 10116.ENSRNOP00000027346

UniGene: Rn.1303

Pathway Hippo signaling pathway
Adherens junction
Apelin signaling pathway
Rap1 signaling pathway

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